Xanthine oxidase, α-glucosidase and α-amylase inhibitory activities of the essential oil from Piper lolot: In vitro and in silico studies

Nguyen, Tan Khanh; Thuy Thi Tran, Linh; Ho Viet, Duc; Thai, Pham Hong; Ha, Tran Phuong; Ty, Pham Viet; Duc, Le Phu; Ton That Huu, Dat; Cuong, Le Canh Viet

doi:10.1016/j.heliyon.2023.e19148

Cited by 6 publications

(5 citation statements)

References 41 publications

(46 reference statements)

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“…3.6.3. α-Glucosidase Inhibition Assay α-Glucosidase inhibitory activity was determined as previously described [68] with minor modifications. In brief, 150 µL of 0.1 M phosphate buffer at pH 7.0 was added to each well and then 10 mg of the extracts dissolved in methanol to produce varied concentrations was added.…”

Section: α-Amylase Inhibition Assaymentioning

confidence: 99%

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

Alreshidi,

Abdulhakeem,

Badraoui

et al. 2023

Molecules

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Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of Pulicaria incisa (Lam.) DC. We also reported the molecular interaction between identified molecules and several receptors associated with antimicrobial and antibiofilm activities. A total of seventeen and thirteen compounds were identified in aqueous and methanolic extracts of P. incisa, respectively. The methanolic extract yielded a higher total content of polyphenols and flavonoids of about 84.80 ± 2.8 mg GAE/g and 28.30 ± 1.2 mg QE/g, respectively. Significant antibacterial activity was recorded for both extracts, with minimum inhibitory concentration (MIC) values ranging from 30 to 36 µg/mL, and the result was comparable to the reference antibiotic control. Antibiofilm assays revealed that both extracts were able to reduce the attachment of bacterial cells to 96-well plates, but the highest antibiofilm activity was recorded against Staphylococcus aureus. The methanolic extract also showed anti-enzymatic potency and high antioxidant activity, as demonstrated by all assays used, including DPPH, FRAP, and ABTS. These results were further validated by in silico approaches, particularly the molecular interaction of the identified compounds with the targeted receptors. These findings present P. incisa as a significant source of antibacterial, antibiofilm, antioxidant, and anti-enzymatic molecules.

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Section: α-Amylase Inhibition Assaymentioning

confidence: 99%

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

Alreshidi,

Abdulhakeem,

Badraoui

et al. 2023

Molecules

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“…14–17 However, to the best of our knowledge, no studies have been documented on the α -amylase and tyrosinase inhibitory effects of Paramignya species EOs, including P scandens EOs. In previous reports, the EOs from Piper nigrum (containing mainly β -caryophyllene, limonene, β -phellandrene, β -pinene, etc) 18 and Piper lolot (containing mainly β -caryophyllene, β -bisabolene, β -selinene, β -elemene, ( E )- β -ocimene, etc) 19 displayed good inhibition against α -amylase enzyme. In another study conducted by Yur et al, 20 it was claimed that Tanacetum haussknechtii EO contained α -pinene, β -pinene, santolinatriene, and borneol as the main compounds and showed significant anti-α-amylase and anti-tyrosinase activities.…”

Section: Resultsmentioning

confidence: 91%

Essential Oils From the Trunks and Leaves of Paramignya scandens (Griff.) Craib From Vietnam: Phytochemical Composition, In Vitro α-Amylase and Tyrosinase Inhibitory Activities and In Silico Molecular Docking Studies

Tran-Trung,

Thang,

Nguyen

et al. 2023

Natural Product Communications

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Objective/Background: This work aims to evaluate the chemical composition of Paramignya scandens essential oils (EOs), their in vitro α-amylase, tyrosinase inhibitory activities, and in silico molecular docking study. Methods: The EOs of P scandens trunks and leaves were extracted by hydrodistillation process and the chemical compositions were determined by gas chromatography-mass spectrometry. These EOs were also tested for in vitro α-amylase and tyrosinase inhibitory activities. Additionally, in silico molecular docking simulations on the main components of the EOs were performed. Results: The EO profiles of P scandens trunks and leaves included 21 (98.8%) and 34 (89.7%) components, respectively. The main compounds in the trunk EO were ( E)- β-caryophyllene (60.8%), α-humulene (13.0%), and germacrene D (7.4%). The predominant compounds of the leaf EO were caryophyllene oxide (16.7%), ( E) -β-caryophyllene (14.6%), spathulenol (12.2%), germacrene D (6.5%), and α-humulene (5.0%). Moreover, their α-amylase and tyrosinase inhibitory effects were demonstrated for the first time. In molecular docking results, ( E)- β-caryophyllene was the potential compound in EOs with the best binding affinity against the α-amylase enzyme, whereas spathulenol revealed the strongest binding ability with the tyrosinase enzyme. Furthermore, the assessment of potential interactions between the main components of the EOs and the selected proteins was elucidated. Conclusions: The results of this work revealed that EOs of P scandens trunks and leaves had α-amylase and tyrosinase inhibition properties and potential applications in nutraceuticals, medicine, and cosmetics.

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“…The antioxidant power of EOHT was evaluated using three tests, namely, the free radical scavenging DPPH assay, according to the protocol described in the study of Mssillou et al (2021). The ferric reducing antioxidant power (FRAP) assay, according to the protocol described in the study of El Abdali et al (2023); the measurement of total antioxidant capacity (TAC) according to the protocol reported in the study of Allali et al (2021).…”

Section: Evaluation Of Antioxidant Activitymentioning

confidence: 99%

Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.)

Agour,

Mssillou,

Allali

et al. 2023

Front. Chem.

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The present work aimed at characterizing the phytochemical composition of Haplophyllum tuberculatum essential oil (HTEO), assessing its antifungal activity against various fungal strains, evaluating its insecticidal and repulsive properties against Callosobruchus maculatus, and determine its antioxidant capacity. To this end, Gas chromatography-mass spectrometry analysis detected 34 compounds in HTEO, with β-Caryophyllene being the major constituent (36.94%). HTEO demonstrated predominantly modest antifungal effects, however, it sustains notable activity, particularly against Aspergillus flavus, with an inhibition rate of 76.50% ± 0.60%. Minimum inhibitory concentrations ranged from 20.53 ± 5.08 to 76.26 ± 5.08 mg/mL, effectively inhibiting fungal growth. Furthermore, the antifungal, and antioxidant activities of HTEO were evaluated in silico against the proteins Aspergillus flavus FAD glucose dehydrogenase, and beta-1,4-endoglucanase from Aspergillus niger, NAD(P)H Oxidase. Moreover, HTEO displayed strong insecticidal activity against C. maculatus, with contact and inhalation tests yielding LC50 values of 30.66 and 40.28 μL/100g, respectively, after 24 h of exposure. A dose of 5 μL/100g significantly reduced oviposition (48.85%) and inhibited emergence (45.15%) compared to the control group. Additionally, HTEO exhibited a high total antioxidant capacity of 758.34 mg AAE/g EO, highlighting its antioxidant potential. Insilico results showed that the antifungal activity of HTEO is mostly attributed to γ-Cadinene and p-Cymen-7-ol, while antioxidant is attributed to α-Terpinyl isobutyrate displayed. Overall, HTEO offers a sustainable and environmentally friendly alternative to synthetic products used to manage diseases.

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Xanthine oxidase, α-glucosidase and α-amylase inhibitory activities of the essential oil from Piper lolot: In vitro and in silico studies

Cited by 6 publications

References 41 publications

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis

Essential Oils From the Trunks and Leaves of Paramignya scandens (Griff.) Craib From Vietnam: Phytochemical Composition, In Vitro α-Amylase and Tyrosinase Inhibitory Activities and In Silico Molecular Docking Studies

Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.)

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