Xanthan gum stabilized gold nanoparticles: Characterization, biocompatibility, stability and cytotoxicity

Pooja, Deep; Panyaram, Sravani; Kulhari, Hitesh; Rachamalla, Shyam Sunder; Sistla, Ramakrishna

doi:10.1016/j.carbpol.2014.03.041

Cited by 189 publications

(86 citation statements)

References 33 publications

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“…DDN and CDN were assessed for hemolytic activity as reported earlier (Pooja et al 2014b). Briefly, mice RBCs were separated by centrifugation at 4000 rpm for 10 min at 4°C.…”

Section: Physical Stability Studiesmentioning

confidence: 99%

p-Hydroxy benzoic acid-conjugated dendrimer nanotherapeutics as potential carriers for targeted drug delivery to brain: an in vitro and in vivo evaluation

et al. 2015

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Dendrimers which are discrete nanostructures/nanoparticles are emerging as promising candidates for many nanomedicine applications. Ligandconjugated dendrimer facilitate the delivery of therapeutics in a targeted manner. Small molecules such as p-hydroxyl benzoic acid (pHBA) were found to have high affinity for sigma receptors which are prominent in most parts of central nervous system and tumors. The aim of this study was to synthesize pHBA-dendrimer conjugates as colloidal carrier for site-specific delivery of practically water insoluble drug, docetaxel (DTX) to brain tumors and to determine its targeting efficiency. pHBA, a small molecule ligand was coupled to the surface amine groups of generation 4-PAMAM dendrimer via a carbodiimide reaction and loaded with DTX. The conjugation was confirmed by 1 HNMR and FT-IR spectroscopy. In vitro release of drug from DTXloaded pHBA-conjugated dendrimer was found to be less as compared to unconjugated dendrimers. The prepared drug delivery system exhibited good physico-chemical stability and decrease in hemolytic toxicity. Cell viability and cell uptake studies were performed against U87MG human glioblastoma cells and formulations exerted considerable anticancer effect than plain drug. Conjugation of dendrimer with pHBA significantly enhanced the brain uptake of DTX which was shown by the recovery of a higher percentage of the dose from the brain following administration of pHBA-conjugated dendrimers compared with unconjugated dendrimer or formulation in clinical use (Taxotere Ò ). Therefore, pHBA conjugated dendrimers could be an efficient delivery vehicle for the targeting of anticancer drugs to brain tumors.

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“…DDN and CDN were assessed for hemolytic activity as reported earlier (Pooja et al 2014b). Briefly, mice RBCs were separated by centrifugation at 4000 rpm for 10 min at 4°C.…”

Section: Physical Stability Studiesmentioning

confidence: 99%

p-Hydroxy benzoic acid-conjugated dendrimer nanotherapeutics as potential carriers for targeted drug delivery to brain: an in vitro and in vivo evaluation

et al. 2015

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“…plantarum, and L. johnsonii although their productivities are much lower than species of Alcaligenes, Xanthomonas, Sphingomonas, and Leuconostoc, which are the microorganisms most used in industry for the production of curdlan, xanthan, gellan, and dextran (McIntosh et al 2005;Ö ner 2013). The xanthan gum is a component of dietary fiber and is also employed for the formulation of liquid or solid products in the pharmaceutical industry, in the latter case as an agent for controlled release (Tungland and Meyer 2002;Pooja et al 2014). Gellan gum is composed by repeated tetrasaccharide units of D-glucose, D-glucuronic acid, and L-rhamnose, is obtained from bacterium Pseudomonas elodea, and has been proved as a release vehicle of pharmaceuticals, cell carrier, material for guided bone regeneration, and wound dressing (Lee et al 2012).…”

Section: 2mentioning

confidence: 98%

Polysaccharide Production by Submerged Fermentation

Montoya

Vargas

2015

Polysaccharides

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“…For example, PEG-SH has been used to increase stability, minimize non-specific interactions of serum proteins with AuNPs, and improve platelet biocompatibility [76][77][78]. In addition, other biomolecules such as proteins, peptides, polysaccharides, and nucleic acids, have been used to stabilize AuNPs using similar strategies with different linkers [79][80][81]. For example, Helmut et al conjugated AuNPs to trypsin-NH 2 via immobilization through 1-ethyl-3-(3-dimethylaminopropylcarbodiimide/N-hydroxysuccinimide (EDC/NHS) bioconjugation onto functional carboxylic AuNPs (AuNP-S-Spacer-COO -) to generate AuNPs-trypsin with an amide linkage [82].…”

Section: Surface Chemistrymentioning

confidence: 98%

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

et al. 2015

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KEYWORDS siRNA delivery, RNA interference (RNAi)-based therapeutics, multifunctional nanoparticles, nanotoxicity of gold nanoparticles ABSTRACT RNA interference (RNAi) effectors such as small interfering RNA (siRNA) and micro RNA (miRNA) can selectively downregulate any gene implicated in the pathology of a disease. Therefore, RNAi-based therapies have immense potential for the treatment of a wide range of diseases. However, pharmacokinetic and pharmacodynamic studies have revealed that these therapeutic agents have poor bioactivity due to a number of factors, including insufficient plasma drug levels, short plasma half-lives, renal clearance, and hepatic metabolism. Non-viral delivery may facilitate the clinical application of siRNA-based therapeutics by helping to overcome these barriers. Recently, the potential of gold nanoparticles (AuNPs) as multifunctional carriers for transporting drugs, proteins, and genetic materials has been demonstrated. In this review, some of the key properties of AuNPs relevant to siRNA delivery, such as physical properties and surface chemistry have been described. In addition, the ability of AuNP-based formulation strategies to successfully overcome delivery barriers associated with siRNA, and the potential for this material to translate into safe and effective nanomedicines are critically discussed.

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Xanthan gum stabilized gold nanoparticles: Characterization, biocompatibility, stability and cytotoxicity

Cited by 189 publications

References 33 publications

p-Hydroxy benzoic acid-conjugated dendrimer nanotherapeutics as potential carriers for targeted drug delivery to brain: an in vitro and in vivo evaluation

p-Hydroxy benzoic acid-conjugated dendrimer nanotherapeutics as potential carriers for targeted drug delivery to brain: an in vitro and in vivo evaluation

Polysaccharide Production by Submerged Fermentation

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

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