Xác định một số loài động vật thân mềm vùng biển quần đảo Trường Sa, Việt Nam dựa trên trình tự nucleotide gen ty thể 16S rDNA

Abstract

DNA barcoding is one of the widely useful tools to identify the nomenclature of creatures. The target of this study is to identify 5 gastropods and 3 bivalves distributed in the Truong Sa Islands (Spratly Islands) of Vietnam using the 16S rDNA gene of mtDNA. The 16S gene fragment was isolated from the molluscs genome mtDNA by PCR technique. The PCR product was purified and sequenced. The 16S rDNA gene fragment was approximately 600 bp in length. The nucleotides of the 16S rDNA gene fragment were compared with those published on Genbank using the BLAST tool. The results showed that four gastropods named Cypraea tigris Linnaeus, 1758; Mauritia arabica Linnaeus, 1758; Oxymeris maculata Linnaeus, 1758; Drupa morum Röding, 1798; Haliotis ovina Gmelin, 1791 and three bivalves titled Tridacna squamosa Lamarck, 1819; Pinna atropurpurea G.B. Sowerby I, 1825; and Chama congregata Conrad, 1833 were identified. In which, T. squamosa and H. ovinawere classified as Vulnerable in Vietnam’s Red Data Book (2007). C. congregata is mainly distributed in South America, but in the study was first recorded in Vietnam’s sea. Using the 16S rDNA gene to determine molluscs in the Truong Sa Islands (Spratly Islands) of Vietnam contribited to accurately identifying creatures and provided basic data for further research in ecology, evolution and conservation of creatures.