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Suzina, N. E.; Mulyukin, A. L.; Лойко, Н. Г.; Kozlova, A. N.; Дмитриев, В. В.; Шорохова, А. П.; Горленко, В. М.; Дуда, В. И.; Эль-Регистан, Г. И.

doi:10.1023/a:1013183614830

Cited by 20 publications

(13 citation statements)

References 9 publications

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“…The high content of Na and K in the bacterial cells can be explained by the fact that bacteria were freeze-dried in a Na−K phosphate buffer at pH 7, which could increase concentrations of Na and K. As known, the membranes in lyophilized bacteria lose their barrier function . Therefore, during lyophilization Na and K ions must diffuse along their concentration gradients, and as a result, the lyophilization in media with a high concentration of either of these elements must lead to high content of this element in the cell.…”

Section: Resultsmentioning

confidence: 99%

Epithermal Neutron Activation Analysis of Cr(VI)-Reducer Basalt-Inhabiting Bacteria

et al. 2006

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Epithermal neutron activation analysis (ENAA) has been applied to study elemental composition of Cr(VI)-reducer bacteria isolated from polluted basalts from the Republic of Georgia. Cr(VI)-reducing ability of the bacteria was examined by electron spin resonance, demonstrating that the bacteria differ in their rates of Cr(VI) reduction. A well-pronounced correlation between the ability of the bacteria to accumulate Cr(V) and their ability to reduce Cr(V) to Cr(III) observed in our experiments is discussed. Elemental analysis of these bacteria also revealed that basalt-inhabiting bacteria are distinguished by relative contents of essential elements such as K, Na, Mg, Fe, Mn, Zn, and Co. A high rate of Cr(III) formation correlates with a high concentration of Co in the bacterium. ENAA detected some similarity in the elemental composition of the bacteria. The relatively high contents of Fe detected in the bacteria (140-340 microg/g of dry weight) indicate bacterial adaptation to the environmental conditions typical of the basalts. The concentrations of at least 12-19 different elements were determined in each type of bacteria simultaneously starting with the major to ultratrace elements. The range of concentrations spans over 8 orders of magnitude.

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Section: Resultsmentioning

confidence: 99%

Epithermal Neutron Activation Analysis of Cr(VI)-Reducer Basalt-Inhabiting Bacteria

et al. 2006

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“…One of the strategies to survive for prolonged periods is entering of microorganisms, especially non-spore-formers, to dormant state. Thus, the examinations of native bacterial populations in the Arctic and Antarctic permafrost samples of different genesis and age showed the presence of both gram-negative and grampositive bacteria in the form of dormant cystlike cells [32][33][34][35] . Cystlike cells in situ resembled dormant and stress-resistant cystlike cells of the type strains Micrococcus luteus and Arthrobacter globiformis, which maintained viability for prolonged periods and possessed experimentally undetectable respiration, and the elevated resistance to adverse conditions [36][37] .…”

Section: Other Important Methodological Aspectsmentioning

confidence: 99%

“…For dormant cells, fluorescent-antibody techniques may yield higher detection sensitivities 31 . As a good tool for direct detection of dormant microbial cells in situ, it was proposed ultrathin-sectioning and freeze-fracture electron microscopy with collected information on their specific morphological and ultrastructural features 34,35 . For now, it is enigmatic whether bacteria have existed or exist outside our planet, but in the future, when samples are available, the most convincing evidence of the microbial life may come from finding of living cells there.…”

Section: Other Important Methodological Aspectsmentioning

confidence: 99%

Microbiological methodology in astrobiology

Abyzov¹,

Герасименко²,

Hoover

et al. 2005

Astrobiology and Planetary Missions

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Searching for life in astromaterials to be delivered from the future missions to extraterrestrial bodies is undoubtedly related to studies of the properties and signatures of living microbial cells and microfossils on Earth. The Antarctic glacier and Earth permafrost habitats, where living microbial cells preserved viability for millennia years due to entering the anabiotic state, are often regarded as terrestrial analogs of Martian polar subsurface layers. For the future findings of viable microorganisms in samples from extraterrestrial objects, it is important to use a combined methodology that includes classical microbiological methods, plating onto nutrient media, direct epifluorescence and electron microscopy examinations, detection of the elemental composition of cells, PCR and FISH methods. Of great importance is to ensure authenticity of microorganisms (if any in studied samples) and to standardize the protocols used to minimize a risk of external contamination. Although the convincing evidence of extraterrestrial microbial life will may come from the discovery of living cells in astromaterials, biomorphs and microfossils must also be regarded as a target in search of life evidence bearing in mind a scenario that living microorganisms had not been preserved and underwent mineralization. Regarding the vital importance of distinguishing between biogenic and abiogenic signatures and between living and fossil microorganisms in analyzed samples, it is worthwhile to use previously developed approaches based on electron microscopy examinations and analysis of elemental composition of biomorphs in situ.

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“…Several studies have shown that [3,7] the addition of 4HR at low concentrations (up to 10 -4 M) to a population of stationary cell E. coli led to the formation of forms with increased stress resistance (the ability to maintain viability for a longer time than in control bacteria obtained under the same conditions, but not exposed to 4HR).…”

Section: Introductionmentioning

confidence: 99%

“…A dormant anabiotic (practically complete absence of metabolism) state, resembling the dormant state of cells under starvation stress, appears when 4HR is further added to the cell culture. [3,7].…”

Section: Introductionmentioning

confidence: 99%

The Structure of Dna in Anabiotic and Mummified Cells

Krupyanskii,

Kovalenko,

Loiko

et al. 2023

Preprint

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Investigations of structural organization of DNA for stressed (increased stress resistance), anabiotic and mummified cells obtained by introducing 4-hexylresorcinol at different concentrations for different stages of cell culture growth using diffraction of synchrotron radiation were done. Experimental studies allow us to conclude that 4-hexylresorcinol is the initiator of the cell transition to the anabiotic and mummified states at stationary state. At pre-stationary state of growth 4-hexylresorcinol initiates a transition of cells to a mummified state but not to anabiotic one. The structure of DNA within a cell in the anabiotic dormant state (practically complete absence of metabolism) and the dormant state (starvation stress) coincide (forms nanocrystalline structures). Our data suggest the universality of DNA condensation or the universality of DNA protection by a protein Dps for dormant state, regardless of the type of stress. Mummified state (the complete absence of metabolism) is very different in structure (has no ordering within a cell).

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Cited by 20 publications

References 9 publications

Epithermal Neutron Activation Analysis of Cr(VI)-Reducer Basalt-Inhabiting Bacteria

Epithermal Neutron Activation Analysis of Cr(VI)-Reducer Basalt-Inhabiting Bacteria

Microbiological methodology in astrobiology

The Structure of Dna in Anabiotic and Mummified Cells

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