Untitled

Kargul, George J.; Dudekula, Dawood B.; Qian, Yong; Lim, Mi Jung; Jaradat, Saied; Tanaka, Tetsuya; Carter, Mark G.; Ko, Minoru S.H.

doi:10.1038/88206

Cited by 27 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We selected an array consisting of 15 247 unique cDNAs derived from embryonic libraries (Kargul et al, 2001) comprising many known and novel genes involved in growth and development. The experimental protocol allowed us to reveal genes that were differentially expressed in tumor tissue vs normal brain as well as those differentially expressed in short latency (highgrade) vs long latency (low-grade) tumors.…”

Section: Discussionmentioning

confidence: 99%

Expression analysis of genes involved in brain tumor progression driven by retroviral insertional mutagenesis in mice

2005

View full text Add to dashboard Cite

Retroviral tagging previously identified putative cancercausing genes in a mouse brain tumor model where a recombinant Moloney murine leukemia virus encoding the platelet-derived growth factor B-chain (MMLV/PDGFB) was intracerebrally injected in newborn mice. In the present study, expression analysis using cDNA arrays revealed several similarities of virus-induced mouse gliomas with human brain tumors. Brain tumors with short latency contained on average 8.0 retroviral insertions and resembled human glioblastoma multiforme (GBM) whereas long-latency gliomas were of lower grade, similar to human oligodendroglioma (OD) and had 2.3 insertions per tumor. Several known and novel genes of tumor progression or cell markers were differentially expressed between OD-and GBM-like tumors. Array and quantitative real-time PCR analysis demonstrated elevated expression similar to Pdgfra of retrovirally tagged genes Abhd2, Ddr1, Fos, Ng2, Ppfibp1, Rad51b and Sulf2 in both glioma types compared to neonatal and adult normal brain. The retrovirally tagged genes Plekhb1, Prex1, Prkg2, Sox10 and 1200004M23Rik were upregulated in the tumors but had a different expression profile than Pdgfra whereas Rap1gap, Gli1, Neurl and Camk2b were downregulated in the tumors. The present study accentuates the proposed role of the retrovirally tagged genes in PDGF-driven gliomagenesis and indicates that insertional mutagenesis can promote glioma progression.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression analysis of genes involved in brain tumor progression driven by retroviral insertional mutagenesis in mice

2005

View full text Add to dashboard Cite

show abstract

“…In collaboration with the other members of the FANTOM consortium, including our colleagues from the MGD, we incorporated all cDNA data derived from the FANTOM project into GXD (38–40). We have also incorporated all other publicly available mouse cDNA and EST data, including cDNA data from the I.M.A.G.E consortium, the sets from the National Institute of Aging (NIA) (41,42) and the Mammalian Gene Collection (MGC) (43,44). We have loaded each clone's IDs and sequence information, including ESTs and any longer sequences that are known.…”

Section: Data Contentmentioning

confidence: 99%

The mouse Gene Expression Database (GXD): 2007 update

Smith

Finger

Hayamizu

et al. 2007

Nucleic Acids Research

View full text Add to dashboard Cite

The Gene Expression Database (GXD) provides the scientific community with an extensive and easily searchable database of gene expression information about the mouse. Its primary emphasis is on developmental studies. By integrating different types of expression data, GXD aims to provide comprehensive information about expression patterns of transcripts and proteins in wild-type and mutant mice. Integration with the other Mouse Genome Informatics (MGI) databases places the gene expression information in the context of genetic, sequence, functional and phenotypic information, enabling valuable insights into the molecular biology that underlies developmental and disease processes. In recent years the utility of GXD has been greatly enhanced by a large increase in data content, obtained from the literature and provided by researchers doing large-scale in situ and cDNA screens. In addition, we have continued to refine our query and display features to make it easier for users to interrogate the data. GXD is available through the MGI web site at or directly at .

show abstract

“…The NIA 15K clone set containing 15 247 sequences collected from embryonic cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryos at E7.5 and E12.5 female mesonephros/gonad) and newborn ovary tissues was utilized to generate the 15K NIA cDNA microarray (Ko et al, 1998;Ko et al, 2000;Tanaka et al, 2000;Kargul et al, 2001; sequence information is available at the web site of the National Institute on Aging (http:// lgsun.grc.nia.nih.gov/cDNA/15k.html). All clones are available as inserts of a modified pSPORT1 vector (Life Technologies, Rockville, MD, USA), propagated in clones of the Escherichia coli strain DH10B.…”

Section: Generation Of the 15k Nia Microarraymentioning

confidence: 99%