Osborne's work on the preparation of proteins was completed long before the development of the ph sical-chemical theory of acidity and the concept of solubility as a function of ionic strength. Nevertheless, his experimental handling of these factors, although empirical, was masterly.* Present-day methods show that casein yields about 22 per cent of glutamic acid. On the other hand, it is not impossible that Hlasiwetr and Habermann calculated the yield as glutamic acid hydrochloride, in which case they were not very much in error. Nevertheless, later research has shown that it is difficult to isolate more than half of the glutamic acid of casein by the method they used.