2016
DOI: 10.1073/pnas.1524607113
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X-ray structure of the MMTV-A nucleosome core

Abstract: The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar α-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-Å resolution X-ray structure of a nucleosome cor… Show more

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Cited by 36 publications
(42 citation statements)
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“…S5 and S6). Preference for a single orientation has been observed previously for an NCP with a non-palindromic DNA sequence containing a poly (dA·dT) tract as well as for the MMTV-A NCP (29,59). In the Telo-NCP refined map, the electron density showed well-resolved bases as well as distinct densities characterising purines and pyrimidines (Supplementary Figure S7).…”
Section: Crystal Structure Of the Telomeric Ncpsupporting
confidence: 68%
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“…S5 and S6). Preference for a single orientation has been observed previously for an NCP with a non-palindromic DNA sequence containing a poly (dA·dT) tract as well as for the MMTV-A NCP (29,59). In the Telo-NCP refined map, the electron density showed well-resolved bases as well as distinct densities characterising purines and pyrimidines (Supplementary Figure S7).…”
Section: Crystal Structure Of the Telomeric Ncpsupporting
confidence: 68%
“…By comparison, none of the Arg 17 aa of the H4 tails of the palindromic NCP-601L (62) are in contact with DNA (Figure 5c). We speculate that this strengthened tail interaction with DNA might contribute to the preference for a single orientation in the crystal lattice, as observed in the non-palindromic 147 bp MMTV-A NCP (29).…”
Section: The Basic H4 N-terminal Tail Interacts With the Ho Acidic Pamentioning
confidence: 72%
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“…To determine whether another type of nucleosome positioning sequence has a different sensitivity to the ncRNA, we performed a thermal stability assay using the nucleosome with 147 base pairs of the MMTV (mouse mammary tumor virus)-A DNA sequence 33 ( Supplementary Fig. 1d, e).…”
Section: Resultsmentioning
confidence: 99%
“…1A and Supplementary Table 1). All sequences are able to form nucleosomes of different stabilities and properties and have been extensively characterized (13)(14)(15)(16). In addition, effects into 5-methylcytosine (5mC) (17,18) and 5-hydroxymethylcytosine (5hmC) (19) on nucleosome occupancy have been investigated on the Widom 601 sequence, however it is unclear how 5fC affects nucleosome formation and stability.…”
mentioning
confidence: 99%