X-ray Structure of a Water-soluble Analog of the Membrane Protein Phospholamban: Sequence Determinants Defining the Topology of Tetrameric and Pentameric Coiled Coils

X-ray Crystal Structure of a TRPM Assembly Domain Reveals an Antiparallel Four-stranded Coiled-coil

“…These features classify TRPM7cc as an ‘a-d’ core coiled-coil. Relative to the three other known ‘a-d’ core four-stranded anti-parallel structures, WSPLP60, GCN4-E20C61, and Rop62, the amount of burial at the TRPM7cc positions that flank the core (‘e’ vs. ‘g’ and ‘b’ vs. ‘c’), is much more uniform (Table II). …”

Conformational Transition between Four and Five-stranded Phenylalanine Zippers Determined by a Local Packing Interaction

“…In many cases, this situation leads to the co-existence of mixed species in solution, and several reported examples of conformational transitions in coiled coils involve heterogeneous mixtures as one reaction component. 21,22,42,59,88 Structural selectivity is determined by an energetic balance between the requirements for stability and specificity. Currently, most simple design algorithms fail to discriminate explicitly among multiple states, to ensure specificity towards a target conformation.…”

“…The e and g residues are frequently charged, and their electrostatic interactions between supercoiled α-helices can influence the oligomerization state and strand polarity of canonical coiled coils. [13][14][15][16][17][18][19][20][21]88 Considerable progress has been made in understanding the structural determinants of parallel coiled-coil conformations. 7,12,22,23 The simplicity of the standard knobs-into-holes packing model has provided a foundation for several elegant computational methods that attempt to predict the core sidechain packing and backbone conformation of coiled Abbreviation used: SeMet, selenomethionine.…”

Designability landscape reveals sequence features that define axial helix rotation in four-helical homo-oligomeric antiparallel coiled-coil structures

“…For coiled coils, the used approaches range from manual and semi manual (Betz and DeGrado, 1996;Slovic et al, 2005), to fully-automated procedures Murphy et al, 2012). The design has been used to alter the properties of known coiled coils (Slovic et al, 2005), to build new structures recognizing other molecules and to form complex nanostructures not observed in the nature (Fletcher et al, 2013;Gradišar et al, 2013;Xu et al, 2013). In this study, we employed a design approach to provide a comprehensive characterization of axial helix rotation, one of the basic parameters of a coiled-coil structure, and to understand how this structural parameter is influenced by the sequence.…”