X-ray Scattering Study of Pike Olfactory Nerve: Elastic, Thermodynamic and Physiological Properties of the Axonal Membrane

Luzzati, Vittorio; Benoit, Évelyne; Charpentier, Gilles; Vachette, Patrice

doi:10.1016/j.jmb.2004.08.029

Cited by 13 publications

(7 citation statements)

References 20 publications

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“…1, Box B). Drugs may also be propelled within these channels by the structural changes that occur during depolarization and axonal propagation of the action potential in adjacent axons 84. Intracellular transport mechanisms involve the uptake of molecules into ORNs by passive diffusion, receptor‐mediated endocytosis or adsorptive endocytosis, followed by slower axonal transport, taking several hours to days for a drug to appear in the olfactory bulbs and other brain areas 85–87.…”

Section: Pathways and Mechanismsmentioning

confidence: 99%

Intranasal delivery to the central nervous system: Mechanisms and experimental considerations

Dhuria

Hanson

Frey

2010

Journal of Pharmaceutical Sciences

1,006

756

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Section: Pathways and Mechanismsmentioning

confidence: 99%

Intranasal delivery to the central nervous system: Mechanisms and experimental considerations

Dhuria

Hanson

Frey

2010

Journal of Pharmaceutical Sciences

1,006

756

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“…i av is the average of i(s j ) over the range 0.019 !s j !0.026 Å K1 . The values of i N and of the normalisation factor ½i av K i N were further refined in the course of the analysis (see the accompanying paper 6 ).…”

Section: Resultsmentioning

confidence: 99%

“…In the accompanying paper 6 we discuss the structural modulations induced in the axonal membrane by depolarisation, osmotic stress and a variety of drugs, and we approach the elastic, thermodynamic and physiological properties of the membrane.…”

Section: Introductionmentioning

confidence: 99%

X-ray Scattering Study of Pike Olfactory Nerve: Intensity of the Axonal Membrane, Solution of the Phase Problem and Electron Density Profile

Luzzati

Vachette

Benoit

et al. 2004

Journal of Molecular Biology

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“…. ) and to the highly sophisticated recording Plexiglas chamber, specifically made for simultaneous X-ray scattering and electrophysiological studies of pike olfactory CNAP [28]. Absence of stimulus artefacts was also observed when recording pike olfactory CNAP under similar conditions [27].…”

Section: Toxinsmentioning

confidence: 99%

Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin

et al. 2021

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Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetrodotoxin. The STX-sensitivity of compound nerve action potential (CNAP) recorded from oysters experimentally fed with Alexandrium minutum (toxic-alga-exposed oysters), or Tisochrysis lutea, a non-toxic microalga (control oysters), revealed that oysters could be separated into STX-resistant and STX-sensitive categories, regardless of the diet. Moreover, the percentage of toxin-sensitive nerves was lower, and the STX concentration necessary to inhibit 50% of CNAP higher, in recently toxic-alga-exposed oysters than in control bivalves. However, no obvious correlation was observed between nerve sensitivity to STX and the STX content in oyster digestive glands. None of the nerves isolated from wild and farmed oysters was detected to be sensitive to tetrodotoxin. In conclusion, this study highlights the good potential of cerebrovisceral nerves of Pacific oysters for electrophysiological and pharmacological studies. In addition, this study shows, for the first time, that C. gigas nerves have micromolar range of STX sensitivity. The STX sensitivity decreases, at least temporary, upon recent oyster exposure to dinoflagellates producing PST under natural, but not experimental environment.

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X-ray Scattering Study of Pike Olfactory Nerve: Elastic, Thermodynamic and Physiological Properties of the Axonal Membrane

Cited by 13 publications

References 20 publications

Intranasal delivery to the central nervous system: Mechanisms and experimental considerations

Intranasal delivery to the central nervous system: Mechanisms and experimental considerations

X-ray Scattering Study of Pike Olfactory Nerve: Intensity of the Axonal Membrane, Solution of the Phase Problem and Electron Density Profile

Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin

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