X-ray scattering of the Pt hexahalides of Cl, Br and I and Ta<sub>6</sub>Br<sub>12</sub>for XFEL

The reactivity of cis-diamminediiodidoplatinum(II), cis-PtI2(NH3)2, the iodo analogue of cisplatin, with hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry and X-ray crystallography. Interestingly, the study compound forms a stable 1:1 protein adduct for which the crystal structure was solved at 1.99 Å resolution. In this adduct, the Pt(II) center, upon release of one ammonia ligand, selectively coordinates to the imidazole of His15. Both iodide ligands remain bound to platinum, with this being a highly peculiar and unexpected feature. Notably, two equivalent modes of Pt(II) binding are possible that differ only in the location of I atoms with respect to ND1 of His15. The structure of the adduct was compared with that of HEWL-cisplatin, previously described; differences are stressed and their important mechanistic implications discussed.

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Cisplatin Binding to Proteins: Molecular Structure of the Ribonuclease A Adduct

Messori

Merlino

2014

Inorg. Chem.

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The crystal structure of the main adduct formed in the reaction between cisplatin and bovine pancreatic ribonuclease is reported here. Notably, in both of the protein molecules present in the asymmetric unit, platinum(II) binding takes place exclusively at the level of Met29. In one of the two molecules, the Gln28 side chain completes the platinum coordination sphere, anchoring the cisplatin fragment to the protein in a bidentate fashion. These results contain interesting implications for understanding the biological chemistry of this important drug.

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X-ray scattering of the Pt hexahalides of Cl, Br and I and Ta₆Br₁₂for XFEL

Cited by 4 publications

References 5 publications

Regulation of heteronuclear Pt–Ru complexes on the fibril formation and cytotoxicity of human islet amyloid polypeptide

Regulation of heteronuclear Pt–Ru complexes on the fibril formation and cytotoxicity of human islet amyloid polypeptide

Peculiar Features in the Crystal Structure of the Adduct Formed betweencis-PtI₂(NH₃)₂and Hen Egg White Lysozyme

Cisplatin Binding to Proteins: Molecular Structure of the Ribonuclease A Adduct

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X-ray scattering of the Pt hexahalides of Cl, Br and I and Ta6Br12for XFEL

Cited by 4 publications

References 5 publications

Regulation of heteronuclear Pt–Ru complexes on the fibril formation and cytotoxicity of human islet amyloid polypeptide

Regulation of heteronuclear Pt–Ru complexes on the fibril formation and cytotoxicity of human islet amyloid polypeptide

Peculiar Features in the Crystal Structure of the Adduct Formed betweencis-PtI2(NH3)2and Hen Egg White Lysozyme

Cisplatin Binding to Proteins: Molecular Structure of the Ribonuclease A Adduct

Contact Info

Product

Resources

About

X-ray scattering of the Pt hexahalides of Cl, Br and I and Ta₆Br₁₂for XFEL

Peculiar Features in the Crystal Structure of the Adduct Formed betweencis-PtI₂(NH₃)₂and Hen Egg White Lysozyme