X-ray phase-contrast tomography of cells manipulated with an optical stretcher
Jan-Philipp Burchert,
Jasper Frohn,
Ulrike Rölleke
et al.
Abstract:X-rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X-ray phase-contrast imaging and tomography is combined with an X-ray-compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X-ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron… Show more
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