X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis

Provinciali, Ginevra Begani; Pieroni, Nicola; Bukreeva, Inna; Fratini, Michela; Massimi, Lorenzo; Maugeri, Laura; Palermo, Francesca; Bardelli, Fabrizio; Mittone, Alberto; Bravin, Alberto; Gigli, Giuseppe; Gentile, Francesco; Fossaghi, Andrea; Riva, Nilo; Quattrini, Angelo; Cedola, Alessia

doi:10.1107/s1600577520006785

Cited by 10 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A key ability of XPCT is the possibility to generate a 3D multiscale image of the whole brain, which displays information on the NN and VN simultaneously ( Fratini et al, 2015 ; Bukreeva et al, 2017 ; Begani Provinciali et al, 2020 ). In previous works ( Cedola et al, 2017 ; Massimi et al, 2019 , 2020 ), we exploited this unique feature of XPCT to evaluate morphological alterations in the VN and NN, both in EAE and in the APP/PS1dE9 mouse model of Alzheimer’s disease (AD).…”

Section: Introductionmentioning

confidence: 99%

X-ray Phase Contrast Tomography Serves Preclinical Investigation of Neurodegenerative Diseases

et al. 2020

Self Cite

View full text Add to dashboard Cite

We report a qualitative study on central nervous system (CNS) damage that demonstrates the ability of X-ray phase contrast tomography (XPCT) to confirm data obtained with standard 2D methodology and permits the description of additional features that are not detected with 2D or other 3D techniques. In contrast to magnetic resonance or computed tomography, XPCT makes possible the high-resolution 3D imaging of soft tissues classically considered “invisible” to X-rays without the use of additional contrast agents, or without the need for intense processing of the tissue required by 2D techniques. Most importantly for studies of CNS diseases, XPCT enables a concomitant multi-scale 3D biomedical imaging of neuronal and vascular networks ranging from cells through to the CNS as a whole. In the last years, we have used XPCT to investigate neurodegenerative diseases, such as Alzheimer’s disease (AD) and multiple sclerosis (MS), to shed light on brain damage and extend the observations obtained with standard techniques. Here, we show the cutting-edge ability of XPCT to highlight in 3D, concomitantly, vascular occlusions and damages, close associations between plaques and damaged vessels, as well as dramatic changes induced at neuropathological level by treatment in AD mice. We corroborate data on the well-known blood-brain barrier dysfunction in the animal model of MS, experimental autoimmune encephalomyelitis, and further show its extent throughout the CNS axis and at the level of the single vessel/capillary.

show abstract

Section: Introductionmentioning

confidence: 99%

X-ray Phase Contrast Tomography Serves Preclinical Investigation of Neurodegenerative Diseases

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed, the dependency on fat oxidation is increased in ALS muscle cells, compared to healthy ones, suggesting a cell autonomous defect in energy metabolism [ 77 ]. In line with this observation, muscle-specific expression of mutated SOD1 G93A causes mitochondria abnormalities and dysfunction in vivo [ 82 , 83 ], parallel to neurovascular alterations, observed through X-ray-Phase-Contrast-Tomography (XPCT), at different stages of the disease [ 84 ]. XPCT demonstrated the ability to detect neuron alterations even in a pre-symptomatic state in SOD1 G93A mice.…”

Section: Metabolic Alteration In Neurodegenerative Diseasesmentioning

confidence: 93%

Metabolic Remodeling in Skeletal Muscle Atrophy as a Therapeutic Target

et al. 2021

Self Cite

View full text Add to dashboard Cite

Skeletal muscle is a highly responsive tissue, able to remodel its size and metabolism in response to external demand. Muscle fibers can vary from fast glycolytic to slow oxidative, and their frequency in a specific muscle is tightly regulated by fiber maturation, innervation, or external causes. Atrophic conditions, including aging, amyotrophic lateral sclerosis, and cancer-induced cachexia, differ in the causative factors and molecular signaling leading to muscle wasting; nevertheless, all of these conditions are characterized by metabolic remodeling, which contributes to the pathological progression of muscle atrophy. Here, we discuss how changes in muscle metabolism can be used as a therapeutic target and review the evidence in support of nutritional interventions and/or physical exercise as tools for counteracting muscle wasting in atrophic conditions.

show abstract

“…ALS is the most common and severe form of motor neuron diseases involving localized muscle weakness and respiratory symptoms. Superoxide dismutase 1 (SOD1) was the first identified ALS gene, upon which mouse models resembling ALS have been generated and extensively studied 5 . In the spinal cord transverse section (Fig.…”

Section: Xpct For the Investigation Of Neurogenerative Diseasesmentioning

confidence: 99%

Phase-Contrast Tomography with X-ray Hartmann wavefront sensor

Provinciali

Cedola

Rochefoucauld

et al. 2021

International Conference on X-Ray Lasers 2020

Self Cite

View full text Add to dashboard Cite

The Hartmann wavefront sensor is able to measure, separately and in absolute, the real δ and imaginary parts β of the X-ray refractive index. While combined with tomographic setup, Hartmann sensor opens many interesting opportunities behind the direct measurement of the material density. In order to handle the different ways of using an X-ray wavefront sensor in imaging, we developed 3D wave propagation model based on Fresnel propagator. The model is made in a way to manage any degree of spatial coherence of the source, thus enabling to model accurately experiments using tabletop source, high harmonic generation, plasma-based soft X-ray laser, synchrotron or X-ray free-electron laser. Beam divergence is described in a physical manner consistent with the spatial coherence.The capabilities of the Hartmann wavefront sensor will be compared with experimental results from in-line X-ray Phase Contrast Tomography.

show abstract

X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis

Cited by 10 publications

References 33 publications

X-ray Phase Contrast Tomography Serves Preclinical Investigation of Neurodegenerative Diseases

X-ray Phase Contrast Tomography Serves Preclinical Investigation of Neurodegenerative Diseases

Metabolic Remodeling in Skeletal Muscle Atrophy as a Therapeutic Target

Phase-Contrast Tomography with X-ray Hartmann wavefront sensor

Contact Info

Product

Resources

About