X-ray observations of single bio-supramolecular photochirogenesis

Chang, Jun; Nishijima, Masaki; Sekiguchi, Hiroshi; Ichiyanagi, Kouhei; Kawamura, Masahiro; Inoue, Yoshihisa; Sasaki, Y.

doi:10.1016/j.bpc.2018.07.003

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…However, knowledge of the orientation of AnpIBP movements is limited from the DXB experiments. DXT tracks the movement of the diffraction spots from the gold nanocrystals by using broadband X-ray and can be separately evaluated for the movements of the θ and χ directions in gold nanocrystals as tilting and twisting [ [1] , [2] , [3] , [4] , [5] , 30 ] ( Fig. 3 a).…”

Section: Resultsmentioning

confidence: 99%

Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking

Kawamura

Dong

Yue

et al. 2022

Biochemistry and Biophysics Reports

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The dynamic properties of protein molecules are involved in the relationship between their structure and function. Time-resolved X-ray observation enables capturing the structures of biomolecules with picometre-scale precision. However, this technique has yet to be implemented in living animals. Here, we examined diffracted X-ray blinking (DXB) and diffracted X-ray tracking (DXT) to observe the dynamics of a protein located on intestinal cells in adult Caenorhabditis elegans . This in vivo tissue-specific DXB was examined at temperatures from 20 °C to −10 °C for a recombinant ice-binding protein from Antarctomyces psychrotrophicus (AnpIBP) connected with the cells through a transmembrane CD4 protein equipped with a glycine-serine linker. AnpIBP inhibits ice growth at subzero temperatures by binding to ice crystals. We found that the rotational motion of AnpIBP decreases at −10 °C. In contrast, the motion of the AnpIBP mutant, which has a defective ice-binding ability, did not decrease at −10 °C. The twisting and tilting motional speeds of AnpIBPs measured above 5 °C by DXT were always higher than those of the defective AnpIBP mutant. These results suggest that wild-type AnpIBP is highly mobile in solution, and it is halted at subzero temperatures through ice binding. DXB and DXT allow for exploring protein behaviour in live animals with subnano resolution precision.

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Section: Resultsmentioning

confidence: 99%

Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking

Kawamura

Dong

Yue

et al. 2022

Biochemistry and Biophysics Reports

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Structural dynamics of a DNA-binding protein analyzed using diffracted X-ray tracking

Hosoe

Sekiguchi

Sasaki

et al. 2021

Biophysical Chemistry

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Tilting and rotational motions of silver halide crystal with diffracted X-ray blinking

Kawamura

Omata

Ishihara

et al. 2021

Sci Rep

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The dynamic properties of crystalline materials are important for understanding their local environment or individual single-grain motions. A new time-resolved observation method is required for use in many fields of investigation. Here, we developed in situ diffracted X-ray blinking to monitor high-resolution diffraction patterns from single-crystal grains with a 50 ms time resolution. The diffraction spots of single grains of silver halides and silver moved in the θ and χ directions during the photolysis chemical reaction. The movements of the spots represent tilting and rotational motions. The time trajectory of the diffraction intensity reflecting those motions was analysed by using single-pixel autocorrelation function (sp-ACF). Single-pixel ACF analysis revealed significant differences in the distributions of the ACF decay constants between silver halides, suggesting that the motions of single grains are different between them. The rotational diffusion coefficients for silver halides were estimated to be accurate at the level of approximately 0.1 to 0.3 pm2/s. Furthermore, newly formed silver grains on silver halides correlated with their ACF decay constants. Our high-resolution atomic scale measurement—sp-ACF analysis of diffraction patterns of individual grains—is useful for evaluating physical properties that are broadly applicable in physics, chemistry, and materials science.

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X-ray observations of single bio-supramolecular photochirogenesis

Cited by 3 publications

References 27 publications

Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking

Dynamic motions of ice-binding proteins in living Caenorhabditis elegans using diffracted X-ray blinking and tracking

Structural dynamics of a DNA-binding protein analyzed using diffracted X-ray tracking

Tilting and rotational motions of silver halide crystal with diffracted X-ray blinking

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