X-ray microtomography (microCT) of male genitalia of Nothybus kuznetsovorum (Nothybidae) and Cothornobata sp. (Micropezidae)

Galinskaya, Tatiana V.; Gafurova, Dina; Ovtshinnikova, O. G.

doi:10.3897/zookeys.744.22347

Cited by 9 publications

(3 citation statements)

References 18 publications

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“…In essence, a series of X-rays is taken from different angles in a complete circle around one plane of the specimen, either by rotating the X-ray source, as in a standard hospital CT suite, or by rotating the specimen in front of a static X-ray source, as for most μCT scientific applications. μCT has enormous potential for studies of insect science, addressing a range of questions from taxonomy [60,61], to anatomy [62], to in vivo muscle function [63], to exploring the host-parasite interface [64]. Two clear advantages of μCT over traditional histological techniques are that μCT is less time-consuming and, also, less invasive, allowing the virtual dissection of a sample in any plane (figure 2b,c).…”

Section: (C) Micro-computed Tomographymentioning

confidence: 99%

Visualization of insect metamorphosis

Hall

Martín‐Vega

2019

Phil. Trans. R. Soc. B

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Metamorphosis and, in particular, holometaboly, the development of organisms through a series of discrete stages (egg, larva, pupa, adult) that hardly resemble one another but are finely adapted to specific roles in the life cycle of the organism, has fascinated and mystified humans throughout history. However, it can be difficult to visualize the dramatic changes that occur during holometaboly without destructive sampling, traditionally through histology. However, advances in imaging technologies developed mainly for medical sciences have been applied to studies of insect metamorphosis over the past couple of decades. These include micro-computed tomography, magnetic resonance imaging and optical coherence tomography. A major advantage of these techniques is that they are rapid and non-destructive, enabling virtual dissection of an organism in any plane by anyone who has access to the image files and the necessary software. They can also be applied in some cases to visualize metamorphosis in vivo , including the periods of most rapid and dramatic morphological change. This review focusses on visualizing the intra-puparial holometabolous metamorphosis of cyclorraphous flies (Diptera), including the primary model organism for all genetic investigations, Drosophila melanogaster , and the blow flies of medical, veterinary and forensic importance, but also discusses similar studies on other insect orders. This article is part of the theme issue ‘The evolution of complete metamorphosis’.

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Section: (C) Micro-computed Tomographymentioning

confidence: 99%

Visualization of insect metamorphosis

Hall

Martín‐Vega

2019

Phil. Trans. R. Soc. B

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“…Among the morphological characters used in phylogenetic reconstructions and classification systems, the characters describing the morphology of the muscles of the genital and pregenital structures are usually more stable than those of the sclerites (Matsuda 1976;Ovtshinnikova 1989;Friedrich and Beutel 2008). Moreover, study of the muscles helps to clarify function and homology and reveals parallelisms in the pregenital and genital sclerites (Ovtshinnikova 1989(Ovtshinnikova , 1994Ovtshinnikova and Yeates 1998;Galinskaya and Ovtshinnikova 2015;Galinskaya et al 2018;Ovtshinnikova et al 2019).…”

Section: Introductionmentioning

confidence: 99%

The position of the Azeliinae in the Muscidae (Diptera) based on musculature of the male terminalia

Sorokina¹,

Ovtshinnikova²

2020

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The male genital and pregenital skeleton and musculature were studied in males of the following species of the Muscidae subfamily Azeliinae: Drymeia firthiana (Huckett, 1965), Drymeia longiseta Sorokina & Pont, 2015, Drymeia segnis (Holmgren, 1883), Thricops nigritellus (Zetterstedt, 1838), Thricops hirtulus (Zetterstedt, 1838), Hydrotaea dentipes (Fabricius, 1805), Muscina stabulans (Fallén, 1817), and Muscina levida (Harris, 1780). Descriptions and figures of the genital sclerites and muscles of D. firthiana and M. stabulans are given. A comparison was made between the genital segments and muscles of previously studied species of Mydaeinae and Muscinae and those of the Azeliinae. Based on the structure of the skeleton and muscles of syntergosternite VII + VIII and the phallapodeme muscles, significant differences were found between the subfamily Azeliinae and the subfamilies Mydaeinae and Muscinae. The basal position of the Azeliinae within the family Muscidae was confirmed. A comparison of the genital segments and muscles of the Muscidae with those of the Scathophagidae (Scathophaga stercoraria (Linnaeus, 1758)) and Anthomyiidae (Delia platura (Meigen, 1826)) was made. Tendencies in reduction of the pregenital segments and musculature, as well as of the phallapodeme muscles in the evolution of the Muscoidea have been revealed. The complete set of phallapodeme muscles in the Scathophagidae and Anthomyiidae corresponds to the basal state, and therefore the structure of the genital sclerites and muscles in the Muscidae shows a certain degree of reduction. The progressive changes in the Muscidae from the Azeliinae through the Mydaeinae to the Muscinae were traced.

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“…Study of the musculature is helpful not only for specifying the functions of genital sclerites, but also for revealing the homology of some poorly traced structures (Ovtshinnikova and Yeates 1998, Ovtshinnikova and Galinskaya 2016b, 2017, Galinskaya et al 2018). Based on morphogenetical regularities formulated by Matsuda (1976) and verified by Ovtshinnikova (1989) and Friedrich and Beutel (2008), characters associated with muscles are confirmed to be more stable than those associated with sclerites and therefore can be used successfully in phylogenetic studies; morphological series of different species are especially productive for such studies.…”

Section: Introductionmentioning

confidence: 99%

Skeleton and musculature of the male abdomen in Tanyderidae (Diptera, Nematocera) of the Southern Hemisphere

Ovtshinnikova¹,

Galinskaya²,

Lukashevich³

2018

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The structure of the male terminalia and their musculature of species of tanyderid genera Araucoderus Alexander, 1929 from Chile and Nothoderus Alexander, 1927 from Tasmania are examined and compared with each other and with published data on the likely relatives. The overall pattern of male terminalia of both genera is similar to those of most Southern Hemisphere genera, with simple curved gonostyli, lobe-like setose parameres, and setose cerci inconspicuous under the epandrium. Both genera have terminalia similarly rotated by 180° (and 90° as an intermediate stage); rotation may be either clockwise or counterclockwise. However, the similar patterns are realized differently: segment VIII is the decreased and asymmetrical due to completely membranose tergite VIII in Nothoderus (the first record of such modification in Tanyderidae), but narrow and symmetrical in Araucoderus. Accordingly, pregenital muscles are very different between the genera. Based on localization of muscle attachment sites, the hypandrial origin of the stripe between gonocoxites is shown in both genera, and entire membranization of tergite VIII and partial membranization of hypoproct is shown in Nothoderus. Tanyderidae are characterized by highly specialized sclerites and muscles of male terminalia and provide no evidence of relationship with previously studied members of Psychodidae, Blephariceridae and Ptychopteridae.

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X-ray microtomography (microCT) of male genitalia of Nothybus kuznetsovorum (Nothybidae) and Cothornobata sp. (Micropezidae)

Cited by 9 publications

References 18 publications

Visualization of insect metamorphosis

Visualization of insect metamorphosis

The position of the Azeliinae in the Muscidae (Diptera) based on musculature of the male terminalia

Skeleton and musculature of the male abdomen in Tanyderidae (Diptera, Nematocera) of the Southern Hemisphere

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