X-ray micro-computed-tomography in pediatric surgery: a new tool for studying embryos

Brosig, Susann; Peukert, Nicole; Metzger, Roman; Schneider, Hartmut; Haak, Rainer; Gosemann, Jan‐Hendrik; Lacher, Martin; Kluth, Dietrich

doi:10.1007/s00383-017-4213-1

Cited by 5 publications

(10 citation statements)

References 18 publications

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“…Yet, we were able to precisely monitor the prenatal and postnatal development of liver volume in the rat. Separation of single lobes could not be performed due to insufficient resolution, which is in contrast to the excellent results in lung development (Brosig, 2017; Wilkins & Pack, 2013). This might be due to the morphologic denseness and constancy of the hepatic tissue with missing landmarks that allow automated separation of structures.…”

Section: Discussionmentioning

confidence: 99%

“…This technique allows scanning of wet embryos, which might reduce shrinkage. Nonetheless, applying CPD “virtual‐separation” of organs appeared to be more accurate and contrast‐enhanced compared to wet embryos (Brosig, 2017; Metscher, 2009; Wong et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…All samples were kept dry until scanning. Detailed processing was previously described by our group (Brosig, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…There is also still controversy on a standardized fixation protocol of embryos for optimal results in micro-CT scanning. Our group uses Bouin's fixation combined with critical point drying (CPD) in order to create contrast enhancement of soft tissues (Brosig, 2017). This technique has been applied in the assessment of abnormal fetal heart development, allowing excellent imaging and exact volumetric measurements (Happel et al, 2010).…”

Section: Role Of Micro-ct In Embryological Research and Technical Asp...mentioning

confidence: 99%

“…Later, it was applied in small animal imaging and many different research fields, like imaging of osseous or vascular structures and abdominal organs. More recently, the implications of micro-CT imaging for embryological research were highlighted by our group and others (Brosig, 2017;Schambach et al, 2010).…”

mentioning

confidence: 90%

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High resolution three‐dimensional imaging and measurement of lung, heart, liver, and diaphragmatic development in the fetal rat based on micro‐computed tomography (micro‐CT)

et al. 2020

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Understanding of normal fetal organ development is crucial for the evaluation of the pathogenesis of congenital anomalies. Various techniques have been used to generate imaging of fetal rat organogenesis, such as histological dissection with 3‐dimensional reconstruction and scanning electron microscopy. However, these techniques did not imply quantitative measurements of developing organs (volumes, surface areas of organs). Furthermore, a partial or total destruction of the embryos prior to analysis was inevitable. Recently, micro‐computed tomography (micro‐CT) has been established as a novel tool to investigate embryonic development in non‐dissected embryos of rodents. In this study, we used the micro‐CT technique to generate 4D datasets of rat embryos aged between embryonic day 15–22 and newborns. Lungs, hearts, diaphragms, and livers were digitally segmented in order to measure organ volumes and analyze organ development as well as generate high‐resolution 3D images. These data provide objective values compiling a 4D atlas of pulmonary, cardiac, diaphragmatic, and hepatic development in the fetal rat.

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Section: Discussionmentioning

confidence: 99%