X-ray induced rearrangements between germ-line limited and soma chromosomes of Acricotopus lucidus (Diptera, Chironomidae)

Staiber, Wolfgang; Thudium, D.

doi:10.1007/bf00115135

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…These results strongly support our earlier hypothesis (Staiber and Thudium 1986), that during evolution Ks have developed from the Ss by rearrangements and by the formation and accumulation of repetitive elements ± germ line-specific repetitive sequences as demonstrated in this investigation.…”

Section: Discussionsupporting

confidence: 93%

Isolation and chromosomal localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae)

1997

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In the chironomid Acricotopus lucidus, parts of the genome, the germ line-limited chromosomes, are eliminated from the future soma cells during early cleavage divisions. A highly repetitive, germ line-specific DNA sequence family was isolated, cloned and sequenced. The monomers of the tandemly repeated sequences range in size from 175 to 184 bp. Analysis of sequence variation allowed the further classification of the germ line-restricted repetitive DNA into two related subfamilies, A and B. Fluorescence in situ hybridization to gonial metaphases demonstrated that the sequence family is highly specific for the paracentromeric heterochromatin of the germ line-limited chromosomes. Restriction analysis of genomic soma DNA of A. lucidus revealed another tandem repetitive DNA sequence family with monomers of about 175 bp in length. These DNA elements are found only in the centromeric regions of all soma chromosomes and one exceptional germ line-limited chromosome by in situ hybridization to polytene soma chromosomes and gonial metaphase chromosomes. The sequences described here may be involved in recognition, distinction and behavior of soma and germ line-limited chromosomes during the complex chromosome cycle in A. lucidus and may be useful for the genetic and cytological analysis of the processes of elimination of the germ line-limited chromosomes in the soma and germ line.

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Section: Discussionsupporting

confidence: 93%

Isolation and chromosomal localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae)

1997

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“…A permanent preparation of polytene salivary gland chromosomes of an X-ray induced reciprocal translocation between soma chromosome SIII and a germ line limited chromosome (K) of A. lucidus made in 1980 (Staiber and Thudium, 1986) was used in this study for obtaining K chromatin by microdissection. The K section is translocated to the long arm of SIII (III/6) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A permanent preparation of polytene salivary gland chromosomes of Acricotopus lucidus (Diptera, Chironomidae) from April 1980 carrying an X-ray induced rearrangment between a germ line-limited chromosome and a somatic one was used for microdissection (Staiber and Thudium, 1986). For chromosome preparation larval salivary glands had been fixed in ethanol:glacial acetic acid (3:1, v/v; 2 h), stained with acetic-carmine (12 h) and aceticlactic orcein (1.5 h) and squashed in a drop of the latter.…”

Section: Sample Preparation and Microdissectionmentioning

confidence: 99%

“…In the last few years, chromosome painting analyses with S-specific probes, and the isolation and localization of tandem repetitive DNA families clearly demonstrated that in the orthocladiid Acricotopus lucidus the Ks have developed from the Ss by duplication, rearrangments and accumulation of germ line-specific repetitive sequences (Staiber et al, 1997;Staiber and Schiffkowski, 2000;Staiber and Wahl, 2002). First indications that the Ks are composed of S-homologous sections and heterochromatic segments resulted from X-ray induced K-S-rearrangments analysed in the polytene larval salivary gland chromosomes (Staiber and Thudium, 1986;Staiber, 1991).…”

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confidence: 99%

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Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Staiber

2002

Cytogenet Genome Res

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In Acricotopus lucidus (Diptera, Chironomidae) the germ line-limited chromosomes (Ks) have developed from the soma chromosomes (Ss) by endoreduplication, rearrangements and accumulation of germ line-specific repetitive sequences. For molecular analysis of specific small K sections, microdissection of metaphase Ks generally yields very limited amounts of DNA. In this study, K-specific DNA was microdissected from defined polytenized K sections of X-ray induced K-S-rearrangements of permanent salivary gland chromosome preparations and was then amplified by DOP-PCR. A new germ line-specific tandem repetitive DNA family was isolated by this way from a heterochromatic K segment, characterized and localized on the Ks by FISH. The repetitive elements are related to sequences of earlier described K-specific tandem repetitive DNA families in A. lucidus, but are located mainly in terminal heterochromatin bands of the two largest Ks and only to a limited degree in the paracentromeric K heterochromatin. This demonstrates that a collection of permanent preparations of K-S-rearrangements with polytenized heterochromatic and S-homologous K sections of A. lucidus can be used as a source for obtaining K sequences of defined K parts to investigate the molecular evolution of the Ks.

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“…Some authors see the Ks as descendants of the S complement by way of endopolyploidy (Nicklas 1960;Painter 1966;Kunz et al 1970). First evidence for this hypothesis came from the analysis of X-ray-induced K-S rearrangements in polytene salivary gland chromosomes of A. lucidus, demonstrating that the Ks contain sections with S-homologous banding patterns (Staiber and Thudium 1986;Staiber 1991b).…”

Section: Discussionmentioning

confidence: 96%

Structural evolution of the germ line-limited chromosomes in Acricotopus

Staiber

Schiffkowski

2000

Chromosoma

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The elimination of chromatin or whole chromosomes from the future somatic nuclei during germ line-soma differentiation in early embryogenesis is a genetic phenomenon found in a wide variety of animal species. Less is known about the origin, structure, and function of the germ line-limited chromosomes. In the chironomid Acricotopus lucidus fluorescence in situ hybridization (FISH) with labeled soma DNA to "Keimbahn" chromosomes (Ks) and soma chromosomes (Ss) of spermatogonial mitoses revealed that each of the nine different K types possesses large S-homologous sections, mostly in the distal parts of both chromosome arms. Painting probes of the three Ss and of each of their chromosome arms were generated by microdissection of polytene salivary gland chromosomes and subsequent amplification by the degenerate oligonucleotide-primed polymerase chain reaction. Multicolor FISH demonstrated that each of the Ks, with the exception of one K type, was painted by only one of the three S probes. Furthermore, in seven Ks, one chromosome arm was painted by the long-arm probe and the other by the short-arm probe of the S concerned. The hybridization pattern strongly suggests that each of these K types is derived from a specific S. One function of the S-homologous K sections is thought to be determination of the regular occurrence of crossover events, with the resulting chiasmata in these sections ensuring correct segregation of the K homologs during meiosis. Reverse chromosome painting on polytene S sets with a probe generated from metaphase Ks corroborates the above results and produces conclusive evidence for the hypothesis that during evolution the Ks have developed from the Ss by endopolyploidization and rearrangements followed by the accumulation of germ line-specific repetitive DNA sequences in the centromeric regions.

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X-ray induced rearrangements between germ-line limited and soma chromosomes of Acricotopus lucidus (Diptera, Chironomidae)

Cited by 12 publications

References 19 publications

Isolation and chromosomal localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae)

Isolation and chromosomal localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae)

Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Structural evolution of the germ line-limited chromosomes in Acricotopus

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