X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood

Abstract

Using quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood.

Aloe veramodulates X-ray induced hematological and splenic tissue damage in mice

“…64 On the contrary, some reports indicate no change in RBCs and Hb levels due to gamma-ray exposure in mice. 65,66 In the present study, RBC count and Hb levels remained unaltered after X-ray exposure, which might be due to the remarkable regenerative capacity of blood cells to recover in short periods of time. 67 In the present study, elevation in WBC counts was observed in X-ray exposed mice when compared to control counterparts.…”

Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells

“…As a highly sensitive and reliable method of gene expression profiling, qPCR array combines qRT-PCR and gene chips which can screen differentially expressed genes based on mRNA. qPCR array is a useful tool for studies on signaling pathway [ 27 , 28 ]. In agreement with these findings, in the current study, the results demonstrated that 44 genes (including MyD88-dependent and nondependent TLR4, NLRS, MARK, and NF- κ B) were upregulated after LPS stimulation.…”

How Macrophages Become Transcriptionally Dysregulated: A Hidden Impact of Antitumor Therapy

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“…High radiation doses reduce IRF2 and 5 levels increasing numbers of M2-like macrophages in the tumor [ 151 ]. At least for the high-dose irradiation IRF1 is suppressed in monocytes, while upregulated in macrophages, suggesting the different responses from infiltrated cells and TAMs [ 72 , 73 ].…”