X-ray fluorescence with synchrotron radiation to elemental analysis of lead and calcium content of primary teeth

Guerra, Carolina de Souza; Gerlach, Raquel Fernanda; Pinto, N; Cardoso, Simone C.; Moreira, Silvana; Almeida, André Pereira de; Peixoto, Iza Teixeira Alves; Meloni, Carlos Henrique; Mota, Carla Lemos; Oliveira, Luis Fernando; Braz, Delson; Barroso, R.C.

doi:10.1016/j.apradiso.2009.08.005

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…Moreover, superficial etching techniques allow researchers to obtain enamel samples without the need for using complex procedures to separate dentin from the enamel. As a matter of fact, we have been using a 20-s superficial enamel acid etching technique to collect superficial enamel samples for lead analysis (Gomes et al, 2004;Costa de Almeida et al, 2007;de Almeida et al, 2008;Costa de Almeida et al, 2009), since lead and other micro-elements such as fluoride, zinc, and strontium accumulate in this outer layer of the enamel (de Souza Guerra et al, 2010).…”

Section: Introductionmentioning

confidence: 98%

New techniques for the recovery of small amounts of mature enamel proteins

Porto

Laure

Sousa

et al. 2011

Journal of Archaeological Science

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Section: Introductionmentioning

confidence: 98%

New techniques for the recovery of small amounts of mature enamel proteins

Porto

Laure

Sousa

et al. 2011

Journal of Archaeological Science

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“…Non-invasive in vitro techniques, such as microbeam synchrotron radiation X-ray fluorescence (μ-SRXRF), have also been used for this purpose. [9,18] Although LA-ICP-MS has a relatively low detection limit, its slightly destructive nature affects histopathological examinations of the samples after the elemental analysis. The heterogeneity of the sample volume ablated could introduce large uncertainty in quantification.…”

Section: Introductionmentioning

confidence: 99%

Microdistribution of lead in human teeth using microbeam synchrotron radiation X-ray fluorescence (μ-SRXRF)

et al. 2016

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Lead (Pb) exposure is known to be associated with adverse effects on human health, especially during the prenatal period and early childhood. The Pb content in teeth has been suggested as a useful biomarker for the evaluation of cumulative Pb exposure. This study was designed to employ the microbeam synchrotron radiation X-ray fluorescence technique to determine the microdistribution of Pb within the tooth to evaluate the reliability of the technique and the effectiveness of tooth Pb as a biomarker of Pb exposure. The results showed that in the incisor sample, Pb primarily deposited in secondary dentine region close to the pulp and secondarily at enamel exterior. In addition, Pb colocalised with Zn, indicating a positive correlation between Pb and Zn. By contrast, in the two molar samples, Pb accumulated principally in the pulp, and secondarily in the enamel. At the same time, Pb in these two molar samples colocalised with Ca instead of Zn as was observed in the incisor sample. Several batches of line scans further confirmed the conclusions. The feasibility of using microbeam synchrotron radiation X-ray fluorescence to determine the microdistribution of Pb in teeth and of using the tooth Pb, especially in dentine, as a biomarker was discussed.

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“…Synchrotron radiation X-ray fluorescence spectroscopy (SR-XRF) fulfills most of the criteria for such analysis. It is a non-destructive, multi-elemental analysis technique [11] that does not require any complex pretreatment of samples [12]. Its microprobe has better elemental sensitivity - in the range of parts per million - than most charged particle probes.…”

Section: Introductionmentioning

confidence: 99%

Synchrotron Radiation X-Ray Microfluorescence Reveals Polarized Distribution of Atomic Elements during Differentiation of Pluripotent Stem Cells

et al. 2011

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The mechanisms underlying pluripotency and differentiation in embryonic and reprogrammed stem cells are unclear. In this work, we characterized the pluripotent state towards neural differentiated state through analysis of trace elements distribution using the Synchrotron Radiation X-ray Fluorescence Spectroscopy. Naive and neural-stimulated embryoid bodies (EB) derived from embryonic and induced pluripotent stem (ES and iPS) cells were irradiated with a spatial resolution of 20 µm to make elemental maps and qualitative chemical analyses. Results show that these embryo-like aggregates exhibit self-organization at the atomic level. Metallic elements content rises and consistent elemental polarization pattern of P and S in both mouse and human pluripotent stem cells were observed, indicating that neural differentiation and elemental polarization are strongly correlated.

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X-ray fluorescence with synchrotron radiation to elemental analysis of lead and calcium content of primary teeth

Cited by 20 publications

References 24 publications

New techniques for the recovery of small amounts of mature enamel proteins

New techniques for the recovery of small amounts of mature enamel proteins

Microdistribution of lead in human teeth using microbeam synchrotron radiation X-ray fluorescence (μ-SRXRF)

Synchrotron Radiation X-Ray Microfluorescence Reveals Polarized Distribution of Atomic Elements during Differentiation of Pluripotent Stem Cells

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