“…Although, we observed distinct sub-regions such as nucleoid core and macrodomains, within the nucleoid region in E. coli, their exact composition and protein:nucleic acid ratio cannot be determined with SXT alone. Nevertheless, our observations correlate with previous observations of longitudinal density bundling and radial confinement of E. coli nucleoid 18,38,39 . Similar to histones in eukaryotes, HU that binds non-sequencespecific DNA, is essential for bacterial nucleoid condensation that relates to global gene expression 3,40 .…”