X-ray diffractometry and electron microscopy of DNA from Bacillus subtilis bound on clay minerals

Khanna, M.; Yoder, M.; Calamai, Luca; Stotzky, G.

doi:10.1007/s10112-998-0001-3

Cited by 67 publications

(35 citation statements)

References 18 publications

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“…Upon the addition of divalent metal ions super coiled plasmid DNA forms relatively stable complexes with carbon nanotubes due to chelation. The degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo (Khanna et al, 1998). Transfection efficiency of these nanoparticles was then assessed on liver cells in vitro, using a plasmid containing a fusion of an enhanced green fluorescent protein (pCDNA-GFP) reporter gene [ Figure-10].…”

Section: Gene/drug Deliverymentioning

confidence: 99%

Silver Nanoparticles – Universal Multifunctional Nanoparticles for Bio Sensing, Imaging for Diagnostics and Targeted Drug Delivery for Therapeutic Applications

Sironmani¹,

Daniel²

2011

Drug Discovery and Development - Present and Future

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Section: Gene/drug Deliverymentioning

confidence: 99%

Silver Nanoparticles – Universal Multifunctional Nanoparticles for Bio Sensing, Imaging for Diagnostics and Targeted Drug Delivery for Therapeutic Applications

Sironmani¹,

Daniel²

2011

Drug Discovery and Development - Present and Future

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“…Strands of DNA possess a strong negative charge due to the phosphate groups present on each nucleotide monomer. It has been proposed that exchangeable surface cations may form bridges between the phosphates on DNA and the negatively charged tetrahedral silica layer on the clay surface 13,14. Interactions of the phosphates with the narrow edges of platelets, which contain exposed Al 3+ and Mg 2+ ions at octahedral lattice breakpoints has also been suggested, though this mechanism is less likely because the edges are primarily terminated with hydroxyls rather than cations 1,2…”

Section: Introductionmentioning

confidence: 99%

Analysis of Oligonucleotide DNA Binding and Sedimentation Properties of Montmorillonite Clay Using Ultraviolet Light Spectroscopy

et al. 2008

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Smectite clays such as montmorillonite form complexes with a variety of biomolecules, including the nucleic acids DNA and RNA. Most previous studies of DNA adsorption onto clay have relied upon spectrophotometric analysis after separation of free nucleic acids from bound complexes by centrifugation. In the current work we demonstrate that such studies produce a consistent error due to (a) incomplete sedimentation of montmorillonite and (b) strong absorbance of the remaining clay at 260 nm. Clay sedimentation efficiency was strongly dependent upon cation concentration (Na + or Mg 2+ ) and on the level of dispersion of the original suspension. An improved clay:DNA adsorption assay was developed and utilized to assess the impact of metal counterions on binding of singlestranded DNA to montmorillonite. X-ray diffraction demonstrated, for the first time, formation of intercalated structures consistent with orientation of the DNA strands parallel to the clay surface. Observed gallery spacings were found to closely match values calculated utilizing atomistic modeling techniques.

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“…Thus, understanding how DNA is adsorbed by soil particles is important for developing efficient methods of extraction. Many researches have used 2:1 layer phyllosilicates such as montmorillonite as adsorptive particle to study DNA adsorption in soils (4,5,9,10,16). However, the results obtained with phyllosilicates alone are not enough to understand the behavior of DNA molecules in variablecharged soils such as andosols.…”

mentioning

confidence: 99%

The Influence of Soil Organic Matter on DNA Adsorptions on Andosols

Saeki

Sakai

2009

Microb. Environ.

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The influence of soil organic matter on DNA adsorption in andosols was investigated using various andosol samples including hydrogen peroxide (H2O2)-treated, heated (400°C), and slurry-added soils. Remarkably less DNA was adsorbed in the slurry-added soil than the original soil. The increase in soil organic matter with the addition of slurry had an obvious negative influence on the adsorption. The decrease in organic matter with H 2 O 2 treatment slightly raised DNA adsorption per unit weight. Adsorption maxima estimated from a simple Langmuir equation were higher in the samples removed of organic matter by the H 2 O 2 treatment and heating at 400°C than in the untreated soil, although surface area was greatly decreased by both treatments. There was no correlation between the total carbon (T-C) content and the estimated DNA adsorption maxima of any of the soil samples. These results suggest little contribution of soil organic matter to DNA adsorption in andosols.

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X-ray diffractometry and electron microscopy of DNA from Bacillus subtilis bound on clay minerals

Cited by 67 publications

References 18 publications

Silver Nanoparticles – Universal Multifunctional Nanoparticles for Bio Sensing, Imaging for Diagnostics and Targeted Drug Delivery for Therapeutic Applications

Silver Nanoparticles – Universal Multifunctional Nanoparticles for Bio Sensing, Imaging for Diagnostics and Targeted Drug Delivery for Therapeutic Applications

Analysis of Oligonucleotide DNA Binding and Sedimentation Properties of Montmorillonite Clay Using Ultraviolet Light Spectroscopy

The Influence of Soil Organic Matter on DNA Adsorptions on Andosols

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