X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation
Matthew P. Agdanowski,
Roger Castells-Graells,
Michael R. Sawaya
et al.
Abstract:Imaging scaffolds composed of designed protein cages fused to designed ankyrin repeat proteins (DARPins) have enabled the structure determination of small proteins by cryogenic electron microscopy (cryo-EM). One particularly well characterized scaffold type is a symmetric tetrahedral assembly composed of 24 subunits, 12 A and 12 B, which has three cargo-binding DARPins positioned on each vertex. Here, the X-ray crystal structure of a representative tetrahedral scaffold in the apo state is reported at 3.8 Å res… Show more
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