X-Linked Osteogenesis Imperfecta Possibly Caused by a Novel Variant in PLS3

Abstract

Osteogenesis imperfecta (OI) represents a complex spectrum of genetic bone diseases that occur primarily due to mutations and deletions of the COL1A1 and COL1A2 genes. Recent molecular studies of the network of signaling pathways have contributed to a better understanding of bone remodeling and the pathogenesis of OI caused by mutations in many other genes associated with normal bone mineralization. In this paper, a case of a rare X-linked variant of OI with a change in the gene encoding plastin 3—a protein important for the regulation of the actin cytoskeleton, is presented. A 16-year-old patient developed ten bone fractures caused by minor trauma or injury, including a compression fracture of the second lumbar vertebra during his lifetime. Next-generation sequencing analysis did not show pathologically relevant deviations in the COL1A1 and COL1A2 genes. Targeted gene analyses (Skeletal disorder panel) of the patient, his father, mother and sister were then performed, detecting variants of uncertain significance (VUS) for genes PLS3, FN1 and COL11A2. A variant in the PLS3 gene were identified in the patient, his mother and sister. Since the PLS3 gene is located on the X chromosome, the mother and sister showed no signs of the disease. Although the variant in the PLS3 gene (c.685G>A (p.Gly229Arg)) has not yet been described in the literature, nor is its pathogenicity known, clinical findings combined with genetic testing showed that this variant may explain the cause of X-linked OI in our patient. This rare case of the PLS3 variant of X-linked OI might point to a novel target for personalized therapy in patients with this severe disease.

Insights and implications of sexual dimorphism in osteoporosis

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Tibial plateau fracture and RNA sequencing with osteogenesis imperfecta: a case report

“…OI is a common systemic connective tissue disorder caused by abnormal synthesis and variants in exon encoding and synthesis of type I collagen, which is essential for maintaining normal ECM functions ( 15 , 16 ). In this case, transcript quantification revealed that compared with the normal control, there was no expression of CoL1A1 in this OI patient, which might be the pathogenic factor in the patient.…”

Genotypes and Clinical Phenotypes of Osteogenesis Imperfecta

“…In conclusion, X-linked OI caused by PLS3 mutations was clinically distinguished by repeated fractures. Treatments for high blood pressure may assist these patients' bone mineral density increase 69 . However, more research is required to determine whether PLS3 mutations and phenotypes are related.…”