X‐irradiation of the ovaries of guinea‐pigs and its effect on subsequent pregnancies

Abstract

A previous publication (Genther, '31) dealt with the irradiation of the ovaries of guinea-pigs and its effect on the oestrous cycle. It was found, regardless of the nature of the 1 THE AMERICAN JOURNAL O F ANATOMY, VOL. 55, N O . 1 JULY, 1934 2 IDA T. GENTHER X-RAYED OVARIES AND SUBSEQUENT PREGNANCIES 3soon after irradiation, when the cycles were still apparently normal, and later when the cycles were very abnormal.IV. Irradiation of the ovaries of very young animals, from 1 week of age to sexual maturity, to observe the effects on the cycles and snbsequent pregnancies.The experiments extended over a Long period of time, so that both the early and late effects of the irradiation could be observed. MATERIALS AND METHODSThe majority of animals used were laboratory raised. Uniformity of behavior was shown in that sexual maturity was reached between the weights of 275 and 315 gm. Cycles were normal and regular with few exceptions. Many controls were kept under the same conditions as the irradiated ones.The irradiation was carried out at the Children's Hospital under the direction of Dr. William Doughty, roentgenologist ; the treatments were given by Mrs. Mary Ware and Miss Ida V. Holt, x-ray technicians. The following setup was used : Milliamps, 5 Peak kilovolts, 165 Distance, 50 em. Port, 19 em. Filters, 1 mm. A1 + 2 mm. Cu.A half hour exposure was equivalent to 360 r units, calculated on the basis of 12 r units per minute, which were accurately measured. The minimum sterilization dose consisted of six half hour exposures, or 2160 r units, given within a week, the Lighter treatment of four exposures, or 1440 r units, given on successive days.A specially constructed box permitted four or five animals to be treated a t one time. Since the irradiation was dorsal, the animals were allowed to sit in a natural position, being held in place by a wooden halter that fitted snugly over the body behind the fore legs. No anaesthetic was used, since they iisually rested quietly. The entire body was covered with lead plates, except for a small strip over the region of the ovaries. In almost all ovaries studied thus far, both of a pair had

USAF Radiation Laboratory: Quarterly Progress Report (1963)

“…There was no increase in activity in the livers of irradiated animals. Thus, the livers of hepatectomized rats exposed to 200 …”

“…The procedure used to teat the hypothesis was as follows. At time sero one muse was given 200 3,. Anoxia produced by exposing mice to an environmental oxygen concentration of about h.l6% increased the LEO of whole-body x-ray exposure to about 10OO0 r. Production of maoxia within 10 seconds after x-ray exposure gave no protective effect.…”