X-irradiation induces cell death in fetal fibroblasts

Beck, Michaël; Moreels, Marjan; Jacquet, P.; Oostveldt, Patrick Van; Vos, Winnok H. De; Baatout, Sarah

doi:10.3892/ijmm.2012.964

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…A dose-independent response on genome level, however, is not inconceivable and might reflect highly efficient DNA repair mechanisms even in cells exposed to a high dose of sparsely ionizing photon radiation. It was found that 99% of all DSBs are correctly repaired in cells after a radiation dose of 2 Gy [22] and that fetal fibroblasts undergo more efficient DNA repair after high-dose photon irradiation [23]. Since human fibroblasts are quite resistant to IR-induced apoptosis and undergo extended G1 checkpoint arrest for days after 10 Gy photon irradiation [24], our 10 Gy X irradiation will predominantly have resulted in reproductive cell death.…”

Section: Dose-response Correlationmentioning

confidence: 99%

“…Furthermore, cells that underwent apoptotic cell death (less than 1.5%; G. Schrock, unpublished observations; [24]) were likely lost from the sequenced population. Additionally, high dose-triggered more efficient DNA repair in fibroblasts [23] could have removed most of the damage after the 10 Gy dose. This may be one way to explain why the number of high-impact, non-lethal mutations were similar for the cells exposed to low or high dose of X radiation (Table 1).…”

Section: Dose-response Correlationmentioning

confidence: 99%

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Genome-Wide DNA Alterations in X-Irradiated Human Gingiva Fibroblasts

Nath

Hagenau

Weiß

et al. 2020

IJMS

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While ionizing radiation (IR) is a powerful tool in medical diagnostics, nuclear medicine, and radiology, it also is a serious threat to the integrity of genetic material. Mutagenic effects of IR to the human genome have long been the subject of research, yet still comparatively little is known about the genome-wide effects of IR exposure on the DNA-sequence level. In this study, we employed high throughput sequencing technologies to investigate IR-induced DNA alterations in human gingiva fibroblasts (HGF) that were acutely exposed to 0.5, 2, and 10 Gy of 240 kV X-radiation followed by repair times of 16 h or 7 days before whole-genome sequencing (WGS). Our analysis of the obtained WGS datasets revealed patterns of IR-induced variant (SNV and InDel) accumulation across the genome, within chromosomes as well as around the borders of topologically associating domains (TADs). Chromosome 19 consistently accumulated the highest SNVs and InDels events. Translocations showed variable patterns but with recurrent chromosomes of origin (e.g., Chr7 and Chr16). IR-induced InDels showed a relative increase in number relative to SNVs and a characteristic signature with respect to the frequency of triplet deletions in areas without repetitive or microhomology features. Overall experimental conditions and datasets the majority of SNVs per genome had no or little predicted functional impact with a maximum of 62, showing damaging potential. A dose-dependent effect of IR was surprisingly not apparent. We also observed a significant reduction in transition/transversion (Ti/Tv) ratios for IR-dependent SNVs, which could point to a contribution of the mismatch repair (MMR) system that strongly favors the repair of transitions over transversions, to the IR-induced DNA-damage response in human cells. Taken together, our results show the presence of distinguishable characteristic patterns of IR-induced DNA-alterations on a genome-wide level and implicate DNA-repair mechanisms in the formation of these signatures.

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Section: Dose-response Correlationmentioning

confidence: 99%

Section: Dose-response Correlationmentioning

confidence: 99%

Genome-Wide DNA Alterations in X-Irradiated Human Gingiva Fibroblasts

Nath

Hagenau

Weiß

et al. 2020

IJMS

View full text Add to dashboard Cite

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Bioelectrical Impedance as a Diagnostic Factor in the Clinical Practice and Prognostic Factor for Survival in Cancer Patients: Prediction, Accuracy and Reliability

Tuorkey¹

2012

J Biosens Bioelectron

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Bioimpedance analysis could provide a clear figure about changes in cells and tissues based on frequencydependent changes, due to their electrical resistances for the applied electrical current. This review explains the physical principle of bioimpedance. And to monitor the progression of radiation induced tissue injury, particularly in radiotherapies. The interaction of radiation with the biological tissues and a prediction for their earlier and later alterations due to exposure are discussed. As well as, an overview for tissue identification by bioelectrical impedance analysis (BIA) is proposed. Bioimpedance analysis "applications and its limitations" in the health care, clinical practice and prognosis of overall survival in cancer patients are discussed.

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X-irradiation induces cell death in fetal fibroblasts

Cited by 2 publications

References 17 publications

Genome-Wide DNA Alterations in X-Irradiated Human Gingiva Fibroblasts

Genome-Wide DNA Alterations in X-Irradiated Human Gingiva Fibroblasts

Bioelectrical Impedance as a Diagnostic Factor in the Clinical Practice and Prognostic Factor for Survival in Cancer Patients: Prediction, Accuracy and Reliability

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