X-chromosome inactivation in female newborns conceived by assisted reproductive technologies

Wu, Ed X.; Stanar, Paloma; Ma, Sai

doi:10.1016/j.fertnstert.2014.03.010

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…We found no evidence for difference in methylation between newborns conceived through non-ICSI IVF and natural means (p = 0.634) [17]. In a study comparing of X-inactivation in newborn placentas from 60 ICSI-conceived, 72 non-ICSI-conceived and 52 naturally conceived females, no evidence was found for a difference in DNA methylation patterns between the three groups [18].…”

contrasting

confidence: 67%

Are the Effects of IVF on DNA Methylation Driven by Intracytoplasmic Sperm Injection and Male Infertility?

Loke

Craig

2016

Epigenomics

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contrasting

confidence: 67%

Are the Effects of IVF on DNA Methylation Driven by Intracytoplasmic Sperm Injection and Male Infertility?

Loke

Craig

2016

Epigenomics

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“…In one study, XCI patterns in cord blood samples of 30 IVF and 44 naturally conceived female infants were analyzed but XCI skewing was not found to be significantly different in the two groups [84]. In another larger study with 60 ICSI, 73 IVF and 52 naturally conceived female newborns, XCI skewing was not found to be associated with the mode of conception [85].…”

Section: Examination Of Epigenetic Change In Candidate Genesmentioning

confidence: 99%

Epigenetic changes and assisted reproductive technologies

et al. 2019

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Children conceived by Assisted Reproductive Technologies (ART) are at moderately increased risk for a number of undesirable outcomes, including low birth weight. Whether the additional risk is associated with specific procedures used in ART or biological factors that are intrinsic to infertility has been the subject of much debate, as has the mechanism by which ART or infertility might influence this risk. The potential effect of ART clinical and laboratory procedures on the gamete and embryo epigenomes heads the list of mechanistic candidates that might explain the association between ART and undesirable clinical outcomes. The reason for this focus is that the developmental time points at which ART clinical and laboratory procedures are implemented are precisely the time points at which large-scale reorganization of the epigenome takes place during normal development. In this manuscript, we review the many human studies comparing the epigenomes of ART children with children conceived in vivo, as well as assess the potential of individual ART clinical and laboratory procedures to alter the epigenome.

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“…Complete HpaII digestion of genomic DNA prior to AR gene assay was confirmed by amplification of the 5' region of the MIC2 gene (an X-linked gene that escapes X-inactivation and should be completely digested by methylation-sensitive HpaII enzyme) (Anderson and Brown, 2002;Goodfellow et al, 1988) according to a modified protocol of Wu et al (Wu et al, 2014). Both, HpaII-digested and undigested samples (4 µl) were amplified with 1X reaction buffer with 1.5 mM MgCl2, 200 µM of each dNTP, 0.2 µM of each primer, and 0.75 U of Taq DNA Polymerase (Roche) in a total volume of 15 µl.…”

Section: X-inactivation Analysismentioning

confidence: 99%

Female Fabry disease patients and X-chromosome inactivation

Juchniewicz

Kłoska

Tylki‐Szymańska

et al. 2018

Gene

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Fabry disease is an X-linked inherited lysosomal storage disorder caused by mutations in the gene encoding α-galactosidase A (GLA). Once it was thought to affect only hemizygous males. Over the last fifteen years, research has shown that most females carrying mutated allele also develop symptoms, demonstrating a wide range of disease severity, from a virtually asymptomatic to more classical profile, with cardiac, renal, and cerebrovascular manifestations. This variable expression in females is thought to be influenced by the process of X-chromosome inactivation (XCI). The aim of this study was to assess severity of the clinical phenotype, to analyze XCI patterns, and to estimate their effect on disease manifestation in twelve female Fabry disease patients from five unrelated Polish families. Our analyses revealed that patients presented with the broad range of disease expression - from mild to severe, and their clinical involvement did not correlate with XCI profiles. Female carriers of the mutation in the GLA gene with the random XCI may present with the wide range of disease signs and symptoms. Thus, XCI is not a main factor in the phenotype variability of Fabry disease manifestation in heterozygous females.

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X-chromosome inactivation in female newborns conceived by assisted reproductive technologies

Cited by 6 publications

References 21 publications

Are the Effects of IVF on DNA Methylation Driven by Intracytoplasmic Sperm Injection and Male Infertility?

Are the Effects of IVF on DNA Methylation Driven by Intracytoplasmic Sperm Injection and Male Infertility?

Epigenetic changes and assisted reproductive technologies

Female Fabry disease patients and X-chromosome inactivation

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