X-autosome ratio and the behavior pattern of individual X-chromosomes in placental mammals

Ohno, Susumu; Beçak, Willy; Beçak, Maria Luiza

doi:10.1007/bf00326912

Cited by 175 publications

(67 citation statements)

References 33 publications

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“…This observation agrees with OHNO et al (1964), who postulated on the basis of their studies on sex-chromosomes of a variety of placental mammals that X and Y chromosomes have evolved from a homologous pair of autosomes. However, in Diplopoda, since no cytological studies have been done on the female, mainly owing to technique difficulties in obtaining chromosome preparations, the identification of male sex-chromosomes (XY) cannot be regarded as absolutely final.…”

Section: Discussionsupporting

confidence: 90%

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Achar¹

1986

Caryologia

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Section: Discussionsupporting

confidence: 90%

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Achar¹

1986

Caryologia

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“…The estimated base pair number for pig X chromosome was 155 x lo6 bp, while it is 154 x lo6 bp in the human equivalent (24). We feel that this result is not coincidental and corroborates Ohno's hypothesis (18) that the X chromosome in the great majority of placental mammals has retained the original size and also the genetic constitution of a common ancestor.…”

Section: (*Isupporting

confidence: 74%

“…Hybridization was performed in the same mixture except that dextran sulfate was at 5% and NaCl a t 0.6 M. A sufficient amount of radiolabelled probe was added to the hybridization mixture to provide about 1 x 106cpm/cm2, which represents 10 ng or less of DNA per ml. Hybridization was performed at 42" C for 18 …”

Section: Molecular Hybridizationmentioning

confidence: 99%

Swine chromosomes: Flow sorting and spot blot hybridization

et al. 1986

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Flow cytometry analysis was applied to swine chromosomes prepared from phytohemagglutinin (PHA) stimulated peripheral blood lymphocytes. Flow karyotypes from both sexes and from t(3;7) translocation carrier females were obtained. A certain number of chromosome pairs could be assigned to various peaks. In fact, 13 peaks were observed for 18 autosomal pairs plus X and Y. Moreover, abnormalties owing to the t(3;7) translocation were readily observable.The number of base pairs for chromosomes associated with the various peaks was estimated by comparison with human flow karyotypes.The following four peaks were thus sorted: (1) the peak assumed to represent the translocated chromosome 7 plus the normals associated with it; (2) the corresponding peak from a normal swine; (3) the peak assumed to contain among others the normal chromosome 7; and finally (4) the peak corresponding to swine chromosome 1.Chromosomes of each peak were collected on Pall Biodyne membrane. Following appropriate denaturation and prehybridization, the four samples were hybridized with a human leucocyte antigen (HLA) class I32P‐labelled cDNA probe, representing most of the coding sequence of the HLA B7 gene. The results confirmed previous data from other techniques that assigned the swine MHC(SLA) to chromosome 7.Subsequently, sorted samples were hybridized with a porcine genomic Interferon alpha probe in order to confirm the mapping of this gene family on porcine chromosome 1.

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“…Raudsepp et al (2000) proposed arranged autosomes into two groups nineteen pairs of meta-and submetacentrics and eleven pairs of acrocentrics. The donkey X chromosome forms 4.67% of the haploid genome which is close to the estimated 5% for the horse (Ohno et al, 1964;Raudsepp et al, 2000). The Y chromosome, an acrocentric chromosome, is the smallest (1.32% of the haploid genome length) (Raudsepp and Chowdhary, 1999).…”

Section: Introductionsupporting

confidence: 57%

The fluorescence in situ study of highly repeated DNA sequences in domestic horse (Equus caballus) and domestic donkey (Equus asinus) - Advantages and limits of usefulness in phylogenetic analyses

Bugno‐Poniewierska¹,

Wnuk²,

Witarski³

et al. 2009

J. Anim. Feed Sci.

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The application of chromosome banding and the new molecular cytogenetics techniques, in particular fluorescent in situ hybridization (FISH) using chromosome painting probes found early use for understanding the systematic and phylogenetic relationships of species, as well as for providing insights to the possible mechanisms underpinning speciation. Here, we showed an application of primed in situ DNA synthesis (PRINS) and fluorescent in situ hybridization for comparative detection of the domestic horse (Equus caballus) and the domestic donkey (Equus asinus) telomere, centromere and nucleolar-organizer region sequences. The number, distribution and kind of highly repeated DNA sequences in across species with regard to their advantage and limit usefulness in phylogenetic analyses are discussed.

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X-autosome ratio and the behavior pattern of individual X-chromosomes in placental mammals

Cited by 175 publications

References 33 publications

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Analysis of Male Meiosis in Seven Species of Indian Pill-Millipedes (Diplopoda: Myriapoda)

Swine chromosomes: Flow sorting and spot blot hybridization

The fluorescence <i>in situ</i> study of highly repeated DNA sequences in domestic horse (<i>Equus caballus</i>) and domestic donkey (<i>Equus asinus</i>) - Advantages and limits of usefulness in phylogenetic analyses

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