X and Y chromosomes of Aedes aegypti (L.) distinguished by Giemsa C-banding

Newton, M. E.; Southern, D. I.; Wood, R. J.

doi:10.1007/bf00284986

Cited by 60 publications

(41 citation statements)

References 14 publications

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“…Bandas C Las Iáminas sin tinción, obtenidas a partir de la técnica de squash y sometidas a un tiempo de maduración de 10 días, fueron sumergidas en una solución de ácido clorhídrico 0,2 N durante 30 minutos; luego de previos lavados con agua destilada, se trataron con hidróxido de bario al 5% a 53 "C por un tiempo de 15 segundos y, antes de ser coloreadas con Giemsa al 2%, se colocaron durante una hora en solución salina doblemente citratada a una temperatura de 60 "C (18,29,30).…”

Section: Preparación De Cromosomas Mitóticosunclassified

“…Tal vez donde mayor aplicación ha tenido una de estas técnicas, bandas C, ha sido en algunas especies del género Aedes (18,29,30), en cuyos cromosomas, además de poder identificar con este método el centrómero y la región de la heterocromatina constitutiva asociada a él, también ha sido posible visualizar una banda intercalar del locus sexual en los cromosomas homólogos del par 1, lo cual ha permitido, igualmente y por las características de esta banda de heterocromatina facultativa, diferenciar las especies estudiadas e inferir sus relaciones filogenéticas (29).…”

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Comparación citogenética de Psorophora confinnis (Diptera: Culicidae) en cepas representativas de dos poblaciones colombianas

et al. 2000

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Con el propósito de comparar citogenéticamente dos cepas colombianas del mosquito Psorophora confinnis, procedentes de Lorica (Córdoba) y Granada (Meta), se efectuó un estudio de caracterización cariológica. A partir de tejidos cerebrales de lamas de IV estadio, se prepararon los cromosomas mitóticos mediante la técnica de aplastamiento (squash) del tejido. Se efectuaron las mediciones cromosómicas. atendiendo a los siguientes parámetros: brazo corto. brazo largo, relación de brazos. longitud total, longitud relativa, índice centromérico y longitud relativa de los cromosomas. Se compararon los cálculos morfométricos y se aplicaron patrones estandarizados para clasificar los cromosomas en los cariotipos analizados. Se registraron diferencias altamente significativas en la longitud total cromosómica y también en los valores de los brazos cortos y largos de los cromosomas, en las metafases obtenidas de las dos cepas. Teniendo en cuenta los patrones de bandas C y G, se estableció correctamente la ubicación del centrómero y las reqiones heterocromáticas asociadas a él e. iqualmente. el número de bandas en cada uno de los pares cromosómicos en el cariotipo de lascepas de Lorica y Granada. Los resultados de los datos morfométricos y de los fenotipos de bandas sugieren que Ps. confinnis existe en Colombia como un complejo de especies Palabras clave: Psorophora confinnis, cariotipos. cromosomas, bandas, morfometria cromosómica. Cytogenetic comparison of Psorophora confinnis (Diptera: Culicidae) in two Colombian representative population strainsWith the purpose of carrying out a cytogenetic comparison of the mosquito Psorophora confinnis from two Colombian strains (Lorica, Córdoba, and Granada, Meta) a karyological characterization study was carried out. Brain tissues from late 4'Vnstar larvae were used to obtain mitotic chromosomes through the squash technique. The chromosomal measurements were made to the following chromosomal characteristics: short arm, long arm, arm ratio, total length, relative length. centromeric index and relative length of the chromosomes. The morphometric data were compared. and standardized patterns were applied in order to classify the chromosomes in the analyzed karyotypes. They registered highly significant differences in the chromosomal total lengih ano a so ;n !he val-es of tnc snori aiia long arnis of itit chromosomcs n the tneiapriases of the two strains The C ano G uiinoinq Iiatlerns establisned thc corrccl cosil oti ~i f tnt? crniromcrr: and associated heterochromatic reg¡&;.as well as the number of barids in each chromosomal pair in the karyotypes from Lorica and Granada strains. The results of morphometric data and the banding phenotypes suggest that Ps. confinnis exists in Colombia as a species complex.

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Section: Preparación De Cromosomas Mitóticosunclassified

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Comparación citogenética de Psorophora confinnis (Diptera: Culicidae) en cepas representativas de dos poblaciones colombianas

et al. 2000

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“…The sex chromosomes can be differentiated cytologically by the Giemsa C-banding technique (Newton et al, 1974), the X (m) chromosome being marked with an m-linked centromeric band which is absent from the Y (M) chromosome. An intercalary band is also always present on the X chromosome but is sometimes absent from the Y. Markers on both arms of the sex chromosomes recombine with the M/m locus.…”

Section: Introductionmentioning

confidence: 99%

The genetic basis of resistance and sensitivity to the meiotic drive gene D in the mosquito Aedes aegypti L.

Wood

Ouda

1987

Genetica

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A study has been made on the genetic basis of meiotic drive at the Distorter (D) locus which, in coupling with the male-determining gene (or region) M on the Y chromosome, causes production of excess male progeny. Its effect is regulated by the sensitivity/resistance of the X chromosome. This study demonstrates that there are two major loci controlling resistance/sensitivity to MD: (1) the m gene (or region) on the X chromosome (allelic with M) which may be either mR or mS (resistant or sensitive), (2) the t (tolerance) gene (or genes) which recombines with m and, if present, largely counteracts the effect of mS. There is also evidence that MD itself is capable of limited adaptation. The conclusions were derived from using MD males of the T30 or ACCRA strains (from Trinidad and Ghana respectively). The work involved the use of the CHIPEI and RED strains with sensitive X chromosomes, the latter also carrying the t (tolerance) gene which is linked to re (red eye) and m (the sex-determining locus or region) but recombines with both. The implications of these findings for using MD as a method of population control are discussed.

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“…It is well known that the sex locus, M or m, is situated towards the centre of the genetic linkage map of chromosome 1 (Craig & Hickey, 1967;BhaUa & Craig, 1970), which is the shortest of the three chromosomes (Bhalla, 1973), and cytological studies have shown that it is also invariably inherited with the median centromere (Newton et al, 1974). Moreover, there is genetical evidence to suggest that the Distorter gene is similarly closely linked with the male determining gene or genes (Hickey & Craig, 1966a,b).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, an X chromosome is banded in the region of the centromere and in an intercalary position about half way along one arm, whereas a Y chromosome never shows a centromeric band, although it may possess an intercalary band. Advantage has also been taken of the fact that metaphase-I of meiosis in distorting (MD/mS+) males is disturbed and involves X chromosome breakage (Newton et al, 1974(Newton et al, , 1976, by means of which meiotic drive can be identified cytologically.…”

Section: Introductionmentioning

confidence: 99%

Cytological mapping of the M and D loci in the mosquito, Aedes aegypti (L.)

1978

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The separate identities of the male-determining factor, M, and the sex-linked Distorter gene, D, are established in an Accra strain ofAedes aegypti. Their positions in the Y chromosome are defined in relation to each other and to Giemsa C-bands. Thus, M is invariably inherited with the centromere, whereas D lies towards the intercalary band. Approximately 1.2% recombination occurs between M and D but, in a chromosome known for its distal localization of chiasmata, it is argued that the two are not necessarily as closely linked cytologically as this might imply. Evidence on the genetic effects of recombina. tion in the region of M and D is also considered.

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X and Y chromosomes of Aedes aegypti (L.) distinguished by Giemsa C-banding

Cited by 60 publications

References 14 publications

Comparación citogenética de Psorophora confinnis (Diptera: Culicidae) en cepas representativas de dos poblaciones colombianas

Comparación citogenética de Psorophora confinnis (Diptera: Culicidae) en cepas representativas de dos poblaciones colombianas

The genetic basis of resistance and sensitivity to the meiotic drive gene D in the mosquito Aedes aegypti L.

Cytological mapping of the M and D loci in the mosquito, Aedes aegypti (L.)

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