2000
DOI: 10.1177/002215540004800906
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X-34, A Fluorescent Derivative of Congo Red: A Novel Histochemical Stain for Alzheimer's Disease Pathology

Abstract: S U M M A R Y X-34, a lipophilic, highly fluorescent derivative of Congo red, was examined as a histochemical stain for pathological changes in Alzheimer's disease (AD). X-34 intensely stained neuritic and diffuse plaques, neurofibrillary tangles (NFTs), neuropil threads, and cerebrovascular amyloid. Comparison to standard methods of demonstrating AD pathology showed that X-34 correlated well with Bielschowsky and thioflavin-S staining. X-34 staining of NFTs correlated closely with anti-TAU antibody staining. … Show more

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Cited by 258 publications
(211 citation statements)
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“…Interestingly, the species of A␤ that coimmunoprecipitated with HSP-16 appeared to be predominantly multimeric. To determine whether this A␤͞HSP-16 interaction occurs in vivo, CL2006 transgenic animals were fixed and probed with mAb 4G8, the amyloid-specific dye X-34 (29), and anti-HSP-16 antibody. HSP-16 was found to tightly colocalize with immunoreactive A␤ deposits but not with the fully amyloidic A␤ detected by X-34.…”
Section: Resultsmentioning
confidence: 99%
“…Interestingly, the species of A␤ that coimmunoprecipitated with HSP-16 appeared to be predominantly multimeric. To determine whether this A␤͞HSP-16 interaction occurs in vivo, CL2006 transgenic animals were fixed and probed with mAb 4G8, the amyloid-specific dye X-34 (29), and anti-HSP-16 antibody. HSP-16 was found to tightly colocalize with immunoreactive A␤ deposits but not with the fully amyloidic A␤ detected by X-34.…”
Section: Resultsmentioning
confidence: 99%
“…6A, d and e) and fluorescent staining with thioflavin S and X-34 (Fig. 6A, a and c) (36). Positive neurons were abundant in brain and spinal cord of all tau-P301L mice of 9 months and older and absent or weak in CNS of younger tau-P301L mice.…”
Section: Tauopathy and Conformational Epitopes Characterize Tau-p301lmentioning
confidence: 82%
“…Finally, the sections were mounted in 10% Mowiol, 25% glycerol, 100 mM Tris⅐HCl (pH 8.5), 40 mg/ml triethylenediamine. Staining with X-34, a derivative of Congo Red that stains ␤-pleated proteins, was carried out essentially as described (36). Sections were washed twice for 2 min in PBS and incubated in 0.1 mM X-34 in 40% ethanol.…”
Section: Methodsmentioning
confidence: 99%
“…The relaxation times and relaxation rates of CR-BSA-(Gd-DTPA)n and Gd-DTPA in different solutions tissue (Steensma, 2001). Klunk and colleagues have been working for several years to develop amyloid-binding Congo red analogues as in vivo neuroimaging agents (Klunk et al, 1995;Styren et al, 2000). Makoto Higuchi reported a congo red derivative which had been used in 19 F and 1 H magnetic resonance imaging (MRI) for detection of amyloid b plaques (Higuchi et al, 2005).…”
Section: Discussionmentioning
confidence: 99%