WW domain binding protein 2 (WBP2) as an oncogene in breast cancer: mechanisms and therapeutic prospects—a narrative review

Liu, Yan; He, Enping; Zhang, Yanling; Liu, Yitong; Wang, Yingshuang; Chen, Siyu; Wu, Xinyu; Zeng, Youqing; Leng, Ping

doi:10.21037/gs-22-716

Cited by 3 publications

(6 citation statements)

References 99 publications

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“…CBP contains a conserved TAZ domain, while WBP2 features a conserved GRAM domain (Supporting Information: Figure ). The structural domains of CBP and WBP2 are also consistent with previous reports (Chen et al, 2017; De Guzman et al, 2004, 2005; Liu et al, 2022). Using the JASPAR core database, the TFBSs of NFYB on peak were predicted.…”

Section: Resultssupporting

confidence: 91%

“…Figure S6). The structural domains of CBP and WBP2 are also consistent with previous reports (Chen et al, 2017;De Guzman et al, 2004Liu et al, 2022). Using the JASPAR core database, the TFBSs of NFYB on peak were predicted.…”

Section: Sequence Analysis Of the Positive Clonessupporting

confidence: 86%

“…WBP2 is a transcriptional coactivator that contains a proline‐rich conserved sequence motif, PPxY, capable of binding to the WW domain (Liu et al, 2022). Research regarding the role of WBP2 in lipid metabolism is relatively unclear.…”

Section: Discussionmentioning

confidence: 99%

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Variation of gene expression of fatty acid acyl CoA reductase associated with wax secretion of a scale insect, Ericerus pela, and identification of its regulation factors through the accessible chromatin analyses and yeast one‐hybrid

Fu,

Shi,

et al. 2024

Arch Insect Biochem Physiol

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The Chinese white wax scale insect (CWWSI), Ericerus pela, can secret an amount of wax equivalent to their body weight. Previous studies demonstrated the fatty acyl‐CoA reductase (far3) plays a pivotal role in wax secretion of CWWSI. The high expression of far3 is crucial for the massive wax secretion. However, the transcription regulation of far3 was not clear. To identify regulatory factors that control the expression of far3, the assay for transposase‐accessible chromatin (ATAC) and yeast one‐hybrid (Y1H) were carried out in this study. The ATAC sequencing of the CWWSI at the early wax‐secretion stage ATAC‐seq resulted in 22.75 GB raw data, generated 75,827,225 clean reads and revealed 142,771 peaks. There was one significant peak in the 3 kb upstream regulation regions. The peak sequence is located between −1000 and −670 bp upstream of the far3 transcription start site, spanning a length of 331 bp. This peak sequence served as bait for creating the pAbAi‐peak recombinant vector, used in Y1H screenings to identify proteins interacting with far3 gene. The results indicate a successful CWWSI cDNA library construction with a capacity of 1.2 × 107 colony forming unit, a 95.8% recombination rate, and insert sizes between 1,000 and 2,000 bp. Self‐activation tests established that 100 ng/mL of AbA effectively inhibited bait vector self‐activation. Finally, a total of 88 positive clones were selected. After sequencing and removal of duplication, 63 unique clones were obtained from these screened colonies. By aligning the clone sequences with full‐length transcriptome and genome of CWWSI, the full‐length coding sequences of these clones were obtained. BlastX analysis identified a transcription factor, nuclear transcription factor Y beta, and two co‐activators, cAMP‐response‐element‐binding‐protein‐binding protein and WW domain binding protein 2. Reverse transcription quantitative polymerase chain reaction analysis confirmed that their expression patterns were consistent with the developmental stages preceding wax secretion and matched the wax secretion characteristics during ovulation periods. These results are beneficial for further research into the regulatory mechanisms of wax secretion of CWWSI.

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Section: Resultssupporting

confidence: 91%

Section: Sequence Analysis Of the Positive Clonessupporting

confidence: 86%

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Variation of gene expression of fatty acid acyl CoA reductase associated with wax secretion of a scale insect, Ericerus pela, and identification of its regulation factors through the accessible chromatin analyses and yeast one‐hybrid

Fu,

Shi,

et al. 2024

Arch Insect Biochem Physiol

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“…Overexpression of WBP2 has been observed in several types of cancer, including hepatocellular carcinoma (HCC), and is thought to contribute to tumour growth and progression [30]. WBP2 is involved in multiple tumour-promoting signalling pathways and plays a dominant role in tumorigenesis.…”

Section: Wbp2 Overexpression In Peripheral Blood Mononuclear Cells Of...mentioning

confidence: 99%

“…The overexpression of WBP2 in HCC is believed to contribute to tumour growth and progression by promoting cell proliferation, survival, and migration. WBP2 has been shown to interact with the oncogenic protein c-myc and enhance its transcriptional activity, promoting cell proliferation and survival [41]. WBP2 has also been shown to promote the migration and invasion of HCC cells by interacting with the actin cytoskeleton and regulating cell morphology.…”

Section: Wbp2 Overexpression In Peripheral Blood Mononuclear Cells Of...mentioning

confidence: 99%

Identification of Novel Biomarkers in Peripheral Blood Mononuclear Cells of Hepatocellular Carcinoma Patients

Sindhoora¹

2023

Preprint

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In this study, novel biomarkers in Blood Mononuclear Cells (PBMCs) of Hepatocellular Carcinoma (HCC) patients were identified through microarray data analysis. The problem that prompted the study was the lack of reliable biomarkers for early diagnosis and monitoring of HCC. The purpose of this study was to discover potential biomarkers in PBMCs of HCC patients that can be used for early diagnosis and monitoring of the disease. The main hypothesis was that there are genes that are overexpressed in PBMC of HCC patients compared to healthy individuals. The results showed that genes HBB, WBP2, HBA2, and HBA1 were overexpressed in PBMCs of HCC patients. Additionally, nine genes were found to be upregulated in HCC patients and had a relation between KEGG pathways of RA, suggesting a link between the two diseases. These genes are TLR4, IL1B, CXCL5, IL11, HLA-DQA1, HLA-DRA, LBT, ATP6V1B2 and ATP6V1C1. The Gene ontology analysis revealed biological processes used in the process of how these genes play a role in development of HCC. In conclusion, this study identified potential biomarkers in PBMC of HCC patients that can aid in early diagnosis and monitoring of the disease. The findings of this study have important implications for improving the clinical management of HCC patients.

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Function and prognosis analysis of nucleolus protein DCAF13 in breast cancer

Wang,

Fu,

Tian

et al. 2023

Transl Cancer Res

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Background Breast cancer is one of the main causes of death among women. RNA binding proteins (RBPs) play a crucial role in the progression of breast cancer, with increasingly detailed understanding of RBP functional molecular mechanisms in breast cancer, the functional research of RBPs may help elucidate the potential mechanisms of tumor occurrence, development, invasion, metastasis and prognosis. DDB1- and CUL4-associated factor 13 (DCAF13) is an RBPs has been identified as a substrate receptor for the CUL4-DDB1 E3 ligase complex. Its expression is related to the prognosis of certain cancer. We tried to explore both co-expressed network and biological functions of DCAF13 in breast cancer. Methods The Cancer Genome Atlas (TCGA) database was used to analyze the different expression of DCAF13 messenger RNA (mRNA) between normal breast tissue and breast carcinoma tissue, and the clinical data about 960 samples were downloaded from the cBio Cancer Genomics Portal (cBioPortal). The expression level of DCAF13, co-expression network, and survival were analyzed. Those with a fold change ≥1 and FDR <0.05 were considered to have statistical significance. Unsupervised clustering of differentially expressed RBPs was performed based on log2-transformed FPKM values using the “pheatmap” package in R. Genes with a Spearman score >0.55 were regarded as moderately co-expressed genes. The Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database was used to construct a co-expression network. Meanwhile, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to identify the biological process cluster and pathway cluster, respectively. Results Compared with normal breast tissue, DCAF13 mRNA expression was significantly increased in breast cancer tissue (P<0.01). The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to identify the functions of the co-expressed network. These genes were mainly enriched in mitosis, nuclear division, metabolic process, recombination, replication and repair of DNA, double-strand break repair, posttranscriptional regulation of gene expression, regulation of cell cycle, division and proliferation, regulation of protein stability and also participation in in regulation of poly(A) RNA binding, mRNA binding, tRNA binding, adenosine triphosphate (ATP) binding. KEGG pathway analysis revealed that the genes were mainly enriched in cell cycle, oocyte meiosis and oxidative phosphorylation. According to survival analysis, upregulation of DCAF13 mRNA was significant for overall survival (OS) (P=0.0163). Conclusions DCAF13 is up-regulated in breast cancer, the OS of patients with DCAF13 up-regulation was obviously reduced. DCAF13 was used as a diagnostic marker and therapeutic target for breast cancer. By building a co-expression network of DCAF13 and conducting bioinformatics analysis, it is possible to find the biomarker to...

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WW domain binding protein 2 (WBP2) as an oncogene in breast cancer: mechanisms and therapeutic prospects—a narrative review

Cited by 3 publications

References 99 publications

Variation of gene expression of fatty acid acyl CoA reductase associated with wax secretion of a scale insect, Ericerus pela, and identification of its regulation factors through the accessible chromatin analyses and yeast one‐hybrid

Variation of gene expression of fatty acid acyl CoA reductase associated with wax secretion of a scale insect, Ericerus pela, and identification of its regulation factors through the accessible chromatin analyses and yeast one‐hybrid

Identification of Novel Biomarkers in Peripheral Blood Mononuclear Cells of Hepatocellular Carcinoma Patients

Function and prognosis analysis of nucleolus protein DCAF13 in breast cancer

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