WU and KI polyomaviruses in respiratory, blood and urine samples from renal transplant patients

Csoma, Eszter; Mészáros, Beáta; Asztalos, László; Gergely, Lajos

doi:10.1016/j.jcv.2014.12.020

Cited by 16 publications

(19 citation statements)

References 24 publications

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“…BKPyV, JCPyV and TSPyV can be found in the kidney (Doerries, 2006;Fischer et al, 2012), whereas KIPyV, MCPyV, HPyV9 and STLPyV DNA can be detected in urine (Csoma et al, 2012(Csoma et al, , 2015Husseiny et al, 2010;Lim et al, 2013;Mertz et al, 2010;Rockett et al, 2013). The BKPyV, MCPyV and TSPyV early and late promoters were among the strongest in HEK293 cells.…”

Section: Discussionmentioning

confidence: 99%

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Moens

Ghelue

Ludvigsen

et al. 2015

Journal of General Virology

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Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines Little is known about cell tropism of the novel HPyVs, and cell cultures allowing virus propagation are lacking. Because viral tropism partially depends on the interaction of cellular transcription factors with the viral promoter, we monitored the promoter activity of all known HPyVs. Therefore, we compared the relative early and late promoter activity of the BK polyomavirus (BKPyV) (WW strain) with the corresponding activities of the other HPyVs in 10 different cell lines derived from brain, colon, kidney, liver, lung, the oral cavity and skin. Our results show that the BKPyV, MCPyV, TSPyV and HPyV12 early promoters displayed the strongest activity in most cell lines tested, while the remaining HPyV had relative low early promoter activity. HPyV12 showed the highest late promoter activity of all HPyVs in most cell lines, but also the BKPyV, MCPyV and TSPyV late promoters belonged to the stronger ones among HPyVs. The HPyVs with weak early promoter activity had in general also weak late promoter activity, except for HPyV10 whose late promoter was relatively strong in six of the 10 cell lines. A 20 bp deletion in the promoter of an HPyV12 variant significantly affected both early and late promoter activity in most cell lines. In conclusion, our findings suggest which cell lines may be suitable for virus propagation and may give an indication of the cell tropism of the HPyVs.

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Section: Discussionmentioning

confidence: 99%

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Moens

Ghelue

Ludvigsen

et al. 2015

Journal of General Virology

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“…To detect WU and KI virus DNA, nested and duplex real-time PCRs were carried out with the primers [58,59] and protocols detailed previously [39,40]. Plasmids containing the KIPyV and WUPyV genomes were used as positive controls kindly provided by Tobias Allander from Karolinska Institute (Stockholm, Sweden) and David Wang from Washington University (St. Louis, Miss., USA) [21,60].…”

Section: Methodsmentioning

confidence: 99%

“…Their respiratory transmission is suggested based on prevalence data from numerous studies, as has been summarized in review articles [19,37,38]. Both KI and WU viruses were detected in urine samples, which suggests that these viruses may also infect the urinary tract [39,40,41]. …”

Section: Introductionmentioning

confidence: 99%

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

Csoma¹,

Bidiga

Méhes

et al. 2016

Pathobiology

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Background/Aims: The oncogenic potential of human polyomaviruses (HPyVs) has been proposed, but so far only Merkel cell carcinoma polyomavirus seems to be associated with a human tumour. The role of BK polyomavirus (BKPyV) in human tumourigenesis remains controversial. BKPyV establishes persistent infection in the urinary tract, and renal and bladder neoplasms have been studied extensively, but conflicting prevalence data are reported. KI, WU and HPyV9 were detected in urine samples suggesting that these viruses may also infect the urinary tract, but their presence in urinary tract tumours has not been studied. The aim of this work was to examine the prevalence of KIPyV, WUPyV, HPyV9 and BKPyV by PCR in renal and bladder neoplasms. Methods: A total of 190 formalin-fixed paraffin-embedded renal neoplasms, bladder cancer and kidney biopsy samples were analysed for the presence of BKPyV, KIPyV, WUPyV and HPyV9 DNA by real-time and nested PCR. Results: Amplifiable DNA was extracted from all the samples, but none of the studied viruses were detected in benign renal neoplasia (0/23), malignant renal tumours (0/89) or bladder cancer (0/76). Conclusion: Our study did not find any evidence that BKPyV, KIPyV, WUPyV or HPyV9 are associated with bladder and renal tumours.

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“…Intra-assay analysis KIPyV 5 × 10 6 23.00 0.21 0.9 5 × 10 5 25.52 0.27 1.1 5 × 10 4 28.92 0.41 1.4 5 × 10 3 33.12 0.32 1 5 × 10 2 36.81 0.47 1.2 WUPyV 5 × 10 6 22.12 0.37 1.7 5 × 10 5 25.26 0.22 0.9 5 × 10 4 28.56 0.26 0.9 5 × 10 3 32.41 0.22 0.7 5 × 10 2 36.1 0.48 1.3…”

Section: Coefficient Of Variation (%)mentioning

confidence: 99%

“…KIPyV and WUPyV, as the BK and JC polyomaviruses, persist in a latent form in the human body but they could mainly reactivate in patients with immunosuppression. The initial identification of KIPyV and WUPyV in respiratory samples was progressively extended to other human fluids including blood, cerebrospinal fluid, lymphoid tissue, urine, stool and saliva [3][4][5][6]. Serological evaluations demonstrated that the primary infections by KIPyV and WUPyV occur early in life through the respiratory and fecal routes.…”

Section: Introductionmentioning

confidence: 99%

Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples

Ligozzi

Galia

Carelli

et al. 2018

Molecular and Cellular Probes

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In this study, we describe a duplex real-time PCR assay for the simultaneous detection of KIPyV and WUPyV polyomaviruses based on TaqMan probes. This assay detected 500 copies/mL both for KIPyV and WUPyV in 100% of tested positive samples. We assessed this technique on 482 nasopharyngeal aspirate specimens from hospitalized pediatric patients with respiratory symptoms, previously analyzed with commercial multiplex assay for 16 major respiratory viruses. Our assay detected KIPyV genome in 15 out of 482 samples (3.1%) and WUPyV genome in 24 out of 482 samples (4.9%), respectively, and in three samples the coinfection of the two viruses was found. Interestingly, 29 out of 36 of samples with KIPyV and/or WUPyV infection exhibited a co-infection with one or more respiratory viruses confirming that KIPyV and WUPyV were often detected in association to other viral infections. Of note, KIPyV and WUPyV were detected singularly in 4 out of 15 cases and 3 out of 24 cases, respectively, suggesting a possible direct role of these viruses in the respiratory diseases. In conclusion, this method could be taken into account as an alternative technical approach to detect KIPyV and/or WUPyV in respiratory samples for epidemiological and diagnostic analyses.

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WU and KI polyomaviruses in respiratory, blood and urine samples from renal transplant patients

Abstract: 21Background: It is suggested that immunosuppression due to transplantation might be a risk 22

Cited by 16 publications

References 24 publications

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples

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