WT1 and its transcriptional cofactor BASP1 redirect the differentiation pathway of an established blood cell line

Goodfellow, Sarah J.; Rebello, Michelle R.; Toska, Eneda; Zeef, Leo; Rudd, Sean G.; Medler, Kathryn F.; Roberts, Stefan G. E.

doi:10.1042/bj20101734

Cited by 39 publications

(66 citation statements)

References 43 publications

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“…6d) (Rege and Hagood, 2006), and INA (FC 89) (Baum and Garriga, 1997). Additional down-regulated genes were involved in neuron differentiation and stabilization such as FERD3L (FC 104) (Ono et al , 2010), BASP1 (FC 200) (Goodfellow et al , 2011), and HSPA12B (FC 119) (Ono et al , 2010). Finally, other highly down-regulated genes played a role in cell proliferation ( MZF1 , FC 124) (Gaboli et al , 2001); DNA damage repair ( XRCC3 , FC 121) (Kurumizaka et al , 2001); and protein transport ( SSR4 , FC 183) (Nagasawa et al , 2007) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Robust gene expression changes in the ganglia following subclinical reactivation in rhesus macaques infected with simian varicella virus

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Robust gene expression changes in the ganglia following subclinical reactivation in rhesus macaques infected with simian varicella virus

et al. 2017

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“…Several of these (BASP1/MARCKS/MARCKSL1) interact, and although not known as direct interactors of β-catenin, may function (or interact) in concert with β-catenin as shown in Figure 6. BASP1 is a well-characterized interactor of WT1/Wilms Tumor 1, functioning as a transcriptional cosuppressor (38,39). For example, FAM123B/WTX (Wilms tumor on the X) is a WT1 associated protein, that also directly interacts with APC and β-catenin (40,41).…”

Section: Resultsmentioning

confidence: 99%

Integrated analysis of the Wnt responsive proteome in human cells reveals diverse and cell-type specific networks

Song

Wang²,

Ewing

2014

Mol. BioSyst.

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Wnt signalling is a fundamentally important signalling pathway that regulates many aspects of metazoan development and is frequently dysregulated in cancer. Although many of the core components of the Wnt signalling pathway, such as β-catenin, have been extensively studied, the broad systems level responses of the mammalian cell to Wnt signalling are less well understood. In addition, the cell- or tissue-specific protein networks that modulate Wnt signalling in the diverse tissues or developmental stages in which it functions remain to be defined. To address these questions, we undertook a broad survey of the Wnt response in different human cell lines using both interaction and expression proteomics approaches. Our data reveal both similar and divergent responses of pathways and processes in the three cell-lines analyzed as well as a marked attenuation of the response to exogenous Wnt treatment in cells harbouring a stabilizing (activating) mutation of β-catenin. We also identify cell-type specific components of the Wnt signalling network and find that by integrating expression and interaction proteomics data a more complete description of the Wnt interaction network can be achieved. Finally, our results attest to the power of LCMS/MS to reveal novel cellular responses in even relatively well studied biological pathways such as Wnt signalling.

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“…We note, however, that LEF1 expression is not evident in all the regions of the placode that express WT1, suggesting that other factors are also important. Indeed, we and others have previously shown that the function of WT1 in transcriptional activation is modulated by its interaction with BASP1 and that this is both cell type-and gene promoter-specific (Essafi et al, 2011;Goodfellow et al, 2011;Green et al, 2009). It will therefore be important to study the potential role of BASP1 in modulating WT1 function during CV formation.…”

Section: Discussionmentioning

confidence: 99%

WT1 regulates the development of the posterior taste field

et al. 2014

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Despite the importance of taste in determining nutrient intake, our understanding of the processes that control the development of the peripheral taste system is lacking. Several early regulators of taste development have been identified, including sonic hedgehog, bone morphogenetic protein 4 and multiple members of the Wnt/β-catenin signaling pathway. However, the regulation of these factors, including their induction, remains poorly understood. Here, we identify a crucial role for the Wilms' tumor 1 protein (WT1) in circumvallate (CV) papillae development. WT1 is a transcription factor that is important in the normal development of multiple tissues, including both the olfactory and visual systems. In mice, WT1 expression is detectable by E12.5, when the CV taste placode begins to form. In mice lacking WT1, the CV fails to develop normally and markers of early taste development are dysregulated compared with wild type. We demonstrate that expression of the WT1 target genes Lef1, Ptch1 and Bmp4 is significantly reduced in developing tongue tissue derived from Wt1 knockout mice and that, in normal tongue, WT1 is bound to the promoter regions of these genes. Moreover, siRNA knockdown of WT1 in cultured taste cells leads to a reduction in the expression of Lef1 and Ptch1. Our data identify WT1 as a crucial transcription factor in the development of the CV through the regulation of multiple signaling pathways that have established roles in the formation and patterning of taste placodes.

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WT1 and its transcriptional cofactor BASP1 redirect the differentiation pathway of an established blood cell line

Cited by 39 publications

References 43 publications

Robust gene expression changes in the ganglia following subclinical reactivation in rhesus macaques infected with simian varicella virus

Robust gene expression changes in the ganglia following subclinical reactivation in rhesus macaques infected with simian varicella virus

Integrated analysis of the Wnt responsive proteome in human cells reveals diverse and cell-type specific networks

WT1 regulates the development of the posterior taste field

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