WSV181 inhibits JAK/STAT signaling and promotes viral replication in Drosophila

Wang, Wei; Pan, Changkun; Huang, Zhenpeng; Yuan, Huifang; Chen, Jianming

doi:10.1016/j.dci.2018.11.003

Cited by 9 publications

(9 citation statements)

References 60 publications

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“…[26][27][28]38,39 However, there is still little evidence defining the role of LINC00511 in EMT. [26][27][28]38,39 However, there is still little evidence defining the role of LINC00511 in EMT.…”

Section: Discussionmentioning

confidence: 99%

LINC00511 contributes to glioblastoma tumorigenesis and epithelial‐mesenchymal transition via LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop

Liu

et al. 2019

J Cellular Molecular Medi

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Tumour invasion is closely related to the prognosis and recurrence of glioblastoma multiforme and partially attributes to epithelial‐mesenchymal transition. Long intergenic non‐coding RNA 00511 (LINC00511) plays a pivotal role in tumour; however, the role of LINC00511 in GBM, especially in the epigenetic molecular regulation mechanism of EMT, is still unclear. Here, we found that LINC00511 was up‐regulated in GBM tissues and relatively high LINC00511 expression predicted poorer prognosis. Moreover, ectopic LINC00511 enhanced GBM cells proliferation, EMT, migration and invasion, whereas LINC00511 knockdown had the opposite effects. Mechanistically, we confirmed that ZEB1 acted as a transcription factor for LINC00511 in GBM cells. Subsequently, we found that LINC00511 served as a competing endogenous RNA that sponged miR‐524‐5p to indirectly regulate YB1, whereas, up‐regulated YB1 promoted ZEB1 expression, which inversely facilitated LINC00511 expression. Finally, orthotopic xenograft models were performed to further demonstrate the LINC00511 on GBM tumorigenesis. This study demonstrates that a LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop provides potential therapeutic targets for GBM progression.

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Section: Discussionmentioning

confidence: 99%

LINC00511 contributes to glioblastoma tumorigenesis and epithelial‐mesenchymal transition via LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop

Liu

et al. 2019

J Cellular Molecular Medi

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“…Interestingly, by comparing sterile-bred and conventionally bred animals, Buchon et al (2009a) demonstrated that contact between conventional microorganisms and commensal bacteria can also activate basal levels of Upd expression and subsequent intestinal epithelium renewal at a lower rate through the JAK/STAT pathway (Lee, 2009). Recent studies have found that the JAK/STAT pathway plays a vital role in the host defense against viral infection (Souza-Neto et al, 2009;Morin-Poulard et al, 2014;Wang et al, 2019b). In addition, Upd3 production and JAK/STAT pathway activation in the intestine must also depend on the oxidative burst of Duox (Royet, 2011).…”

Section: Role Of the Jak/stat Signaling Pathway On Intestinal Microor...mentioning

confidence: 99%

Regulatory mechanisms of microbial homeostasis in insect gut

et al. 2020

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Insects live in incredibly complex environments. The intestinal epithelium of insects is in constant contact with microorganisms, some of which are beneficial and some harmful to the host. Insect gut health and function are maintained through multidimensional mechanisms that can proficiently remove foreign pathogenic microorganisms while effectively maintaining local symbiotic microbial homeostasis. The basic immune mechanisms of the insect gut, such as the dual oxidase-reactive oxygen species (Duox-ROS) system and the immune deficiency (Imd)-signaling pathway, are involved in the maintenance of microbial homeostasis. This paper reviews the role of physical defenses, the Duox-ROS and Imd signaling pathways, the Janus kinase/signal transducers and activators of transcription signaling pathway, and intestinal symbiotic flora in the homeostatic maintenance of the insect gut microbiome.

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“…vannamei , resulted in less WSSV infection 50 . More recently, Wang, W. et al ., 2019 reported that inhibition of the JAK/STAT pathway in Drosophila by WSV181 could enhance WSSV replication 51 . These findings indicated that the JAK/STAT pathway could function as anti-viral response.…”

Section: Discussionmentioning

confidence: 99%

Role of Clathrin Assembly Protein-2 Beta Subunit during White Spot Syndrome Virus Infection in Black Tiger Shrimp Penaeus monodon

Jatuyosporn

Laohawutthichai

Supungul

et al. 2019

Sci Rep

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White spot syndrome virus (WSSV) is one of the most lethal viruses severely affecting shrimp industry. This disease can cause 100% mortality of farmed shrimp within a week. This work aims to characterize clathrin assembly proteins in Penaeus monodon and investigate their roles in WSSV entry. In general, clathrin assembly proteins form complexes with specific receptors and clathrins, leading to clathrin-mediated endocytosis. Adaptor protein 2 (AP-2), which is responsible for endocytosis at plasma membrane, consists of four subunits including α, β2, μ2 and σ2. Knockdown of clathrin coat AP17, or σ subunit of AP-2 dramatically reduced WSSV infectivity. Similar results were observed, when shrimp were pre-treated with chlorpromazine (CPZ), an inhibitor of clathrin-dependent endocytosis. The complete open reading frames of AP-2β and μ subunits of P. monodon are reported. PmAP-2 β was up-regulated about 4-fold at 6 and 36 h post-WSSV infection. Knockdown of PmAP-2β delayed shrimp mortality during WSSV infection, of which WSSV intermediate early 1 gene expression was also down-regulated. Immunogold-labelling and transmission electron microscopy revealed that PmAP-2β co-localized with WSSV particles at plasma membrane. In addition, PmAP-2β-silencing significantly affected the expression levels of PmSTAT, PmDOME, PmDorsal and ALFPm3 during WSSV infection. It is possible that PmAP-2β is associated with the JAK/STAT and the Toll pathway.

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WSV181 inhibits JAK/STAT signaling and promotes viral replication in Drosophila

Cited by 9 publications

References 60 publications

LINC00511 contributes to glioblastoma tumorigenesis and epithelial‐mesenchymal transition via LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop

LINC00511 contributes to glioblastoma tumorigenesis and epithelial‐mesenchymal transition via LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop

Regulatory mechanisms of microbial homeostasis in insect gut

Role of Clathrin Assembly Protein-2 Beta Subunit during White Spot Syndrome Virus Infection in Black Tiger Shrimp Penaeus monodon

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