WSSV-responsive gene expression under the influence of PmVRP15 suppression

Tummamunkong, P.; Jaree, Phattarunda; Tassanakajon, Anchalee; Somboonwiwat, Kunlaya

doi:10.1016/j.fsi.2017.10.006

Cited by 1 publication

(1 citation statement)

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“…The shrimps were fed with commercial pellets 4 times a day during the course of the experiments. The shrimps were sampled to determine if they were free from VP AHPND and WSSV-infections by PCR prior to experiments using the specific primer as described by Sirikharin et al 35 and Tummamunkong et al 36 (Fig. S2).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus

Boonchuen

Maralit

Jaree

et al. 2020

Sci Rep

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While Vibrio parahaemolyticus (Vp AHpnD) has been identified as the cause of early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in shrimp, mechanisms of host response remain unknown. Understanding these processes is important to improve farming practices because this understanding will help to develop methods to enhance shrimp immunity. Pre-treatment of shrimp with 5-minute chronic non-lethal heat stress (NLHS) for 7 days was found to significantly increase Litopenaeus vannamei survival against Vp AHpnD infection. To elucidate the mechanism involved, mRNA and miRNA expression profiles from the hemocyte of L. vannamei challenged with Vp AHpnD after NLHS with corresponding control conditions were determined by RNA-Seq. A total of 2,664 mRNAs and 41 miRNAs were differentially expressed after the NLHS treatment and VP AHpnD challenge. A miRnA-mRNA regulatory network of differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) was subsequently constructed and the interactions of DEMs in regulating the NLHSinduced immune-related pathways were identified. Transcriptomic data revealed that miRNA and mRNA interactions contribute to the modulation of NLHS-induced immune responses, such as the prophenoloxidase-activating system, hemocyte homeostasis, and antimicrobial peptide production, and these responses enhance Vp AHpnD resistance in L. vannamei. During its first outbreak, the devastating effect of early mortality syndrome (EMS) on the global shrimp industry was primarily caused by the lack of information regarding the disease and its causative agent 1. Later studies that were focused on mitigating this disease eventually identified the cause to be a toxin-harboring Vibrio parahaemolyticus, which causes Acute hepatopancreatic necrosis disease (AHPND) (VP AHPND) 2. This highlights how understanding disease etiology and a subsequent elucidation of host response can help to mitigate the effects of an outbreak. Understanding the functions of the shrimp immune system during disease progression is thus expected to create opportunities for the development of effective and efficient management strategies for VP AHPND infection. Likewise, information regarding the molecular mechanisms of AHPND tolerance can lead to platforms for the development of AHPND-resistant shrimp through selective breeding using markers mined from transcriptome analyses at different rearing conditions.

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Section: Methodsmentioning

confidence: 99%