WRKY33 interacts with WRKY12 protein to up‐regulate RAP2.2 during submergence induced hypoxia response in Arabidopsis thaliana

Tang, Hu; Bi, Hao; Liu, Bao; Lou, Shangling; Song, Yan; Tong, Shaofei; Chen, Ningning; Jiang, Yuanzhong; Liu, Jianquan; Liu, Huanhuan

doi:10.1111/nph.17020

Cited by 86 publications

(75 citation statements)

References 63 publications

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“…Recently, WRKY33 was reported to positively regulate submergence response via interacting with WRKY12 to directly upregulate RAP2.2 in Arabidopsis [17]. To further identify other WRKY33 targeted genes during submergence response at a genome-wide scale, we aimed to conduct the chromatin immunoprecipitation followed by high throughput sequencing (ChIP-Seq) using 35S:FLAG-WRKY33 overexpression transgenic plants (WRKY33-OE) upon 24 hours' submergence treatment.…”

Section: Resultsmentioning

confidence: 99%

“…The identi ed "TC box" cis-element is a new motif different from the known "W box" element for WRKY33 and may be speci c to regulating the target genes during submergence stress. WRKY33 can regulate RAP2.2 expression via the W box element only during the submergence response [17]. Interestingly, there also is a "TC box" sequence "TCTCTC" in the promoter region (-1,875 bp) of RAP2.2.…”

Section: Discussionmentioning

confidence: 99%

“…This switch is mediated by the phosphorylation in IPA1 protein. Submergence treatment might also can induce the phosphorylation of WRKY33 like IPA1 upon pathogen infection [17,22]. In addition, this TF may also have different binding abilities towards "W box" or "TC box" elements between normal growth and submergence treatment conditions like IPA1.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a recent study has revealed that WRKY33 positively regulates submergence-induced hypoxia in Arabidopsis thaliana (L.) Heynh. and directly acts on the RAP2.2 gene, which encodes a TF belonging to the group VII Ethylene Response Factors (ERFVIIs) [17]. Despite these studies, the downstream regulatory network governed by WRKY33 is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide (ChIP-seq) Identification of Target Genes Regulated by WRKY33 During Submergence Stress in Arabidopsis

Zhang

Liu

Song

et al. 2020

Preprint

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Background: Hypoxia induced by flooding causes significant losses to crop production almost every year. However, the molecular network of hypoxia signaling pathway is still poorly understood. According to previous studies, transgenic plants overexpressing the WRKY33 gene showed enhanced resistance to submergence stress. Thus, the transcription factor WRKY33 may regulate a series of target genes in response to submergence. Here, to determine the putative downstream targets of WRKY33 at a genome-wide scale in Arabidopsis thaliana, we performed the chromatin immunoprecipitation sequencing (ChIP-seq) using 35S:FLAG-WRKY33 overexpression transgenic lines (WRKY33-OE) upon 24 hours of submergence treatment.Results: Using ChIP-seq data, we identified a total of 104 WRKY33-binding genes under submergence treatment (WRKY33BGHs). Most of the WRKY33BGHs are involved in the oxidation-reduction process, programmed cell death in response to reactive oxygen species, lipid biosynthesis process, and other processes related to stress responses. Moreover, the major motif identified in the WRKY33BGHs promoters is a new cis-element, TCTCTC (we named it as “TC box”). This cis-element is different from the previously known W box for WRKY33. Further qPCR experiments verified that genes carrying this motif in their promoters could be regulated by WRKY33 upon submergence treatment.Conclusions: Our study has identified a new putative binding motif of WRKY33 and recovered numerous previously unknown target genes of WRKY33 during submergence stress. The WRKY33 gene positively participates in flooding response probably by transcriptional regulation of the downstream hypoxia-related target genes via a “TC box”.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide (ChIP-seq) Identification of Target Genes Regulated by WRKY33 During Submergence Stress in Arabidopsis

Zhang

Liu

Song

et al. 2020

Preprint

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“…Additionally, AtWRKY33 plays a critical role in widespread stress responses. The wrky33 mutant is sensitive to NaCl stress (Jiang and Deyholos, 2009), and WRKY33 can bind to RAP2.2 to regulate its expression during submergence induced hypoxia response (Tang et al, 2020). Overexpression of AtWRKY33 enhances plants resistance against the necrotrophic fungal pathogen Botrytis cinerea (Birkenbihl et al, 2012; Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

BnaA03.WRKY28, interacting with BnaA09.VQ12, acts as a brake factor of activated BnWRKY33-mediated resistance outburst againstSclerotinia sclerotioruminBrassica napus

Zhang

Liu

et al. 2021

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Sclerotinia sclerotiorum causes substantial damage to the growth of Brassica napus (rapeseed) and makes a significant loss of crop yield. The plant innate immune system may be the primary solution to defense against S. sclerotiorum for rapeseed. Here, we identify that BnWRKY33, a transcription factor in the innate immune pathway, can be rapidly phosphorylated and activated by the MAPK cascade after rapeseed is infected with S. sclerotiorum. In the MAPK cascade, activated BnaA03.MKK4 phosphorylates and activates BnaA06.MPK3 and BnaC03.MPK3. The activated BnMPK3 acts on the substrate BnWRKY33 to enhance its transcriptional activity and trigger a transcriptional burst of BnWRKY33, which helps plants effectively resist the pathogenic fungi by enhancing the expression of phytoalexin synthesis-related genes. With constant infection, BnaA03.WRKY28 and BnaA09.VQ12 are induced, and BnaA03.WRKY28 physically interacts with BnaA09.VQ12 to form a protein complex. BnaA03.WRKY28 preferentially binds to the promoter of BnWRKY33 with the help of BnaA09.VQ12. Compared with activated BnWRKY33, BnaA03.WRKY28 has a lower transcriptional activity on downstream BnWRKY33, which leads to weaker resistance against S. sclerotiorum for rapeseed in the later stage of infection. Furthermore, the induced BnaA03.WRKY28 may promote axillary bud activity and axillary meristem initiation by regulating the expression of branching-related genes (such as BnBRC1), thus promoting the formation of branches in the leaf axils.One-sentence summaryUnder constant infection by Sclerotinia sclerotiorum, BnaA03.WRKY28 interacts with BnaA09.VQ12 and takes precedence over phosphorylated BnWRKY33 to bind to the BnWRKY33 promoter, thereby weakening resistance but promoting branching.

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Plant responses to limited aeration: Advances and future challenges

et al. 2023

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Limited aeration that is caused by tissue geometry, diffusion barriers, high elevation, or a flooding event poses major challenges to plants and is often, but not exclusively, associated with low oxygen. These processes span a broad interest in the research community ranging from whole plant and crop responses, post‐harvest physiology, plant morphology and anatomy, fermentative metabolism, plant developmental processes, oxygen sensing by ERF‐VIIs, gene expression profiles, the gaseous hormone ethylene, and O2 dynamics at cellular resolution. The International Society for Plant Anaerobiosis (ISPA) gathers researchers from all over the world contributing to understand the causes, responses, and consequences of limited aeration in plants. During the 14th ISPA meeting, major research progress was related to the evolution of O2 sensing mechanisms and the intricate network that balances low O2 signaling. Here, the work moved beyond flooding stress and emphasized novel underexplored roles of low O2 and limited aeration in altitude adaptation, fruit development and storage, and the vegetative development of growth apices. Regarding tolerance towards flooding, the meeting stressed the relevance and regulation of developmental plasticity, aerenchyma, and barrier formation to improve internal aeration. Additional newly explored flood tolerance traits concerned resource balance, senescence, and the exploration of natural genetic variation for novel tolerance loci. In this report, we summarize and synthesize the major progress and future challenges for low O2 and aeration research presented at the conference.

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WRKY33 interacts with WRKY12 protein to up‐regulate RAP2.2 during submergence induced hypoxia response in Arabidopsis thaliana

Cited by 86 publications

References 63 publications

Genome-wide (ChIP-seq) Identification of Target Genes Regulated by WRKY33 During Submergence Stress in Arabidopsis

Genome-wide (ChIP-seq) Identification of Target Genes Regulated by WRKY33 During Submergence Stress in Arabidopsis

BnaA03.WRKY28, interacting with BnaA09.VQ12, acts as a brake factor of activated BnWRKY33-mediated resistance outburst againstSclerotinia sclerotioruminBrassica napus

Plant responses to limited aeration: Advances and future challenges

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