“…In addition, only MsZFP11 and MsZFP13 had DRE regulatory elements, while MsZFP58 did not have any cis -regulatory elements among the investigated regulatory elements. A previous study confirmed that many important cis -regulatory elements already existed in the promoter regions of stress response genes in MYBs, WRKYs, and MADS-boxes [ 35 , 40 , 46 ]. Furthermore, many of these elements, such as ABREs and DREs, have been reported to widely participate in abiotic stress responses in Artemisia annua , Arabidopsis and wheat [ 47 , 48 , 49 ].…”
Section: Discussionmentioning
confidence: 58%
“…Every 10 μL reaction volume consisted of 5 μL of 2 × SG Fast qPCR Master Mix, 1 μL of cDNA, 1 μL of DNF buffer, 0.2 μL of each of the forward and reverse primer at 10 μM, and 2.6 μL of sterilized ddH 2 O. The reaction process was set as follows: denaturation (95 ℃/30 s) and 40 cycles at 95 °C /5 s and 60 °C /30 s. Every reaction consisted of three replicates, and according to the expression of the Medicago actin gene (AA660796) [ 35 ], the relative expression level of MsZFP genes was normalized with the 2 −ΔΔCq method. According to the genome data of XinJiangDaYe alfalfa, all MsZFP gene primer pairs were designed using Primer 6 software; the lengths of the PCR products are listed in Table S1 .…”
Q-type C2H2 zinc-finger protein (C2H2-ZFP) transcription factors are associated with many plant growth development and environmental stress responses. To date, there have been few analyses of the Q-type C2H2-ZFP gene family in alfalfa (Medicago sativa subsp. sativa). In this study, we identified 58 Q-type C2H2-ZFPs across the entire alfalfa genome, and the gene structure, motif composition, chromosomal mapping, and cis-regulatory elements were explored, as well as the expression profiles of specific tissues and the response under different abiotic stresses. According to their phylogenetic features, these 58 MsZFPs were divided into 12 subgroups. Synteny analysis showed that duplication events play a vital role in the expansion of the MsZFP gene family. The collinearity results showed that a total of 26 and 42 of the 58 MsZFP genes were homologous with Arabidopsis and M. truncatula, respectively. The expression profiles showed that C2H2-ZFP genes played various roles in different tissues and abiotic stresses. The results of subsequent quantitative real-time polymerase chain reaction (qRT-PCR) showed that the nine selected MsZFP genes were rapidly induced under different abiotic stresses, indicating that C2H2-ZFP genes are closely related to abiotic stress. This study provides results on MsZFP genes, their response to various abiotic stresses, and new information on the C2H2 family in alfalfa.
“…In addition, only MsZFP11 and MsZFP13 had DRE regulatory elements, while MsZFP58 did not have any cis -regulatory elements among the investigated regulatory elements. A previous study confirmed that many important cis -regulatory elements already existed in the promoter regions of stress response genes in MYBs, WRKYs, and MADS-boxes [ 35 , 40 , 46 ]. Furthermore, many of these elements, such as ABREs and DREs, have been reported to widely participate in abiotic stress responses in Artemisia annua , Arabidopsis and wheat [ 47 , 48 , 49 ].…”
Section: Discussionmentioning
confidence: 58%
“…Every 10 μL reaction volume consisted of 5 μL of 2 × SG Fast qPCR Master Mix, 1 μL of cDNA, 1 μL of DNF buffer, 0.2 μL of each of the forward and reverse primer at 10 μM, and 2.6 μL of sterilized ddH 2 O. The reaction process was set as follows: denaturation (95 ℃/30 s) and 40 cycles at 95 °C /5 s and 60 °C /30 s. Every reaction consisted of three replicates, and according to the expression of the Medicago actin gene (AA660796) [ 35 ], the relative expression level of MsZFP genes was normalized with the 2 −ΔΔCq method. According to the genome data of XinJiangDaYe alfalfa, all MsZFP gene primer pairs were designed using Primer 6 software; the lengths of the PCR products are listed in Table S1 .…”
Q-type C2H2 zinc-finger protein (C2H2-ZFP) transcription factors are associated with many plant growth development and environmental stress responses. To date, there have been few analyses of the Q-type C2H2-ZFP gene family in alfalfa (Medicago sativa subsp. sativa). In this study, we identified 58 Q-type C2H2-ZFPs across the entire alfalfa genome, and the gene structure, motif composition, chromosomal mapping, and cis-regulatory elements were explored, as well as the expression profiles of specific tissues and the response under different abiotic stresses. According to their phylogenetic features, these 58 MsZFPs were divided into 12 subgroups. Synteny analysis showed that duplication events play a vital role in the expansion of the MsZFP gene family. The collinearity results showed that a total of 26 and 42 of the 58 MsZFP genes were homologous with Arabidopsis and M. truncatula, respectively. The expression profiles showed that C2H2-ZFP genes played various roles in different tissues and abiotic stresses. The results of subsequent quantitative real-time polymerase chain reaction (qRT-PCR) showed that the nine selected MsZFP genes were rapidly induced under different abiotic stresses, indicating that C2H2-ZFP genes are closely related to abiotic stress. This study provides results on MsZFP genes, their response to various abiotic stresses, and new information on the C2H2 family in alfalfa.
“…WRKYs are plant-specific transcription factors (TFs) and have also been found in protozoans (Giardia lamblia) and amoeboid (Dictyostelium discoideum), indicating a long evolutionary history (Goyal et al, 2020;Mao et al, 2020). The DNA-binding domain of WRKY TFs is 60 amino acids long and has a highly conserved heptapeptide (WRKYGQK) signature motif on the N-terminus and a zinc finger-like motif on the Cterminus (Eulgem et al, 2000).…”
WRKY transcription factors (TFs), one of the largest TF families, serve critical roles in the regulation of secondary metabolite production. However, little is known about the expression pattern of WRKY genes during the germination and maturation processes of Toona sinensis buds. In the present study, the new assembly of the T. sinensis genome was used for the identification of 78 TsWRKY genes, including gene structures, phylogenetic features, chromosomal locations, conserved protein domains, cis-regulatory elements, synteny, and expression profiles. Gene duplication analysis revealed that gene tandem and segmental duplication events drove the expansion of the TsWRKYs family, with the latter playing a key role in the creation of new TsWRKY genes. The synteny and evolutionary constraint analyses of the WRKY proteins among T. sinensis and several distinct species provided more detailed evidence of gene evolution for TsWRKYs. Besides, the expression patterns and co-expression network analysis show TsWRKYs may multi-genes co-participate in regulating terpenoid biosynthesis. The findings revealed that TsWRKYs potentially play a regulatory role in secondary metabolite synthesis, forming the basis for further functional characterization of WRKY genes with the intention of improving T. sinensis.
“…Normal plant growth is often affected by a variety of adverse environmental factors such as drought, high salinity, temperature extremes and other abiotic stresses [ 1 ]. Abiotic stress inhibits normal plant growth, development, and function by speeding up chlorophyll degradation, disrupting chloroplast membrane activities, and decreasing photosynthetic efficiency, as shown by a number of studies [ 2 , 3 , 4 ]. In response to external stimuli, plants’ bodies produce signals that trigger the phosphorylation of downstream proteins, which in turn trigger a number of transcription factors [ 5 ].…”
Alfalfa (Medicago sativa) is one of the most important legume forage species in the world. It is often affected by several abiotic stressors that result in reduced yields and poor growth. Therefore, it is crucial to study the resistance of M. sativa to abiotic stresses. Heat shock transcription factors (HSF) are key players in a number of transcriptional regulatory pathways. These pathways play an essential role in controlling how plants react to different abiotic stressors. Studies on the HSF gene family have been reported in many species but have not yet undergone a thorough analysis in M. sativa. Therefore, in order to identify a more comprehensive set of HSF genes, from the genomic data, we identified 16 members of the MsHSF gene, which were unevenly distributed over six chromosomes. We also looked at their gene architectures and protein motifs, and phylogenetic analysis allowed us to divide them into 3 groups with a total of 15 subgroups. Along with these aspects, we then examined the physicochemical properties, subcellular localization, synteny analysis, GO annotation and enrichment, and protein interaction networks of amino acids. Finally, the analysis of 16 MsHSF genes’ expression levels across all tissues and under four abiotic stresses using publicly available RNA-Seq data revealed that these genes had significant tissue-specific expression. Moreover, the expression of most MsHSF genes increased dramatically under abiotic stress, further validating the critical function played by the MsHSF gene family in abiotic stress. These results provided basic information about MsHSF gene family and laid a foundation for further study on the biological role of MsHSF gene in response to stress in M. sativa.
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