WRKY transcription factors and plant defense responses: latest discoveries and future prospects

Wani, Shabir Hussain; Anand, Sury; Singh, Balwant; Bohra, Abhishek; Joshi, Rohit

doi:10.1007/s00299-021-02691-8

Cited by 244 publications

(106 citation statements)

References 159 publications

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“…Transcriptional reprogramming in activation of immune response requires concerted and fine-tuned temporal and spatial functions of numerous transcription factors (TFs) belonging to different families (Buscaill and Rivas, 2014;Birkenbihl et al, 2017;Ng et al, 2018). During the last two decades, extensive genetic and biochemical studies have revealed the importance of TFs belonging to families of WRKY, AP2/ERF, NAC (NAM, ATAF1/2, and CUC2), MYB, bZIP, homeodomain, bHLH, NF-Y, and CAMTA in plant immunity (Nuruzzaman et al, 2013;Buscaill and Rivas, 2014;Dey and Vlot, 2015;Huang et al, 2016;Noman et al, 2017;Ng et al, 2018;Yuan et al, 2019a;Bian et al, 2020;Wani et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

Yuan

Wang

et al. 2021

Front. Plant Sci.

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NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of ONAC066 in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. ONAC066-OE plants exhibited enhanced resistance while ONAC066-Ri and onac066-1 plants showed attenuated resistance to Magnaporthe oryzae. A total of 81 genes were found to be up-regulated in ONAC066-OE plants, and 26 of them were predicted to be induced by M. oryzae. Four OsWRKY genes, including OsWRKY45 and OsWRKY62, were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to NAC core-binding site in OsWRKY62 promoter and activated OsWRKY62 expression, indicating that OsWRKY62 is a ONAC066 target. A set of cytochrome P450 genes were found to be co-expressed with ONAC066 and 5 of them were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to promoters of cytochrome P450 genes LOC_Os02g30110, LOC_Os06g37300, and LOC_Os02g36150 and activated their transcription, indicating that these three cytochrome P450 genes are ONAC066 targets. These results suggest that ONAC066, as a transcription activator, positively contributes to rice immunity through modulating the expression of OsWRKY62 and a set of cytochrome P450 genes to activate defense response.

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Section: Introductionmentioning

confidence: 99%

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

Yuan

Wang

et al. 2021

Front. Plant Sci.

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“…WRKY family genes are responsible for very important functions such as development stage, defense system, and signaling in plants [44]. Therefore, OsWRKY34q1 screened in this study can be effectively used to develop BLB resistant varieties.…”

Section: Discussionmentioning

confidence: 94%

Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field

Park

Kim

et al. 2021

Agronomy

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Rapid climate change has increased the incidence of various pests and diseases, and these threaten global food security. In particular, BLB (bacterial leaf blight) is caused by Xoo (Xanthomonas oryzae pv. oryzae) and its main characteristic is that the rice suddenly dries and withers. Recently, omics have been effectively used in agriculture. In particular, it is a key technology that can accurately diagnose diseases in the field. Until now, QTL (quantitative trait loci) mapping has been analyzed using only subjective phenotypic data by experts. However, in this study, diseases were accurately diagnosed using OCT (optical coherence tomography), and QTL mapping was performed using leaf thickness and leaf angles after Xoo inoculation. After Xoo inoculation of a 120 Cheongcheong/Nagdong double haploid (CNDH) population, QTL mapping was performed using the changing leaf angle, and OsWRKY34q1 was detected in RM811-RM14323 of chromosome 1. OsWRKY34q1 always had a higher expression level in the BLB-resistant population than in the susceptible population after Xoo inoculation. OsWRKY34q1 belongs to the WRKY family of genes. OsWRKY34q1 could be effectively used to develop BLB-resistant rice varieties in response to the current era of unpredictable climate change.

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“…Out of 71 genes of AP2/ERF TFfamilies, sixteen VrDREB genes were significantly upregulated under drought stress, and proved that these genes participate in pathways leading to drought tolerance in mungbean. Similarly, WRKY TFs also have great significance acting as positive, as well as negative regulators of stress responses [172]. Srivastava et al [173] identified 84 VaWRKY genes and 85 VrWRKY genes in adzuki bean and mungbean, respectively.…”

Section: Exploring Gene Families and Transcriptional Factors As Drought-responsive Markersmentioning

confidence: 99%

Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

Singh

Tiwari³

et al. 2021

Agronomy

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Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.

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WRKY transcription factors and plant defense responses: latest discoveries and future prospects

Cited by 244 publications

References 159 publications

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field

Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

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