Wound Therapy by Marrow Mesenchymal Cell Transplantation

Yoshikawa, Takafumi; Mitsuno, Hiroya; Nonaka, Iehisa; Sen, Yasunori; Kawanishi, K; Inada, Yûji; Takakura, Yoshinori; Okuchi, Kazuo; Nonomura, Akitaka

doi:10.1097/01.prs.0000299922.96006.24

Cited by 261 publications

(183 citation statements)

References 33 publications

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“…[25][26][27] However, only a small number have been tested in humans and consist of MSC incorporated into a fibrin spray 10 or collagen sponge. 11 Dermal substitute of animal origin may induce inflammatory reactions. 28 Therefore, further developments are warranted to lead to more clinical studies on tissue-engineered products.…”

Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

Roux

Bodivit

Bartis

et al. 2015

Tissue Engineering Part A

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Stem cells may represent an excellent strategy to improve the healing of skin ulcers. Today the administration mode of stem cells to skin defects remains unsatisfactory. Delivering stem cells with topical treatments represents a new strategy and answering the patients' need. Mesenchymal stromal cells (MSC) have been shown to improve wound healing of cutaneous lesions and amniotic membrane (AM) is known to represent a natural scaffold for cells. The aim of this study is to develop a tissue-engineered product combining MSC and AM for clinical use. In this work we investigated whether the stromal matrix of intact human AM could constitute a scaffold for human MSC derived from either bone marrow (BM) or adipose tissue (AT). For this purpose, clinical-grade AM, MSC, and culture medium were used. We performed experiments of short-term adherence and proliferation for 15 days after the seeding of the cells. Morphological aspects and secretion profiles of MSC onto AM were studied, respectively, by scanning electron microscopy and Luminex analysis. Results demonstrated that the stromal matrix allow the adherence in much greater amount of MSC from BM or AT compared to 2D material. Experiments of proliferation showed that both kinds of MSC could proliferate on the stromal matrix and remain viable 15 days after the seeding of the cells. The 3D analysis of MSC culture demonstrated that both types of MSC invaded the stromal matrix and grew in multiple layers while retaining their fibroblastic morphology. By studying the secretion profile of MSC onto the stromal matrix, we found that both kinds of MSC secrete important cytokines and growth factors for wound healing of cutaneous lesions, such as vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor. In conclusion, these results suggest that the stromal matrix of AM seeded with MSC represents a bioactive scaffold that should be evaluated in patients with a nonhealing cutaneous wound.

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Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

Roux

Bodivit

Bartis

et al. 2015

Tissue Engineering Part A

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“…The breakthrough work of Japanese doctors has demonstrated that the addition of SCs to collagen sponge may be therapeutically effective and lead to wound closure [35]. Their study involved 20 patients with extensive wounds of various etiologies: burns, deep injury wounds, infections and decubitus ulcers.…”

Section: Treatment Of Chronic Wounds and Burnsmentioning

confidence: 99%

The Use of Stem Cells in Plastic and Reconstructive Surgery

Koźlik

Wójcicki

2014

Adv Clin Exp Med

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As part of their clinical practice, plastic surgeons perform procedures in the fields of skin and adipose tissue grafting, the reconstruction of compound tissue loss and congenital malformations, the treatment of acute and chronic wounds and burns, as well as cosmetic surgery. On account of the great expectations associated with their use in therapy, stem cells (SCs) are increasingly frequently employed in in vitro experiments, animal models and clinical trials. The most commonly utilized SCs for these purposes are adult stem cells (AS), which are present in small amounts in every tissue; among these are two types of cells: bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived stem cells (ADSCs). The aim of this review is to present current findings in experimental research on the use of stem cells in the field of plastic and reconstructive surgery. Many studies have described the progress of trials using ADSCs and BM-MSCs mainly in the treatment of compound tissue loss and chronic wounds. The use of their paracrine, proangiogenic and osteogenic functions is emphasized. However, because of the very high harvesting and culturing expenses and the limited availability of data on their safety for human use, SCs are not a first-choice therapy. Further developments in SC research and gradually decreasing costs mean that along with commercial-scale SC culturing, the use of SCs will become a viable alternative to traditional surgical procedures (Adv Clin Exp Med 2014, 23, 6, 1011-1017).

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“…Yoshikawa et al treated 20 patients suffering from non-healing wounds of various etiologies with autologous MSCs ex vivo expanded with animal or autologous sera. 75 They reported complete healing in 18 of the 20 patients, while the remaining two patients died for reasons unrelated to the transplantation of MSCs. Dash et al conducted a level 1 randomized control trial including 24 patients with non-healing ulcers of the lower limb.…”

Section: Clinical Studiesmentioning

confidence: 99%

Do Mesenchymal Stem Cells Have a Role to Play in Cutaneous Wound Healing?

Pountos¹,

Panteli²,

Georgouli³

et al. 2014

CTTT

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Cutaneous wounds represent a significant healthcare problem despite our increasing understanding of the molecular and cellular events governing this process. A major contributor to this problem is the lack of reliable therapies for the treatment of "hard-to-heal" wounds. Tissue engineering with the use of mesenchymal stem cells (MSCs) have emerged as a promising therapeutic tool with favorable early experimental and clinical results. This manuscript aims to provide an overview of the available approaches to up-regulate the cutaneous wound response with the use of MSCs.

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Wound Therapy by Marrow Mesenchymal Cell Transplantation

Cited by 261 publications

References 33 publications

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

The Use of Stem Cells in Plastic and Reconstructive Surgery

Do Mesenchymal Stem Cells Have a Role to Play in Cutaneous Wound Healing?

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