Wound-induced expression of the ferulate 5-hydroxylase gene in Camptotheca acuminata

Kim, Young Jin; Kim, Dong-Gwan; Lee, In-Cheol

doi:10.1016/j.bbagen.2005.08.015

Cited by 29 publications

(23 citation statements)

References 32 publications

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“…2), is predicted to encode a ferulate 5-hydroxylase (a cytochrome P450-dependent monooxygenase) that likely also functions in the biosynthesis of lignin as well as other secondary metabolites [49]. A member of this gene family was found to be woundinducible in Camptotheca acuminata [50], and other P450 monooxygenases have been associated with broad-spectrum antifungal defense in plants [51]. The upregulation of Cp35 is consistent with the response seen for the majority of PR genes, but it is still possible that Phytophthora may attenuate full upregulation of these genes for pathogenicity.…”

Section: Discussionmentioning

confidence: 97%

Carica papaya Genes Regulated by Phytophthora palmivora: A Model System for Genomic Studies of Compatible Phytophthora-plant Interactions

Porter

Zhu

Christopher

2009

Tropical Plant Biol.

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Plant pathogenic Phytophthora species are predicted to encode a large arsenal of 'effector' proteins that target and disrupt normal host cell function, but genes regulated by effectors are difficult to distinguish from those more generally regulated by microbe associated molecular patterns (MAMPs). To help make this distinction, expression studies of host-Phytophthora interactions can be compared to reveal patterns of gene regulation indicative of effector activity. The recently sequenced tropical fruit tree, Carica papaya, contains fewer genes than Arabidopsis and is highly susceptible to the broad-host-range pathogen P. palmivora, offering a new system for comparative genomics. In this study, genes isolated from the transcriptome of C. papaya seedling roots inoculated with P. palmivora, were evaluated for pathogen-regulation. Of these genes, a predicted peroxidase, ß-1,3-glucanase, ferulate 5-hydroxylase, and hypersensitive-induced response protein were pathogen upregulated, while a second peroxidase (Cp9) and aquaporin (Cp15), both with normally high root expression, were downregulated. Genes from other plants with similar expression responses to Phytophthora were identified from previously reported studies and evaluated for protein sequence similarity. Interestingly, as for papaya, Phytophthora also downregulates homologs of Cp9 and Cp15 in Glycine max and Solanum tuberosum, respectively. Because peroxidases associated with defense are generally upregulated during infection and the regulation of aquaporins have been cited in cases of both plant (Opperman et al. Science 263:221-223, 1994) and animal disease (Guttman et al. Cell Microbiol 9:131-141, 2007), Cp9 and Cp15 are potential cross-species effector targets. With whole-genome C. papaya microarrays forthcoming, this study highlights papaya as a resource for comparative genomic studies of Phytophthora-plant interaction.

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Section: Discussionmentioning

confidence: 97%

Carica papaya Genes Regulated by Phytophthora palmivora: A Model System for Genomic Studies of Compatible Phytophthora-plant Interactions

Porter

Zhu

Christopher

2009

Tropical Plant Biol.

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“…The f5h gene has been implicated in lignin biosynthesis (Chapple et al 1992), UV protection (Li et al 1993) and wound-response, and stress alleviation (Kim et al 2006) in planta. Disruption of F5H can limit lignin production leading to a dwarf Xoral architecture in the f5 h mutant, particularly under drought and NaCl stresses (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

Huang

Bhinu

et al. 2009

Planta

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Hydrocinnamic acid esters, lignin, flavonoids, glucosinolates, and salicylic acid protect plants against UV exposure, oxidative stress, diseases, and herbivores. Through the phenylpropanoid pathway, certain Brassicaceae family members, including Arabidopsis thaliana and Brassica napus, accumulate large amounts of the anti-nutritive sinapoylcholine (sinapine) in the seed. We successfully down-regulated activities of key enzymes in the pathway including F5H and SCT and achieved reduction of sinapine and lignin in B. napus seeds. Despite this success, it was unclear how multiple agronomic traits were affected in the transgenic plants. Here, we report altered large-scale gene expression of new alleles of f5h and sct mutants of A. thaliana and resultant accumulation of sinapoylglucose, disinapoylglucose, quercetin-3-O-rhamnoside, salicylic acid glucoside, and total indolyl glucosinolates in the two mutants. Expression of several flowering genes was altered in these mutants when grown under drought and NaCl treatments. Furthermore, both mutants were more susceptible to fungal infection than the wild type. Microarray experiments identified distinctive spatial and temporal expression patterns of gene clusters involved in silique/seed developmental processes and metabolite biosynthesis in these mutants. Taken together, these findings suggest that both f5h and sct mutants exhibit major differences in accumulation of diverse metabolites in the seed and profound changes in global large-scale gene expression, resulting in differential pleiotropic responses to environmental cues. Electronic supplementary material The online version of this article (doi:10.1007/s00425-009-1007-2) contains supplementary material, which is available to authorized users.

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“…The genes involved in ABA biosynthesis were induced by drought and cold treatments, and they were not responsive to exogenous ABA treatment [60]. When detached leaves were treated with ABA, F5H was found to be induced in Camptotheca acuminata [62], suggesting that F5H might belong to the ABA-dependent pathway. Similar response pattern was observed when HcF5H was treated with ABA, indicating that HcF5H can be categorized into an ABA-dependent pathway.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Ferulate 5‐Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.)

Kim

Choi

Park

et al. 2013

The Scientific World Journal

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The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.). Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF) encoding 518 amino acids (GenBank Accession number JX524278). The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78%) with that of Populus trichocarpa. Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old) of stem tissues, with a certain level of expression in all tissues tested. The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h), wounding (24 h), cold (24 h), abscisic acid (24 h), and methyl jasmonate (24 h).

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Wound-induced expression of the ferulate 5-hydroxylase gene in Camptotheca acuminata

Cited by 29 publications

References 32 publications

Carica papaya Genes Regulated by Phytophthora palmivora: A Model System for Genomic Studies of Compatible Phytophthora-plant Interactions

Carica papaya Genes Regulated by Phytophthora palmivora: A Model System for Genomic Studies of Compatible Phytophthora-plant Interactions

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

Molecular Characterization of Ferulate 5‐Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.)

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