Wound-Induced Deposition of Polyphenols in Transgenic Plants Overexpressing Peroxidase

Lagrimini, L. Mark

doi:10.1104/pp.96.2.577

Cited by 297 publications

(164 citation statements)

References 23 publications

(35 reference statements)

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“…Therefore, released acidic PODs probably catalyze the formation of brown depositions in the cell wall. Observations of Lagrimini (1991) support this hypothesis, because transgenic plants overexpressing acidic PODs showed more rapid woundinduced browning of tissues than control plants.…”

Section: Pods In the Leaf Apoplast And Their Role In Mn Toxicitysupporting

confidence: 66%

The Role of Hydrogen Peroxide-Producing and Hydrogen Peroxide-Consuming Peroxidases in the Leaf Apoplast of Cowpea in Manganese Tolerance

et al. 2006

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The apoplast is considered the leaf compartment decisive for manganese (Mn) toxicity and tolerance in cowpea (Vigna unguiculata). Particularly apoplastic peroxidases (PODs) were proposed to be key enzymes in Mn toxicity-induced processes. The presented work focuses on the characterization of the role of hydrogen peroxide (H 2 O 2 )-producing (NADH peroxidase) and H 2 O 2 -consuming peroxidase (guaiacol POD) in the apoplastic washing fluid (AWF) of leaves for early stages of Mn toxicity and genotypic differences in Mn tolerance of cowpea. Leaf AWF of the Mn-sensitive cultivar (cv) TVu 91 but not of the Mntolerant cv 1987 showed an increase of guaiacol-POD and NADH-peroxidase activities at elevated AWF Mn concentrations. two-dimensional resolutions of AWF proteins revealed that cv TVu 91 expressed more and additional proteins at high Mn treatment, whereas Mn-tolerant cv TVu 1987 remained nearly unaffected. In both cultivars, NADH-peroxidase activity and accompanied H 2 O 2 formation rate in vitro were significantly affected by Mn 21 , p-coumaric acid, and metabolites occurring in the AWF. The total phenol concentration in the AWF was indicative of advanced stages of Mn toxicity but was rather unrelated to early stages of Mn toxicity and genotypic differences in Mn tolerance. The NADH oxidation by AWF PODs was significantly delayed or enhanced in the presence of the protein-free AWF from cv TVu 1987 or cv TVu 91, respectively. High-performance liquid chromatography analysis of AWF indicates the presence of phenols in cv TVu 1987 not observed in cv TVu 91. We conclude from our studies that the H 2 O 2 -producing NADH peroxidase and its modulation by stimulating or inhibiting phenolic compounds in the leaf apoplast play a major role for Mn toxicity and Mn tolerance in cowpea.

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Section: Pods In the Leaf Apoplast And Their Role In Mn Toxicitysupporting

confidence: 66%

The Role of Hydrogen Peroxide-Producing and Hydrogen Peroxide-Consuming Peroxidases in the Leaf Apoplast of Cowpea in Manganese Tolerance

et al. 2006

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“…The determination of SOD and POD followed the same principles as that by Beauchamp and Fridovich and Lagrimini. 22,23 PBN Spin Trapping and EPR Analysis. Maize roots after 3 days of exposure to 0.05 and 1.0 mg L À1 (rac)-, (S)-, and (R)-DCPP solutions were subjected to detection of ROS generated.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Enantioselective Oxidative Damage of Chiral Pesticide Dichlorprop to Maize

Huang

et al. 2011

J. Agric. Food Chem.

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To investigate the enantioselective oxidative damage of the pesticide dichlorprop (DCPP) to maize, young seedlings were exposed to solutions of DCPP enantiomers and racemate at different concentrations. Early root development was more influenced by (R)-DCPP than racemic (rac)-and (S)-DCPP. Inhibition rates of seed germination, seedling biomass, and root and shoot elongation were all in the order of (R)-DCPP > (rac)-DCPP > (S)-DCPP treatments. The antioxidant enzyme activities of superoxide dismutase (SOD) and peroxidase (POD) were significantly upregulated by exposure to lower concentrations of (R)-DCPP than (rac)-and (S)-DCPP. Direct determination of the formation of hydroxyl radical (•OH) with electron paramagnetic resonance (EPR) spectroscopy indicated that the •OH level in maize roots followed the order of (R)-DCPP > (rac)-DCPP > (S)-DCPP treatments. All of these results provide solicited evidence of the significant enantioselective phytotoxicity of DCPP to maize with a higher toxicity of (R)-DCPP than (S)-and (rac)-DCPP.

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“…Perhaps in response to this uncertainty about the actual enzyme(s) involved, more recent attempts to establish a precise physiological function for particular peroxidase or laccase isoenzymes have taken a different approach. Transgenic plants were obtained in which genes encoding supposed lignin-forming peroxidase (95)(96)(97) and laccase (98) isoenzymes had been introduced in either sense and/or antisense orientations. Although each study was carefully executed, the results obtained, for example, in tobacco (Nicotiana tabacum) and yellow poplar (Liriodendron tulipifera), by targeting peroxidase and laccase, respectively, were equivocal.…”

Section: Oxidases and Peroxidases Involved In Lignificationmentioning

confidence: 99%

Lignin and Lignan Biosynthesis: Distinctions and Reconciliations

Lewis

Davin

Sarkanen

1998

ACS Symposium Series

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Before 1996, the framework within which lignin biosynthesis was understood at the molecular level had not fundamentally changed for 4 decades. During the same period nothing at all had been explicitly proposed about the mechanistic basis for lignan formation in vivo. The associated deficit in plant biochemistry was not minor: lignins and lignans together account for roughly 30% of the organic carbon in vascular plants. On the other hand, the biochemical transformations in phenylpropanoid metabolism leading, via the shikimate-chorismate pathway through phenylalanine or tyrosine, to the so-called monolignols (namely, the monomeric lignin/lignan precursors) came to be reasonably well documented. Indeed, attention has more recently been drawn to the identification of de facto rate-limiting steps in the various metabolic segments of the pathway as potential control-points for biotechnological manipulation. The most curious characteristic usually attributed to the lignin/lignan biosynthetic pathway is that the monolignol-derived radical coupling processes leading to lignans are regio-and stereospecific, whereas those resulting in lignin macromolecules ostensibly are not. Now that the molecular basis for the dehydrogenative dimerization of monolignols to lignans has been unraveled, however, it appears likely that an analogous mechanism may be operative in the dehydrogenative polymerization of monolignols to lignins. The investigation of this possibility has indeed become a central concern in the field of lignin biosynthesis.It has been almost three decades (7) since a comprehensive attempt had been made to summarize and evaluate contending views about lignin biosynthesis. Accordingly, the present volume draws together the current, yet conceptually vastly differing, ideas about how lignin composition and structure are established in living plants. It differs from all prior contributions, not only in introducing fresh evidence to support a compelling new paradigm that seeks to understand how lignin structure is established in vivo, but also in including lignan biosynthesis for comparative purposes. Indeed it appears that the first committed step in lignan biosynthesis bears an important relationship to the manner in which the configurations of lignins are specified in vivo.

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Wound-Induced Deposition of Polyphenols in Transgenic Plants Overexpressing Peroxidase

Cited by 297 publications

References 23 publications

The Role of Hydrogen Peroxide-Producing and Hydrogen Peroxide-Consuming Peroxidases in the Leaf Apoplast of Cowpea in Manganese Tolerance

The Role of Hydrogen Peroxide-Producing and Hydrogen Peroxide-Consuming Peroxidases in the Leaf Apoplast of Cowpea in Manganese Tolerance

Enantioselective Oxidative Damage of Chiral Pesticide Dichlorprop to Maize

Lignin and Lignan Biosynthesis: Distinctions and Reconciliations

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