Wound healing revised: A novel reepithelialization mechanism revealed by in vitro and in silico models

Safferling, Kai; Sütterlin, Thomas; Westphal, Kathi; Ernst, Claudia; Breuhahn, Kai; James, Merlin; Jäger, Dirk; Halama, Niels; Grabe, Niels

doi:10.1084/jem.21013oia63

Cited by 35 publications

(56 citation statements)

References 26 publications

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“…A recent study using cultured human skin equivalents and time-lapse fluorescent labeling found that none of the previously proposed models of epithelialization were true. In fact, they demonstrate that basal cells migrate below the suprabasal cells, which remain in place to act as a shield (Safferling et al, 2013). In this study, proliferating cells in the unwounded tissue, and not the advancing epithelial leading edge, constitute the supply of new cells covering the wound.…”

Section: Epithelializationmentioning

confidence: 66%

Palatogenesis and cutaneous repair: A two‐headed coin

Biggs

Goudy

Dunnwald

2014

Developmental Dynamics

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The reparative mechanism that operates following post-natal cutaneous injury is a fundamental survival function that requires a well-orchestrated series of molecular and cellular events. At the end, the body will have closed the hole using processes like cellular proliferation, migration, differentiation and fusion. These processes are similar to those occurring during embryogenesis and tissue morphogenesis. Palatogenesis, the formation of the palate from two independent palatal shelves growing towards each other and fusing, intuitively, shares many similarities with the closure of a cutaneous wound from the two migrating epithelial fronts. In this review, we summarize the current information on cutaneous development, wound healing, palatogenesis and orofacial clefting and propose that orofacial clefting and wound healing are conserved processes that share common pathways and gene regulatory networks.

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Section: Epithelializationmentioning

confidence: 66%

Palatogenesis and cutaneous repair: A two‐headed coin

Biggs

Goudy

Dunnwald

2014

Developmental Dynamics

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“…The 3D models of fibrous tissues can be engineered to recreate various stromal tissues, [27][28][29][30] and these can be adapted for wound models. Wound-like inclusions have been created during fabrication, [31][32][33][34] but this approach does not capture in vivo-like features of wounding, including retraction of the wound edge in tissues under tension. 35 To address these issues, Sakar and colleagues recently developed a 3D bioengineered wounding model, in which fibroblast/collagen tissues contract around anchoring pillars to form prestressed microtissues [36][37][38][39] that were then microsurgically injured using a robotically controlled micromanipulator.…”

Section: Introductionmentioning

confidence: 99%

Robust and Precise Wounding and Analysis of Engineered Contractile Tissues

Dubois

Kalashnikov

Moraes

2019

Tissue Engineering Part C: Methods

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“…Rapid migration and proliferation of keratinocytes are required for the wound healing process [23]. Re-epithelialization of the epithelium occurs by proliferation and migration of epithelial cells atop of the wound bed providing cover for the new tissue [24]. Hence, the effect of thyme oleoresin on cell migratory activity of HaCaT keratinocytes was investigated and analyzed using scratch assay.…”

Section: Anitha Et Almentioning

confidence: 99%

The Wound Healing Property of Thyme Oleoresin From Thymus Vulgaris L. On Hacat Keratinocytes

Anitha

Subeeksha

Thangavelu

2018

Asian J Pharm Clin Res

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Objective:The objective of the present study was to evaluate the wound healing property of thyme oleoresin using HaCaT keratinocytes. Methods:The effect of thyme oleoresin on cell migratory activity of HaCaT keratinocytes was investigated and analyzed using scratch assay. The HaCaT cell line was obtained from NCCS Pune and maintained in Dulbecco's Modified Eagle's Media. The keratinocyte cells (HaCaT) were trypsinized for 30 s and passaged to T25 flasks in complete aseptic environment. The effect of thyme oleoresin on wound closure was determined using a 12-well plate. Dulbecco's Modified Eagle's medium with dimethyl sulfoxide was used as control. The effect of thyme oleoresin on wound closure was determined microscopically at 20× magnification using Nikon microscope. The experiment was performed in triplicate. The wound area was photographed and analyzed.Results: Thyme oleoresin at 25 µg/ml and 50 µg/ml has significantly promoted the migration of HaCaT cells, thereby leading to wound closure. Conclusion:The study has proved the wound healing property of thyme oleoresin, and hence, it may be used for wound healing purpose in a natural way.

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Wound healing revised: A novel reepithelialization mechanism revealed by in vitro and in silico models

Cited by 35 publications

References 26 publications

Palatogenesis and cutaneous repair: A two‐headed coin

Palatogenesis and cutaneous repair: A two‐headed coin

Robust and Precise Wounding and Analysis of Engineered Contractile Tissues

The Wound Healing Property of Thyme Oleoresin From Thymus Vulgaris L. On Hacat Keratinocytes

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