Wound Healing Properties of Pelargonium Graveolens L'Hér Extract Lipogel: In-Vivo Evaluation in an Animal Burn Model

Abstract

Background: Pelargonium graveolens L'Hér has traditionally been used to reduce skin inflammation, and recent studies have confirmed antioxidant compounds in the plant's extract. The present study aimed to prepare a lipogel formulation from P. graveolens hydroalcoholic extract and evaluate its efficacy on the wound healing process in an animal model. Material and Methods: The aerial part extract of P. graveolens was prepared through percolation. Additionally, plastibase was prepared by mixing 5% of low-molecular-weight polyethylene with hot mineral oil (130 ˚C). The extract (5%) was levigated in the mineral oil (5-15%) and dispersed in the cooled plastibase. The physical properties of the lipogel, thermal stability, and microbial limits were tested. Further, the effect of the lipogel on the wound healing rate was examined among male Wistar rats, and skin tissue samples were assessed histologically. Results and Discussion: The results represented the best rheological and thermal stability characteristics in the formulation with 5% mineral oil (as the levigator). The lipogel-treated group had the least burn area compared to the silver sulfadiazine and negative control groups (p<0.05). The microscopic examination of tissue samples revealed increased collagen fiber production and maturation and significantly also faster epithelial repair among lipogel-treated rats than the other two groups (p<0.05). Conclusion: The results indicated the significant therapeutic effects of P. graveolens lipogel on burn healing. The suitable physicochemical properties and low lipogel production cost facilitate further scale-up studies.

Essential Oil Plants Traditionally Used in Wound Treatment: Systematic Review

“…: The potential treatment of wounds and ulcers using Pelargonium essential oil was observed, and it is also used traditionally for this purpose. 63 In a conducted study, a cream formulation containing P. graveolens essential oil was applied to a wound model. The results of the experiment showed that there was no significant difference in the antibacterial properties between the positive control group and the group treated with the essential oil.…”