Background
In view of the gravity of the problem of antimicrobial resistance among pathogenic bacteria against conventional bactericidal agents, investigation on alternative approaches to combat bacterial infections is warranted.
Objective
Current study aimed at investigating anti-infective potential of a polyherbal ayurvedic formulation namely panchvalkal against three different pathogenic bacteria.
Materials and methods
The panchvalkal formulation available as Pentaphyte P5® was tested for its possible
in vitro
quorum-modulatory potential against
Chromobacterium violaceum
,
Serratia marcescens
, and
Staphylococcus aureus
through broth dilution assay.
In
vivo
efficacy was demonstrated employing
Caenorhabditis elegans
as the model host for test pathogens.
Results
This formulation was found to exert quorum-modulatory effect on
C. violaceum
,
S. marcescens
, and
S. aureus
at 250–750 μg/ml. Besides altering production of the quorum sensing-regulated pigments in these bacteria, the test formulation also had
in vitro
effect on antibiotic susceptibility, catalase activity and haemolytic potential of the pathogens.
In
vivo
assay confirmed the protective effect of this panchvalkal formulation on
C. elegans,
when challenged with the pathogenic bacteria. Repeated exposure of
S. aureus
to panchvalkal did not induce resistance in this bacterium.
Conclusion
To the best of our awareness, this the first report on quorum-modulatory potential of panchvalkal formulation, validating the anti-infective potential and moderate prebiotic property of this polyherbal preparation.