Wound Healing Potential of Low Temperature Plasma in Human Primary Epidermal Keratinocytes

Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.

Section: Introductionmentioning

confidence: 99%

Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing

Shin

Park

Choi

et al. 2020

“…3 , TEWL values of the AD + plasma group was significantly lower than that of the AD group at different time points. We believe that the effect of CAP treatment on TEWL is closely linked to its ability to promote wound healing 7 , 8 , 17 , 18 . Because TEWL reflects the epidermal integrity, epithelial regeneration by proliferation of both epidermal keratinocytes as well as dermal fibroblasts would restore the function of the epidermis as a physical barrier and consequently reduce water loss.…”

Section: Discussionmentioning

confidence: 97%

Treatment of atopic dermatitis using non-thermal atmospheric plasma in an animal model

Moon

Yun

Yoon

et al. 2021

Cold atmospheric plasma (CAP) has been incorporated into various fields, including promotion of cutaneous wound healing. Atopic dermatitis (AD) is a chronic cutaneous condition characterized by inflammation-induced skin wounds and impaired skin barrier function. To investigate whether CAP may improve AD using an animal model. Dermatophagoides farinae extracts (DFE)-induced murine models of AD were used in this study. The plasma-treated group received a total of 6 CAP treatments during 2 weeks, while the control group did not receive any treatment. Differences in dermatitis severity, transepidermal water loss (TEWL), serum level of immunoglobulin (Ig) E and epidermal thickness were evaluated in both groups. The dermatitis severity was significantly improved by CAP treatment. TEWL was lower in the plasma-treated group compared with the non-treated control group. Serum Ig E dropped significantly after treatment with CAP. Difference in epidermal thickness of the ear skin was not significant between the plasma-treated and non-treated groups. Localized treatment of AD with CAP decreases dermatitis severity, TEWL, and serum Ig E level. These results show CAP’s potentials as a novel therapeutic modality for AD.

“…Written informed consent was obtained from all subjects. After that, the procedure of primary cell culture was as published previously 21 . The hypertrophic scar tissue and normal skin tissue are paired from four patients that were obtained during surgical procedure, washed with 70% ethanol and cold phosphate-buffered saline containing antibiotics and antimycotics (Gibco, Life Technologies, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted using a ReliaPrepTM RNA Miniprep system (Promega, Madison, WI, USA) according to the manufacturer’s instructions. RNA concentration was measured, cDNA was synthesized, and quantitative real-time PCR were performed as previously described 12 , 21 . The primer sequences are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…The cell lysates were centrifuged for 15 min at 13,000× g and 4 °C, and the protein concentration was measured with a BCA kit (Thermo Fisher Scientific). Electrophoresis, primary and secondary antibody attachment, development (protein detection), and quantification were performed as previously described 12 , 21 . The primary antibodies are listed in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars

Cui

Joo

Cho

et al. 2021

Self Cite

Hypertrophic scars represent a common complication in burn patients. In addition to cosmetic defects, they may cause serious sensory abnormalities such as pain and itching, severe dysfunction depending on the site, and emotional disorders such as anxiety and depression. The present study aimed to identify the molecular mechanisms underlying the use of extracorporeal shock wave therapy in keratinocytes. Keratinocytes derived from hypertrophic scar tissue were cultured and expression of proliferation markers (keratin 5 and 14), activation markers (keratin 6 and 17), differentiation markers (keratin 1, 10, and involucrin), apoptosis factors (Bax, Bcl2, and Caspase 14), and proliferation/differentiation regulators (p21 and p27) was investigated to compared with that of those in keratinocytes derived from normal skin tissue. Scar-derived keratinocytes were treated with extracorporeal shock waves under 1000 impulses at 0.1, 0.2, and 0.3 mJ/mm2. Shock waves altered the molecular pattern of proliferation, activation, differentiation, and apoptosis, as well as proliferation/ differentiation regulators, including Bax, Bcl2, ASK1, p21, p27, and Notch1. In summary, we show that extracorporeal shock wave therapy regulates the proliferation and differentiation of keratinocytes derived from hypertrophic scar to maintain normal epidermal integrity.