Wound Healing Potential of an Oleoresin Essential Oil Chemotype from Canarium schweinfurthii Engl.

Abstract

This study was conducted to investigate the chemical composition of essential oil (EO) extracted from an oleoresin of Canarium schweinfurthii widespread in the Gabonese tropical forest. A great variability in the chemical composition of EO was observed, among which a chemical profile rich in terpinolene and α-phellandrene (31.2 and 21.8%, respectively), was found and tested as a natural active ingredient for topical applications. After the evaluation of eye and skin irritancy and sensitization potentials of EO on in vitro and in chemico models, the in vitro modulating potential on a model of wound re-epithelialization was assessed. The terpinolene and α-phellandrene-rich chemotype have been proven to accelerate wound healing in a dose-dependent manner (concentration range from 1.8 to 9.0 μg/mL). In addition, the ability of this EO to modulate the pro-inflammatory response in human keratinocytes stimulated by UVB was observed in vitro by the reduction in levels of interleukin 6 (IL-6) and tumour necrosis factor-alpha (TNF-α), suggesting a possible implication during the inflammation phase of wound healing. Despite the high variability in EO composition, a method of solid-phase microextraction (SPME) of the oleoresin headspace is proposed for the in situ identification of the terpinolene and α-phellandrene-rich chemotype instead of conducting hydrodistillation. These results offer interesting perspectives for the development of innovative natural ingredients for the topical route, ingredients obtained in an eco-responsible and non-destructive way.

Biological Activity of Bursera schlechtendalii Essential oil and the Roles of Its Chemical Components in the Wound Healing Process

“…Essential oils are a complex mixture of volatile, heat-labile, and aromatic compounds. They are generated in small quantities since they constitute 0.5 to 6% of the total metabolites present in a plant [ 20 ]. Terpenes are the main chemical components in essential oils, although trace amounts of phenolic compounds, alcohols, coumarins, acids, and esters can also be found [ 2 ].…”

Natural Compounds and Biomimetic Engineering to Influence Fibroblast Behavior in Wound Healing

“…Flavonoids such as quercetin and polysaccharides such as APS2-1 from Astragalus membranaceus upregulate the expression of TGF-β1, bFGF, and epidermal growth factor (EGF) [ 31 , 67 ]. Additionally, terpenoids like terpinolene and alpha phellandrene, along with alkaloids like taspine, are associated with enhanced fibroblast migration.…”