Wound healing of rabbit cornea in organ culture

Abstract

A 2 mm perforating cut was made in the central part of 28 rabbit corneas. The corneas were then kept in organ culture from 3 h to 21 days. The healing process was studied by light microscopy and by transmission and scanning electron microscopy. The swelling of the corneal stroma adjacent to the wound created contact between the opposing wound edges within 3 h. Epithelial and endothelial cell migration into the wound was observed after 23 h. Penetration of cells through the perforation was avoided because the opposing wound edges were touching each other, thereby creating contact inhibition of cell proliferation. Cell cover of both the anterior and the posterior surface was completed within 3 days.

Proliferation of Human Corneal Endothelia in Organ Culture Stimulated by Wounding and the Engineered Human Fibroblast Growth Factor 1 Derivative TTHX1114

“…The presence of clear cell boundaries with high ZO-1 staining right up to and over the cut edge suggests that the endothelial layer may be migrating over the cut edge toward the anterior cornea while retaining the differentiated phenotype of a barrier layer as has been observed in rabbit corneas. 42,43 Figure 3 shows an example of this in a TTHX1114-treated cornea, with the endothelial cell layer extending down the cut edge toward the anterior cornea. The retention of the ZO-1-positive cell/cell contacts can be seen along with an increase in the area of the CECs.…”

A method for registration of toxic drug effects on corneal endothelium

“…ding, the specimens (except the contaminated ones) were prepared for light microscopy and for SEM (scanning electron microscopy) as described in a previous paper (Medin & Davanger 1987).…”

Endothelial repair of posterior corneal wounds in rabbits. Influence of foetal calf serum in the culture medium

“…The cell-covering process has been found to be similar if the defect also includes Descemet's membrane (Medin & Davanger 1987). The cut edge of the membrane is curled against the stroma, and endothelial cells proliferate and migrate from the edge onto the stromal surface.…”