Wound healing induces VEGF expression stimulated by forest honey in palatoplasty Sprague Dawley

Abstract

Background: Cleft palate is a craniofacial disorder with definitive therapy using the V–Y pushback technique palatoplasty, which has the impact of leaving the bone exposed on the palate with long wound healing and a high risk of infection. Forest honey has high antioxidants and the ability to accelerate wound healing. Purpose: This study aims to determine the effect of forest honey on vascular endothelial growth factor (VEGF) expression to accelerate the wound healing process after palatoplasty biopsy. Methods: Posttest only control group design using Sprague Dawley palatoplasty was performed on 15 rats which were divided into three groups, namely the honey treatment (KP), Aloclair as a positive control (KPP), and aquadest as a negative control (KKN). As much as 25 mg of honey was given therapeutically, and VEGF expression analysis post-biopsy palatoplasty was measured using the ELISA test. ANOVA analysis was carried out to determine the significant differences between each treatment, and in silico analysis was conducted to determine the compounds’ role in honey on the mechanism of VEGF expression. Results: Statistical analysis of VEGF expression in the KP group was 41.10 ng/ml ± 0.26, the KKP was 39.57 ± 0.27, while the KKN was 33.26 ± 0.62 (p≤ 0.01). In silico study, genistein (C15H10O5) targets several signaling pathways such as PI3K-Akt, AMPK, and mTOR, affecting accelerated proliferation and angiogenesis. Conclusion: In wound healing acceleration, forest honey induced VEGF expression through the genistein mechanism of angiogenesis and cell proliferation.

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“…Malnutrition and vitamin C deficiency can interfere with collagen synthesis and protein deficiency. 20 Aging can decrease liver function so that the synthesis of clotting factors is disrupted, the inflammatory response is slow, circulation to the wound area is impaired, there is a decrease in the formation of lymphocytes and antibodies, and less elastic scar tissue is formed. Low oxygen pressure in the arteries can cause disruption of collagen synthesis and epithelial formation so the necessary level of oxygen in tissues is not met.…”

“…Hypoxia can inhibit mitosis in migrating epithelial cells and fibroblasts, collagen synthesis, and the ability of macrophages to play a role in destroying ingested bacteria. 20 Sudden unexpected stress on the wound can inhibit the formation of endothelial cells and collagen tissue. 21 Wound healing is generally categorized into four stages: hemostasis, inflammation, proliferation, and maturation (remodeling).…”