Wound Healing in Colonic Anastomosis in Hypothyroidism

Abstract

Colonic anastomosis healing in hypothyroidism was evaluated. Twenty Wistar rats were separated into two groups: the study group (n = 10), which underwent total thyroidectomy to induce hypothyroidism, and the control group (n = 10). After 10 weeks, hypothyroid status was confirmed by blood tests (p < 0.0001). Three days later, a colonic anastomosis was performed, and the animals were sacrificed 1 week later. The colon containing the anastomosis was resected for bursting strength tests and histological studies. The slides were stained with hematoxylin-eosin and with Sirius red to quantify and classify the collagen. Reduced values were found for the bursting strength test (p = 0.0006) and for collagen density in the experimental group. There was a prevalence of immature type III collagen (p < 0.0001) and a decrease in mature type I collagen (p < 0.0001) in the hypothyroid group.

Wound Complications in Thyroxine-Supplemented Patients Following Foot and Ankle Surgery

“…In animal models, decreased collagen bursting strength and decreased collagen density has been observed in hypothyroid rats in colonic anastomosis 40 , and decreased collagen deposition in tracheal sutures. 44 In addition to its role in wound healing, thyroid hormone is one of the critical systemic factors for fracture repair and hypothyroidism has been shown to inhibit endochondral ossification, resulting in an impaired fracture-repair process.…”

“…31 Previous studies have shown that the composition of collagen may be altered in hypothyroidism, with a prevalence of immature, type III collagen seen compared to type I collagen in experimental animal models of hypothyroidism. 40 The quality of the collagen itself may also be altered in hypothyroidism, and shorter and thinner collagen fibers have been observed in pig models. 7…”

The Effects of Topical Application of Thyroid Hormone (Liothyronine, T3) on Skin Wounds in Diabetic Wistar Rats

“…Although the main action of thyroid hormones in skin is not well identified, several lines of evidence indicate an important role for thyroid hormones in promotion of wound healing. First, hypothyroidism is associated with retarded wound repair probably through decreasing collagen synthesis and or keratinocyte proliferation [21,23,24] which is reversible with thyroid hormone replacement [21,25]. Second, in vitro and in vivo studies have designated that thyroid hormones stimulate keratinocyte and epidermal proliferation as well as hair growth [20,21,26].…”

Combined Effect of Low-Level Laser Treatment and Levothyroxine on Wound Healing in Rats With Hypothyroidism

“…5 While wound healing depends on connective tissue activity and cellular proliferation, synthesis of connective tissue in the skin, such as collagen, and tissue tensile strength decrease in those with hypothyroidism. 6 T3 in culture medium can induce fibroblast and keratinocyte proliferation. 5 Wound healing is a complicated process that involves clot formation, inflammation, granulation, epithelialization, and remodeling.…”