Wound healing in aged skin exhibits systems-level alterations in cellular composition and cell-cell communication

Vu, Remy; Jin, Suoqin; Sun, Peng; Haensel, Daniel; Nguyen, Quy; Dragan, Morgan; Kessenbrock, Kai; Nie, Qing; Dai, Xing

doi:10.1016/j.celrep.2022.111155

Cited by 70 publications

(61 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As monocytes in ME/CFS patients show significant dysregulation in pro-inflammatory chemokine and cytokine signaling pathways, they may be intrinsically biased towards this pre-migratory transcriptome, or alternatively, they may be responding differentially to changes in intercellular signaling in ME/CFS individuals. To assess whether intercellular signaling is altered in ME/CFS, we used CellChat (Vu et al, 2022) to analyze scRNA-seq data to model communication between cell types as a function of ligand and receptor expression levels corresponding to established signaling pathways (Jin et al, 2021) (Figure 5A). To remove potentially confounding factors such as sex, exercise and cluster size, we focused our analysis on the larger female-only cohort at baseline and down-sampled large clusters to 500 cells before calculating communication probabilities, an approach recommended for similar analyses (Andrijevic et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…Inference of cell-cell communication CellChat (v1.1.3) (Vu et al, 2022) was used to infer cell-cell communication probabilities and identify signaling changes across healthy and diseased cell populations. We down-sampled each cluster to 500 cells from females, at baseline only, for a balanced comparison.…”

Section: Differential Expression Between Cells Predicted Diseased And...mentioning

confidence: 99%

“…Interaction strength along specific intercellular signaling pathways was calculated by summarizing the communication probabilities of associated ligand-receptor pairs across all clusters. Comparison of healthy and diseased signaling networks was performed as described previously (Vu et al, 2022), with statistical significance of changes in communication probabilities determined by Wilcoxon rank-sum test and using only cells collected from females at baseline.…”

Section: Differential Expression Between Cells Predicted Diseased And...mentioning

confidence: 99%

See 2 more Smart Citations

Single-cell transcriptomics of the immune system in ME/CFS at baseline and following symptom provocation

Ahmed

Zhu

et al. 2022

Preprint

View full text Add to dashboard Cite

ME/CFS is a serious and poorly understood disease. To understand immune dysregulation in ME/CFS, we used single-cell RNA-seq (scRNA-seq) to examine immune cells in cohorts of patients and controls. Post-exertional malaise (PEM), an exacerbation of symptoms following strenuous exercise, is a characteristic symptom of ME/CFS. Thus, to detect changes coincident with PEM, we also performed scRNA-seq on the same cohorts following exercise. At baseline, ME/CFS patients displayed dysregulation of classical monocytes suggestive of inappropriate differentiation and migration to tissue. We were able to identify both diseased and more normal monocytes within patients, and the fraction of diseased cells correlated with metrics of disease severity. Comparing the transcriptome at baseline and post-exercise challenge, we discovered patterns indicative of improper platelet activation in patients, with minimal changes elsewhere in the immune system. Taken together, these data identify immunological defects present at baseline in patients and an additional layer of dysregulation following exercise.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Differential Expression Between Cells Predicted Diseased And...mentioning

confidence: 99%

Section: Differential Expression Between Cells Predicted Diseased And...mentioning

confidence: 99%

See 1 more Smart Citation

Single-cell transcriptomics of the immune system in ME/CFS at baseline and following symptom provocation

Ahmed

Zhu

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Also, the wound induced plasticity of myeloid lineage cells was demonstrated on this model (Guerrero-Juarez et al, 2019). Major variations in epithelial, fibroblast, and immune cell populations were observed in young and aged skin during wound healing (Vu et al, 2022). It is well known that wound healing declines with age, which contributes to a variety of health complications, and to decreased lifespan.…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that wound healing declines with age, which contributes to a variety of health complications, and to decreased lifespan. Aged skin wounds exhibited more inflammatory profile than young equivalents, probably due to dysregulated growth factor, chemokine, and cytokine pathways during wound healing in aged skin (Vu et al, 2022). Moreover, aged basal epidermal keratinocytes isolated from the wound edge appeared to be more recalcitrant to activation, as judged by their markedly reduced transcriptional activity of genes involved in important processes of wound-repair (Keyes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Signaling pathways in cutaneous wound healing

et al. 2022

View full text Add to dashboard Cite

Wound healing is a very complex process, where variety of different pathways is activated, depending on the phase of healing. Improper or interrupted healing might result in development of chronic wounds. Therefore, novel approaches based on detailed knowledge of signalling pathways that are activated during acute or chronic cutaneous wound healing enables quicker and more effective healing. This review outlined new possibilities of cutaneous wound healing by modulation of some signalling molecules, e.g., gasotransmitters, or calcium. Special focus is given to gasotransmitters, since these bioactive signalling molecules that can freely diffuse into the cell and exert antioxidative effects. Calcium is an important booster of immune system and it can significantly contribute to healing process. Special interest is given to chronic wounds caused by diabetes mellitus and overcoming problems with the inflammation.

show abstract

Immunomodulatory Hydrogels: Advanced Regenerative Tools for Diabetic Foot Ulcer

Xiong

Qian

Lü

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Diabetic foot ulcer (DFU) is one of the most common complications of diabetes, bringing physical and mental challenges for patients due to the lack of efficient curative therapy. Despite considerable advances in pharmacological and surgical approaches, clinical trials for DFU patients remain disappointing due to the local overactive and excessive inflammation. Immunomodulatory hydrogels has significant advantages to overcome the clinical challenge of DFUs therapy. Here, recent fabrication and regenerative advances in the utilization of functional hydrogels for altering the immune microenvironment of DFUs are comprehensively reviewed. The pathological features and the healing processes of DFUs, followed by summarizing the physicochemical properties essential for the design of regenerative hydrogels for immunomodulation in DFUs, are briefly introduced. Then, the potential immuno-therapeutic modalities of hydrogels and emerging trends used to treat DFUs via multitherapeutic approaches and enhanced efficacy and safety are discussed. Taken together, by linking the structural properties of hydrogels to their functions in DFU therapy with a particular focus on immunomodulatory stimuli, this review can promote further advances in designing advanced hydrogels for DFUs, resulting in improved diabetic wound repair through translation into clinical setting in the near future.

show abstract

Wound healing in aged skin exhibits systems-level alterations in cellular composition and cell-cell communication

Cited by 70 publications

References 98 publications

Single-cell transcriptomics of the immune system in ME/CFS at baseline and following symptom provocation

Single-cell transcriptomics of the immune system in ME/CFS at baseline and following symptom provocation

Signaling pathways in cutaneous wound healing

Immunomodulatory Hydrogels: Advanced Regenerative Tools for Diabetic Foot Ulcer

Contact Info

Product

Resources

About