Wound healing effects of glucose oxidase – peroxidase incorporated alginate diamine PEG-g-poly (PEGMA) xerogels under high glucose conditions: An in vitro evaluation

In Vivo Wound-Healing Efficacy of Insulin-Loaded Strontium-Cross-Linked Alginate-Based Hydrogels in Diabetic Rats

“…It was further cross-linked with an optimized concentration of strontium ions (100 mM) and lyophilized. The material ADPM2S2 was further evaluated by various physicochemical characterization techniques, and the results were discussed in our previous published work . The hydrogel was cross-linked with varying concentrations of strontium ions (50, 100, 150 and 200 mM).…”

“…The material ADPM2S2 was further evaluated by various physicochemical characterization techniques, and the results were discussed in our previous published work. 13 concentration showed a stable swelling at different pH values as well as a lower dissolution rate. Hence, it was taken as the optimized crosslinking concentration for hydrogel synthesis.…”

“…Materials used for the synthesis of alginate diamine PEG-g-poly(PEGMA) hydrogels have been already reported in our previous work. 13 For in vitro studies, the minimum essential medium (MEM), 0.25% trypsin−EDTA, 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT), collagen from bovine achilles tendon, collagenase from Clostridium histolyticum (824 units/mg collagen), and lipopolysaccharides (LPSs) from Escherichia coli O111:B4 were purchased from Sigma-Aldrich. Fetal bovine serum (FBS) and Dulbecco's modified Eagle medium with high glucose were obtained from GIBCO.…”

“…In our previous work, we developed alginate- g -poly­(methacrylic acid) hydrogels for the controlled delivery of insulin and established its controlled delivery as well as in vitro wound-healing effects . However, the stability of the insulin activity was not maintained for a prolonged time period of 48 h. Hence, in another approach, the mechanical stability of alginate hydrogels was improved by conjugation with diamine PEG, followed by grafting with poly­(PEGMA) chains, and the biological activity of the loaded enzymes was retained for a longer period than that by the previous hydrogels . We hypothesized that the alginate diamine PEG- g -poly­(PEGMA) hydrogels could release insulin without any change in its conformation and could exhibit enhanced wound healing in in vivo diabetic models.…”

Preparation, Characterization, and Evaluation of Enzyme Co-Modified Fish Gelatin-Based Antibacterial Derivatives

“…Fang et al [25] prepared thermosensitive hydrogels loaded with glucose oxidase (GOD), which completely inhibited the growth of Staphylococcus aureus and Escherichia coli and promoted wound healing, in addition to being highly biocompatible. Division et al [26] prepared a GOD-peroxidase-conjugated alginate diamine PEG-g-poly (PEGMA) dry gel that released 57% GOD-loaded and 63% peroxidase-loaded dry gel after 24 h, resulting in a significant improvement in wound closures, demonstrating its potential for treating diabetic wounds. However, the use of GOD for treating wound infections has not been widely explored owing to its lack of stability in the wound microenvironment.…”