Wound healing biomolecules present in four proposed soft aqueous extractions of &lt;i&gt;Ageratum conyzoides&lt;/i&gt; Linn

Akpalo, Amivi Edefia; Saloufou, Issa Kouassi; Eloh, Kodjo; Kpegba, Kafui

doi:10.4314/ijbcs.v14i2.26

Cited by 5 publications

(6 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TLC profiling for phenols detection showed that after the TLC plates were dipped in FeCl3 reagent and heated at 110 o C indicated a positive results because a grey to black colour was formed (Figure 2d) [19]. The TLC profiling for terpenoids detection gave the results after the TLC plates were sprayed with Vanillin-H2SO4 reagent and heated at 110 o C. The formation of blue to purple or brown color indicates a positive result (Figure 2e) [22]. In the terpenoid and phenol detection, the result showed that several crude extracts contained spots of the same Rf value.…”

Section: Resultsmentioning

confidence: 98%

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Octaverina,

Sibero,

Subagiyo

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The exploration of fungal secondary metabolites has increased significantly since the first discovery of penicillin. In addition, pigment derived from marine fungi attracts plenty of attention due to its complex and unusual chemical structures, which have tremendous antioxidants and antimicrobial activities. Hence, our current work aimed to characterize the crude pigment extract of an unidentified fungus SUCCF0001 and its potential as an antioxidant and antimicrobial agent. The fungus was cultivated on two semi-solid media namely MEA and PDA for 14 days at 25 °C, and two different liquids media namely MEB and PDB for 14 days at 25 °C with agitation at 150 r.p.m. The semi-solid medium and the fungal mycelium from the broth culture were extracted with methanol, while the liquid medium using ethyl acetate. The crude extracts were characterized using TLC plates and then visualized using DPPH, vanillin-H2SO4, and FeCL3. The presence of carotenoid pigment was detected using TLC with β-carotene as a marker. Antioxidant bioassay was screened using the DPPH method; while antimicrobial activity bioassay was conducted using the paper disc method against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Candida tropicalis. The results showed that only the PDB extract had radical scavenging activity (RSA) > 50% for antioxidant activity. On the other hand, antimicrobial bioassay showed that only MEB extract had antimicrobial activity against B. subtilis. TLC analysis indicated the presence of antioxidants, terpenoids and phenols in both PDB and MEB extract. However, the result of fungal pigment characterization using TLC concluded that the pigment was not a β-carotene.

show abstract

Section: Resultsmentioning

confidence: 98%

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Octaverina,

Sibero,

Subagiyo

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Research works conducted in our laboratory on Ageratum conyzoides Linn. plant extract, demonstrated this extract contains flavonoids which are serine proteases inhibitors (Akpalo et al, 2020a).…”

Section: Discussionmentioning

confidence: 93%

“…Fibrin soft gels are formed subsequent to the thrombin hydrolysis of the fibrinogen molecules and fibrin polymerization. In our mind, phenomenon occurring, is certainly syneresis due first, to thrombin inhibition by plant extract substances previously demonstrated (Akpalo et al, 2020a); secondly, osmotic pressure leading to exudate of incorporated extract mixing with gel liquid phase and its molecules (ions, not attached fibrinogen and fibrin molecules,…). The stirring has pronounced this phenomenon.…”

Section: Discussionmentioning

confidence: 97%

“…The fibrin scaffold is then less organized and probably contains less entrapped or attached protein molecules (small molecular weights products are present in electrophoresis). The extract certainly prevented normal hydrolysis of fibrinogen to fibrin as it contains thrombin inhibitors (Akpalo et al, 2020a). As consequences, normal fibrin molecules interactions to form the scaffold were disturbed.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fibrin – Fibrinogen scaffold structural modification by phytochemicals and phytoproteases contained in an aqueous extract of Ageratum conyzoides Linn.

Akpalo¹,

Awaga²,

Tete-Benissan³

2021

Int. J. Bio. Chem. Sci

Self Cite

View full text Add to dashboard Cite

Based on mechanisms of fibrin clot polymerization and dissolution, it is possible to modulate fibrin formation and removal. Ageratum conyzoides Linn. (Asteraceae) is an annual herb with a long history of traditional medicine. There is high variability in the secondary metabolites of this plant which include flavonoids, and these molecules belong to a class of serine proteases inhibitors. Several plant enzymes belonging to the classes of serine proteases were observed to be active on the cascade of coagulation pathways. The aim of this study was to observe if even Ageratum conyzoides Linn. aqueous leaves extract contained proteases which could structurally modify the fibrin clot formation. To prepare plant extracts, dry leaves of the plant were extracted with distilled water. Fibrin gels were prepared by mixtures containing fibrinogen and thrombin with or without extract. Fibrin networks were disrupted by a denaturation buffer. Samples were deposited in 8% polyacrylamide gel and Coomassie blue was used to reveal migration. Our extract contained phytochemicals class flavonoids which are thrombin inhibitors. But our results support the evidence that the same extract contained plant serine proteases, specifically a fibrinogenase which hydrolyzed fibrinogen but not like thrombin.Keywords: Fibrin/Fibrinogen, structural modification, Ageratum conyzoides Linn., phytoproteases.

show abstract

“…TLC analysis using vanillin-sulfuric acid reagent performed the positive result of terpenoids for all extracts of G. verrucosa (F, A, 3D, 6D and 9D) which indicated by the presence of purple spot on TLC plate. Akpalo et al (2020) express that purple spot detected on TLC plate was expected as terpenoids.…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

et al. 2022

View full text Add to dashboard Cite

Abstract. Fatmawati F, Sibero T, Trianto A, Wiyanti DP, Sabdono A, Pronggenies D, Radjasa OK. 2022. The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa. Biodiversitas 23: 5258-5266. Gracilaria verrrucosa is producer of agar, a polysaccharide, that widely used in pharmaceutical and food industries. However, several studies have shown that the secondary metabolite of this seaweed is not potential as an antimicrobial and antioxidant agents. This study aimed to determine the antibacterial and antioxidant properties of methanolic extract of G. verrucosa after fermentation using marine yeast Hortaea werneckii SUCCY001. The samples were fermented based on different optimization times, specially in three, six and nine days. Samples were extracted using methanol by maceration method. Then the extracts were tested for antimicrobial potential against the several human clinical pathogens namely Bacillus cereus, Bacillus subtilis, Escherichia coli, Micrococcus luteus and Pseudomonas aeruginosa. The antioxidant potential was analyzed using the 1,1-diphenyl-2-picrylhydracyl (DPPH) and 2,2-Azinobis 3-ethyl benzothiazoline 6- sulfonic acid (ABTS) method while total phenolic content (TPC) was measured using Folin-Ciocalteu. Secondary metabolites were characterized using thin layer chromatography (TLC) and GC-MS. TLC plate visualization using UV light, DPPH, FeCl3 and vanillin-sulfuric acid reagents. The results showed that the extract fermented for nine days was able to inhibit the growth of P. aeruginosa and M. luteus at a concentration of 2 mg/mL. The highest antioxidant activity was found in the nine days fermented extract with an RSA value of 21.9% ± 0,0001 for DPPH and 12.53 % ± 0.002 for ABTS. Furthermore, the highest total phenolic content was found in the nine days fermented extract of 7.57 GAE/mg. The results of characterization using TLC showed the presence of phenolic compounds, terpenoids and antioxidants in fresh seaweed extracts, autoclaved fresh seaweed, three days of fermentation, six days and nine days. Based on this research, it can be seen that the addition of marine yeast starter H. werneckii SUCCY001 was able to increase the antibacterial and antioxidant activity of G. verrucosa.

show abstract

Wound healing biomolecules present in four proposed soft aqueous extractions of <i>Ageratum conyzoides</i> Linn

Cited by 5 publications

References 4 publications

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Antioxidant and antimicrobial screening of an unidentified yellow pigmented marine-derived fungus

Fibrin – Fibrinogen scaffold structural modification by phytochemicals and phytoproteases contained in an aqueous extract of Ageratum conyzoides Linn.

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

Contact Info

Product

Resources

About